FastQCFastQC Report
Thu 26 May 2016
SRR1512314_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512314_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792915
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51060.28478762239146865No Hit
GGTATCAACGCAGAGTACTTTTTTT34260.1910854669630183No Hit
TATCAACGCAGAGTACTTTTTTTTT30640.17089488347188797No Hit
GTCCTACAGTGGACATTTCTAAATT30450.1698351567140662No Hit
GTCCTAAAGTGTGTATTTCTCATTT26660.14869639665014794No Hit
CTGTAGGACGTGGAATATGGCAAGA25360.14144563462294643No Hit
CTTTAGGACGTGAAATATGGCGAGG23310.1300117406569748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCG250.006085989518.9709389
TAGGACC5850.012.6689294
GGTATCA7500.012.4162471
AGGACCT10450.012.27491955
CGTTGAT701.088687E-412.2171522
TACACCG752.070705E-411.4020365
GGACCTG10700.011.2773996
GTATCAA18050.010.9499371
TGTAGGA17700.010.8980382
TCCAACG909.512258E-510.55596118
GTAGGAC17300.010.4909013
GGCGAGG6950.010.39500919
CTACACT2600.010.2325964
GTCCTAG2700.010.2060981
TAGGACT2800.010.1803894
GACCTGG11800.010.145887
CGACTAA750.00262802710.14253917
CTGTAGG17550.010.070741
AGGACGT17100.010.0573525
TGGCGAG15900.010.03812118
Produced by FastQC (version 0.11.5)