FastQCFastQC Report
Thu 26 May 2016
SRR1512315_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512315_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1823658
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74280.40731321333276305No Hit
GGTATCAACGCAGAGTACTTTTTTT48460.26572964887056677No Hit
TATCAACGCAGAGTACTTTTTTTTT45720.25070490190594946No Hit
GTCCTACAGTGGACATTTCTAAATT35770.19614423318407287No Hit
GTCCTAAAGTGTGTATTTCTCATTT33700.1847934206962051No Hit
CTGTAGGACGTGGAATATGGCAAGA33190.18199684370644056No Hit
CTTTAGGACGTGAAATATGGCGAGG30200.16560122566840932No Hit
GTCCTACAGTGTGCATTTCTCATTT24700.13544206205330164No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22080.12107533320392311No Hit
CTGTAGGACCTGGAATATGGCGAGA19080.10462488032295528No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19040.10440554095120906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.014.6276141
TCCAACG1257.2759576E-1213.67034618
CCGTTTC853.953741E-612.29150813
GTATTAG1900.012.0145921
TAGGACC7500.011.5305464
GCCCTAA1001.901979E-611.4138631
ACCGTGC855.2922423E-511.1829958
AGGACCT14300.010.6335335
GCGCCAC909.536723E-510.55331613
TGTAGGA20950.010.523252
GTCCTAC20000.010.3675911
TAGGACT2750.010.3671254
GGACCTG13900.010.3261686
CCTACAC2800.010.1817183
TTAGGAC12800.010.1711123
AGGACGT18900.010.1574145
GTCCTAA10750.010.0866691
GTAGGAC20250.010.0426093
CCGTGCA951.6372403E-410.004199
CGCCCTA951.6559301E-49.99294316
Produced by FastQC (version 0.11.5)