##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512315_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1823658 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.002659489882422 31.0 30.0 34.0 16.0 34.0 2 29.918415623982128 31.0 30.0 34.0 22.0 34.0 3 30.599276838091352 33.0 31.0 34.0 26.0 34.0 4 34.26293087848708 37.0 35.0 37.0 31.0 37.0 5 34.44478844169247 37.0 35.0 37.0 32.0 37.0 6 34.59732910447025 37.0 35.0 37.0 32.0 37.0 7 34.538653080785984 37.0 35.0 37.0 32.0 37.0 8 34.54143649741344 37.0 35.0 37.0 32.0 37.0 9 36.13082003314218 39.0 37.0 39.0 32.0 39.0 10 36.066188945515 39.0 37.0 39.0 32.0 39.0 11 36.08689238881413 39.0 37.0 39.0 32.0 39.0 12 36.02910688297916 39.0 37.0 39.0 32.0 39.0 13 36.000540671551356 39.0 37.0 39.0 32.0 39.0 14 37.24504375272118 40.0 38.0 41.0 32.0 41.0 15 37.17449763058644 40.0 38.0 41.0 31.0 41.0 16 37.103803454375765 40.0 38.0 41.0 31.0 41.0 17 37.06624542540323 40.0 38.0 41.0 31.0 41.0 18 36.854740856015766 40.0 38.0 41.0 31.0 41.0 19 36.961136353417146 40.0 38.0 41.0 31.0 41.0 20 36.895935531771855 40.0 38.0 41.0 31.0 41.0 21 36.84795668924765 40.0 38.0 41.0 31.0 41.0 22 36.76800200476186 40.0 38.0 41.0 31.0 41.0 23 36.65203234378376 40.0 38.0 41.0 30.0 41.0 24 36.527371908548645 40.0 37.0 41.0 30.0 41.0 25 36.416163556982724 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2280769509720209 1101 2 0.3565055094936369 1101 3 -0.11295295930933236 1101 4 -0.14126605521486368 1101 5 -0.34435599616902124 1101 6 -0.331846494925216 1101 7 -0.5247856713466561 1101 8 -0.5988309575929378 1101 9 -0.572293016915836 1101 10 -0.6115746755512177 1101 11 -0.7645419224217278 1101 12 -0.8156657365431954 1101 13 -0.882067419228548 1101 14 -1.100024521183613 1101 15 -1.1641917378372 1101 16 -1.184981343458432 1101 17 -1.315919459666702 1101 18 -1.0975286045480814 1101 19 -1.1793476291216791 1101 20 -1.2904168846379065 1101 21 -1.151830929749039 1101 22 -1.3655240958803745 1101 23 -1.1668759724118303 1101 24 -1.5717480527495482 1101 25 -1.4576758188374086 1102 1 0.9745172782550107 1102 2 0.08159503484649377 1102 3 -0.2599166934027579 1102 4 -0.380347579352339 1102 5 -0.4444496812857466 1102 6 -0.5932044588835765 1102 7 -0.5790072890969142 1102 8 -0.7852484242605726 1102 9 -0.7713416970046794 1102 10 -0.7896751465088272 1102 11 -0.8368592364441128 1102 12 -0.909973009257314 1102 13 -0.7063481417976192 1102 14 -1.1443799024711865 1102 15 -1.264889131292989 1102 16 -1.2275133012965114 1102 17 -1.3274250048128025 1102 18 -1.2788624780478557 1102 19 -1.3036019462331367 1102 20 -1.408792189385828 1102 21 -1.5015625345497696 1102 22 -1.3926396070972729 1102 23 -1.6285352479618282 1102 24 -1.4776766205609349 1102 25 -1.3321677850673623 1103 1 1.3153462085594576 1103 2 0.5225821756803981 1103 3 -0.050350097383560666 1103 4 -0.026962636881933122 1103 5 -0.28332270489791966 1103 6 -0.20690542439898252 1103 7 -0.27198599203591556 1103 8 -0.20307049214184048 1103 9 -0.3657575618391533 1103 10 -0.3888346738009787 1103 11 -0.3893446674765926 1103 12 -0.5140851563101876 1103 13 -0.6189907219891282 1103 14 -0.7689396825021646 1103 15 -0.8239364849980007 1103 16 -0.8012629571803203 1103 17 -0.8839572422811699 1103 18 -0.7437019291866704 1103 19 -1.0621471390542965 1103 20 -0.8366796973875665 1103 21 -1.0211070076291051 1103 22 -0.862257215232674 1103 23 -1.0161026201081498 1103 24 -0.9943126069886645 1103 25 -1.047214037600554 1104 1 1.4699219239997916 1104 2 0.7878189193016709 1104 3 0.2583148608832211 1104 4 0.19113989009431265 1104 5 0.07273119779989656 1104 6 0.002724052010925959 1104 7 -0.09949889122116673 1104 8 -0.16904230737418402 1104 9 -0.1816315079747639 1104 10 -0.18329616393937442 1104 11 -0.24358866222115694 1104 12 -0.06969625401457336 1104 13 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24 -0.7106880862907161 1105 25 -0.9103591119997745 1106 1 0.9841766901925411 1106 2 0.28133287017996267 1106 3 -0.2723423945802388 1106 4 -0.3651036080096546 1106 5 -0.4610767972484098 1106 6 -0.5794860146952701 1106 7 -0.557842012354989 1106 8 -0.5672585040399909 1106 9 -0.9315243082551561 1106 10 -0.9223123874517185 1106 11 -0.8431240679581151 1106 12 -0.918402959310427 1106 13 -0.8031795653969596 1106 14 -1.0458172880566465 1106 15 -1.0931396341519388 1106 16 -1.1751210487144164 1106 17 -1.2217297034364378 1106 18 -0.9504492522924366 1106 19 -0.9209567951494506 1106 20 -1.1637767081102623 1106 21 -1.240258943345843 1106 22 -1.2501621509985483 1106 23 -1.4609962057668753 1106 24 -1.3150712461852692 1106 25 -1.2491971881907418 1107 1 0.3163397818512479 1107 2 -0.19728793937598965 1107 3 -0.572170437719933 1107 4 -0.333619577936922 1107 5 -0.31850600566246356 1107 6 -0.3352985072077672 1107 7 -0.37356985592537484 1107 8 -0.30565543365309367 1107 9 -0.29543148369206307 1107 10 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-0.5158596627970411 1108 22 -0.7839421683902401 1108 23 -0.7061465993096547 1108 24 -0.5751606573204171 1108 25 -0.7473613984667011 1109 1 1.0455596387960817 1109 2 0.3886056430657412 1109 3 -0.15328223194364554 1109 4 -0.21216500350184475 1109 5 -0.08647684813954015 1109 6 -0.1760322270689798 1109 7 -0.09157172309982542 1109 8 -0.20762664045689405 1109 9 -0.3865501659678543 1109 10 -0.3170555485078168 1109 11 -0.2669343085441511 1109 12 -0.2171034434929595 1109 13 -0.3877556100091297 1109 14 -0.6028565709934242 1109 15 -0.7782847993151663 1109 16 -0.6250900481302395 1109 17 -0.5954103866678082 1109 18 -0.572057555431563 1109 19 -0.6851355305406486 1109 20 -0.7975612522583191 1109 21 -0.7743846731139357 1109 22 -0.7921993015252013 1109 23 -0.8121951936784413 1109 24 -0.8721875825971637 1109 25 -0.9126741993760348 1110 1 0.7118049026643796 1110 2 0.16513781156309193 1110 3 -0.23797037793075404 1110 4 -0.29126544403516164 1110 5 -0.19701829493810408 1110 6 -0.15008593068412068 1110 7 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14 0.9895474137642921 1201 15 1.089508980215811 1201 16 1.159544679263739 1201 17 1.1552012505930378 1201 18 1.3407546157248547 1201 19 1.1987676881354972 1201 20 1.138329716669567 1201 21 1.25205505959822 1201 22 1.016586359997099 1201 23 1.3173462720217017 1201 24 1.41101983375259 1201 25 1.1082414334111235 1202 1 2.509627601792623 1202 2 1.55292708452302 1202 3 1.322041163715351 1202 4 1.0261624415854556 1202 5 0.902618449871639 1202 6 0.8127379811533189 1202 7 0.7607047295936553 1202 8 0.700220019726288 1202 9 0.8827807717742502 1202 10 0.8878920433552295 1202 11 0.8634999564690489 1202 12 0.9235706919971349 1202 13 0.9563157595621021 1202 14 1.0571736852505893 1202 15 1.1707740886396607 1202 16 1.1166925061780688 1202 17 1.1019987850766668 1202 18 1.298666598098734 1202 19 1.2207111406314866 1202 20 1.1250417371025492 1202 21 1.1607915072367234 1202 22 1.1958685296039278 1202 23 1.2312999885824638 1202 24 1.1127236724692011 1202 25 1.2858595083142177 1203 1 2.6124930244178444 1203 2 1.7107359407919738 1203 3 1.1490588145324914 1203 4 0.8567229019808238 1203 5 0.6526836467536441 1203 6 0.7977950760054213 1203 7 0.7403878397161492 1203 8 0.7531822231512564 1203 9 0.8150747928819939 1203 10 0.8618614214257363 1203 11 0.8047035365249968 1203 12 0.8590142966155625 1203 13 0.836708658465561 1203 14 1.0632161542950485 1203 15 1.014417966754678 1203 16 0.9629961654140189 1203 17 0.8771172011746557 1203 18 1.0624541264149414 1203 19 0.9723926539415615 1203 20 1.0107560857330071 1203 21 0.946591201652879 1203 22 1.0836408661815327 1203 23 1.1016527249482948 1203 24 1.087551227109536 1203 25 0.9926339755479461 1204 1 2.336695099132868 1204 2 1.5017894189611525 1204 3 1.0073252147465652 1204 4 0.7753239195493293 1204 5 0.5585237078573684 1204 6 0.5369266280130063 1204 7 0.5061341304586122 1204 8 0.511841390866941 1204 9 0.6980479170465017 1204 10 0.6463546958213016 1204 11 0.7139898385737808 1204 12 0.6984486499787224 1204 13 0.5207101021770626 1204 14 0.663876929941452 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-0.3961917918882776 2314 11 -1.5584628531271676 2314 12 -0.9685130780152491 2314 13 -0.693315901178984 2314 14 -0.6241753351486636 2314 15 -0.7539675413281302 2314 16 -0.8415030072966161 2314 17 -1.180603845011646 2314 18 -1.9232715969408005 2314 19 -1.8688759081366726 2314 20 -2.283450335914523 2314 21 -1.6900251685903314 2314 22 -1.2112045116723351 2314 23 -1.126349774814571 2314 24 -1.0334027072263865 2314 25 -0.8024562979768675 2315 1 -2.6158908163036543 2315 2 -1.231579922040087 2315 3 -0.5648782291336474 2315 4 -0.5076667148395515 2315 5 -0.41129162623339965 2315 6 -0.3289094692010224 2315 7 -0.30823326295632825 2315 8 -0.38461471998770236 2315 9 -0.47015742349726963 2315 10 -0.4461719321830344 2315 11 -0.6530767525157444 2315 12 -0.5890064900045715 2315 13 -0.5966705820743741 2315 14 -0.5675803037179818 2315 15 -1.1929674751880626 2315 16 -1.0380389300195532 2315 17 -0.8393893506453978 2315 18 -0.6600077719863293 2315 19 -0.7870429581445819 2315 20 -0.7025224791675484 2315 21 -0.6761316866410851 2315 22 -0.6189537934159475 2315 23 -0.5568897925627425 2315 24 -0.45176711637097355 2315 25 -0.30307189578582694 2316 1 1.3288585121030465 2316 2 0.5277529821523999 2316 3 -0.08336583496414463 2316 4 -0.17274198420422948 2316 5 -0.31391302217765826 2316 6 -0.525404455284253 2316 7 -0.4007755466644909 2316 8 -0.3527895015380409 2316 9 -0.5825153463585977 2316 10 -0.5813040202805624 2316 11 -0.6522236229394522 2316 12 -0.7003079262929148 2316 13 -0.6713953379247499 2316 14 -0.8870768754402647 2316 15 -0.7995413916680363 2316 16 -0.7341859968297513 2316 17 -0.6883251055938544 2316 18 -0.608603911658335 2316 19 -0.7159098323505475 2316 20 -0.6541213924956395 2316 21 -0.6220226630456125 2316 22 -0.7511619049052811 2316 23 -0.8536034101473362 2316 24 -0.7951094931808811 2316 25 -0.806166498309274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49264.0 3 1.0 4 404.0 5 1963.0 6 2348.0 7 2257.0 8 2068.0 9 1960.0 10 1928.0 11 2002.0 12 1899.0 13 1947.0 14 2019.0 15 2069.0 16 2110.0 17 2240.0 18 2600.0 19 3005.0 20 3718.0 21 4630.0 22 5608.0 23 6667.0 24 7826.0 25 9192.0 26 10421.0 27 11878.0 28 13282.0 29 16352.0 30 20756.0 31 26632.0 32 35490.0 33 48593.0 34 68210.0 35 98970.0 36 171353.0 37 292176.0 38 889820.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.18997256942247 19.447811744249073 11.87867815981269 24.483537526515768 2 14.641442479988473 20.408183469163493 41.89880992320985 23.051564127638184 3 18.719084187827313 25.296835805131362 29.79200589710866 26.192074109932665 4 12.03313065795973 16.991178285755776 39.033857825077256 31.94183323120724 5 12.78750787824658 38.15392196438648 36.10838267434711 12.950187483019834 6 30.982885779122544 37.623964307075255 18.531152147908383 12.861997765893818 7 27.33468521886513 31.745170494410402 22.928712342788277 17.99143194393619 8 24.64509023614441 37.206579872438226 20.97826696871909 17.17006292269827 9 26.596031281751287 14.94801036273988 20.48488473871029 37.97107361679855 10 14.887460726135012 28.807228809702323 35.0780750144563 21.227235449706367 11 33.8169421978283 22.30741405318873 25.457990843481586 18.417652905501388 12 23.465323130373594 24.636409613650855 32.26466125733887 19.633605998636675 13 29.14633428876036 22.238503397694988 25.92673031170601 22.688432001838642 14 20.766649330200305 22.46047340648369 28.13494074996894 28.63793651334706 15 22.720776987522527 31.35810485479633 23.714957835220314 22.20616032246083 16 21.758046453548104 28.08041333152743 28.43322508927657 21.728315125647896 17 21.363588555556717 28.67636247895756 28.262644874125332 21.69740409136039 18 21.11371507906053 27.221822385962945 30.590438295566646 21.074024239409876 19 23.27487518551279 26.706429407956833 28.098454787588885 21.920240618941495 20 23.49050995594791 27.092199516734627 28.6957768672141 20.721513660103355 21 22.807176587906607 26.602933379485023 27.76579115810525 22.82409887450311 22 23.189934672682462 28.15054937330586 27.723417668689017 20.93609828532266 23 21.96000864190676 27.84013352075789 28.478491162660806 21.72136667467454 24 22.95435872799422 26.672620904879118 29.020802963691807 21.35221740343486 25 22.376312298028594 27.408269886097763 28.719109041162255 21.49630877471139 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 977.0 1 977.0 2 1379.5 3 1782.0 4 1782.0 5 1782.0 6 2897.5 7 4013.0 8 4013.0 9 4013.0 10 4763.5 11 5514.0 12 5514.0 13 5514.0 14 7655.5 15 9797.0 16 9797.0 17 9797.0 18 16729.0 19 23661.0 20 23661.0 21 23661.0 22 36843.5 23 50026.0 24 50026.0 25 50026.0 26 71689.5 27 93353.0 28 93353.0 29 93353.0 30 119631.0 31 145909.0 32 145909.0 33 145909.0 34 162329.5 35 178750.0 36 178750.0 37 178750.0 38 191241.5 39 203733.0 40 203733.0 41 203733.0 42 213142.5 43 222552.0 44 222552.0 45 222552.0 46 227713.5 47 232875.0 48 232875.0 49 232875.0 50 224844.0 51 216813.0 52 216813.0 53 216813.0 54 191831.0 55 166849.0 56 166849.0 57 166849.0 58 143091.5 59 119334.0 60 119334.0 61 119334.0 62 97844.5 63 76355.0 64 76355.0 65 76355.0 66 58673.0 67 40991.0 68 40991.0 69 40991.0 70 30001.0 71 19011.0 72 19011.0 73 19011.0 74 13133.0 75 7255.0 76 7255.0 77 7255.0 78 4980.5 79 2706.0 80 2706.0 81 2706.0 82 1820.0 83 934.0 84 934.0 85 934.0 86 605.5 87 277.0 88 277.0 89 277.0 90 190.5 91 104.0 92 104.0 93 104.0 94 74.5 95 45.0 96 45.0 97 45.0 98 43.5 99 42.0 100 42.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3478174087465961 2 0.299178903061868 3 0.31902911620490243 4 0.30948785353394115 5 0.29391475813995827 6 0.25147258970706127 7 0.35330089304025203 8 0.32199019772347665 9 0.265071630755328 10 0.2402862817480032 11 0.2536659834245237 12 0.25103391096356886 13 0.2700616014625549 14 0.2607945130062764 15 0.3500108024640585 16 0.4053391589870469 17 0.4214057679674589 18 0.11400163846510694 19 0.12913605511559734 20 0.1941153439954202 21 0.19592489381232664 22 0.14662837001235976 23 0.2530628001522215 24 0.3545072595848564 25 0.39415285102798886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1823658.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.97927175818748 #Duplication Level Percentage of deduplicated Percentage of total 1 81.24666953695171 55.23089427900093 2 12.344504564536486 16.78340861025623 3 3.4309446101201355 6.996993481159358 4 1.2598044261993084 3.4256234980308093 5 0.5586140353046023 1.8987087656954644 6 0.3102380328678526 1.2653853327629554 7 0.19641796190579594 0.9346645007408392 8 0.12373648611004667 0.6729212972542442 9 0.08395275713559149 0.5136342562952538 >10 0.394493936769861 4.819859017086403 >50 0.027103741714559336 1.2868870778294241 >100 0.01954651217869418 2.6418014450731477 >500 0.0025137825380491118 1.1223844029913033 >1k 0.0013785259079624145 2.0438409955163555 >5k 8.108975929190674E-5 0.3629930403073388 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6585 0.36108744073724347 No Hit GGTATCAACGCAGAGTACTTTTTTT 4009 0.2198328853326665 No Hit TATCAACGCAGAGTACTTTTTTTTT 3642 0.19970849797494925 No Hit GTCCTACAGTGGACATTTCTAAATT 3459 0.1896737217175589 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3436 0.188412520330018 No Hit CTGTAGGACGTGGAATATGGCAAGA 3107 0.17037185700389 No Hit CTTTAGGACGTGAAATATGGCGAGG 2780 0.15244086336363508 No Hit GTCCTACAGTGTGCATTTCTCATTT 2430 0.1332486683358393 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1845 0.10117028521795204 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGGCGT 105 5.456968E-12 15.3829155 6 CGTGCGC 65 3.4139757E-6 14.598335 10 GGTATCA 1235 0.0 14.30873 1 TGCGGAC 40 0.0053205937 14.233377 10 GCGCCAC 105 1.9945219E-8 12.666537 13 GCGTGCG 75 1.5069583E-5 12.643537 9 AGGCGTG 125 1.4078978E-9 12.161887 7 GGCGTGC 95 1.0654167E-6 11.9761095 8 TAGGACC 780 0.0 11.694446 4 GTTTAGG 175 1.8189894E-12 11.400827 1 GGCGAGG 785 0.0 11.264909 19 TGTAGGA 2175 0.0 11.227341 2 AGAACCG 70 0.0014893379 10.859127 5 GGTATAC 80 3.7688029E-4 10.688275 1 GTAGGAC 2185 0.0 10.610635 3 GCACCGT 100 2.3956014E-5 10.451335 6 CTGTAGG 2100 0.0 10.450759 1 GTGCGCC 100 2.425653E-5 10.439535 11 GTCCTAC 1840 0.0 10.430103 1 AGGACCT 1340 0.0 10.210821 5 >>END_MODULE