##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512314_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1792915 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.003567932668307 31.0 30.0 34.0 16.0 34.0 2 29.92033420435436 31.0 30.0 34.0 23.0 34.0 3 30.596409757294683 33.0 31.0 34.0 26.0 34.0 4 34.260397732184735 37.0 35.0 37.0 31.0 37.0 5 34.43617405175371 37.0 35.0 37.0 32.0 37.0 6 34.58158027569628 37.0 35.0 37.0 32.0 37.0 7 34.52730385991528 37.0 35.0 37.0 32.0 37.0 8 34.52800774158284 37.0 35.0 37.0 32.0 37.0 9 36.117655326660774 39.0 37.0 39.0 32.0 39.0 10 36.056989316281026 39.0 37.0 39.0 32.0 39.0 11 36.07716930250458 39.0 37.0 39.0 32.0 39.0 12 36.022308363754 39.0 37.0 39.0 32.0 39.0 13 35.9989452930005 39.0 37.0 39.0 32.0 39.0 14 37.24887794457629 40.0 38.0 41.0 32.0 41.0 15 37.18750247501973 40.0 38.0 41.0 31.0 41.0 16 37.11250338136498 40.0 38.0 41.0 31.0 41.0 17 37.073551730003935 40.0 38.0 41.0 31.0 41.0 18 36.853284734636055 40.0 38.0 41.0 31.0 41.0 19 36.962732756432956 40.0 38.0 41.0 31.0 41.0 20 36.900705276044874 40.0 38.0 41.0 31.0 41.0 21 36.84981496613057 40.0 38.0 41.0 31.0 41.0 22 36.78091487884255 40.0 38.0 41.0 31.0 41.0 23 36.67094368667784 40.0 38.0 41.0 30.0 41.0 24 36.54037252184292 40.0 37.0 41.0 30.0 41.0 25 36.431755548924514 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2070883501585854 1101 2 0.33324823475196297 1101 3 -0.18191634801305767 1101 4 -0.13909078189428215 1101 5 -0.3257525720748191 1101 6 -0.4152837726028267 1101 7 -0.4194165946016639 1101 8 -0.4311738416378077 1101 9 -0.7625542910675733 1101 10 -0.6046698503269141 1101 11 -0.6650660025778237 1101 12 -0.9012132203728527 1101 13 -0.8641661019719606 1101 14 -0.985297148613995 1101 15 -1.1856664342784882 1101 16 -1.1695586595021723 1101 17 -1.396606489510674 1101 18 -1.192362331846084 1101 19 -1.1521283796791835 1101 20 -1.2054500052104018 1101 21 -1.1236542503463696 1101 22 -1.2590578720037868 1101 23 -1.3616409630769724 1101 24 -1.5200457842053439 1101 25 -1.5696641634260473 1102 1 1.411624755601462 1102 2 0.6127902826766913 1102 3 0.07405587223044208 1102 4 0.09924015322805246 1102 5 -0.06779067401141248 1102 6 -0.2445542690587459 1102 7 -0.44291856253437345 1102 8 -0.2831199917405698 1102 9 -0.32108726078263317 1102 10 -0.5272703708599238 1102 11 -0.4502422842085281 1102 12 -0.4470549174514389 1102 13 -0.6449210023081093 1102 14 -0.7652006153098583 1102 15 -0.7586509060184667 1102 16 -0.9306990931897019 1102 17 -0.9380522995648661 1102 18 -0.7726531614821184 1102 19 -0.7735268453740218 1102 20 -0.7900336200429692 1102 21 -0.9352350975509651 1102 22 -0.9465644798936665 1102 23 -1.1775704579230108 1102 24 -1.1232795824091966 1102 25 -1.09460618270721 1103 1 0.9194306948785815 1103 2 0.15412089943043483 1103 3 -0.4106422323635073 1103 4 -0.5144870277852007 1103 5 -0.5686945566912414 1103 6 -0.6302183938636006 1103 7 -0.6881046651444294 1103 8 -0.8223462456224055 1103 9 -0.9619506483911948 1103 10 -0.7338537084203054 1103 11 -0.7807005238424054 1103 12 -0.9145785466214136 1103 13 -0.9412515063407056 1103 14 -1.1029829113268548 1103 15 -1.2713651466442926 1103 16 -1.1703999030911305 1103 17 -1.223177617297587 1103 18 -1.1378006289197984 1103 19 -1.5403166598913742 1103 20 -1.2984292318088038 1103 21 -1.5017659352381472 1103 22 -1.498004774321295 1103 23 -1.5722663644546344 1103 24 -1.4906011589271273 1103 25 -1.5199530820303195 1104 1 1.494558723740365 1104 2 0.7290610815751393 1104 3 0.1435215699737853 1104 4 0.1327554889877831 1104 5 -0.041861119442707206 1104 6 -0.06626524629867703 1104 7 -0.23927024736267555 1104 8 -0.08927309997591948 1104 9 -0.2858849846394804 1104 10 -0.3789599683788438 1104 11 -0.31232605074578856 1104 12 -0.33685396987603866 1104 13 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21 -0.9162608411817459 1108 22 -0.8295176611661716 1108 23 -0.8259631483325052 1108 24 -0.8624752606805046 1108 25 -0.8847889224697809 1109 1 0.8418342630579723 1109 2 0.1300569339235338 1109 3 -0.25482504160094166 1109 4 -0.36506039208724417 1109 5 -0.432762315083167 1109 6 -0.4391417098096184 1109 7 -0.40746473636203717 1109 8 -0.510005748819637 1109 9 -0.5503489821285825 1109 10 -0.6995786056474529 1109 11 -0.6250517971308511 1109 12 -0.594840294789492 1109 13 -0.6733535857801414 1109 14 -0.849721486401215 1109 15 -0.742224124311889 1109 16 -0.8716642632666165 1109 17 -1.001353800065118 1109 18 -0.9160022279271303 1109 19 -1.0274110753569659 1109 20 -1.1720776092099854 1109 21 -1.2460968103649037 1109 22 -1.2170914580363927 1109 23 -1.19906211955157 1109 24 -1.3116786510871918 1109 25 -1.3523548124200602 1110 1 0.16813642371281645 1110 2 -0.15094058731480686 1110 3 -0.531686726924022 1110 4 -0.4595815627695288 1110 5 -0.53088588188308 1110 6 -0.6006380923161458 1110 7 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-1.1929698461474274 2314 12 -0.7465170845897617 2314 13 -0.4851745552528328 2314 14 -0.5893552078948119 2314 15 -0.5596244810440396 2314 16 -0.7920380886216734 2314 17 -1.1682701484772835 2314 18 -2.1937257456404353 2314 19 -1.8800647513951532 2314 20 -1.9749131120059573 2314 21 -1.500797056406526 2314 22 -1.1650745986225957 2314 23 -0.953204811387387 2314 24 -0.832517848352694 2314 25 -0.7479273449671311 2315 1 -2.6214098174510596 2315 2 -1.292800108819712 2315 3 -0.6272940852282716 2315 4 -0.4785749533971213 2315 5 -0.3699901945963475 2315 6 -0.42093355863865867 2315 7 -0.40051179400171577 2315 8 -0.3427380137066365 2315 9 -0.3307231539748585 2315 10 -0.4461354897230052 2315 11 -0.5400617071233214 2315 12 -0.5304965465302516 2315 13 -0.4913848305578554 2315 14 -0.49414704825196765 2315 15 -1.1159797598901093 2315 16 -1.0204500421129623 2315 17 -0.7968705841602528 2315 18 -0.6469111170915482 2315 19 -0.7060717079792553 2315 20 -0.5794299611036635 2315 21 -0.6011038722453037 2315 22 -0.5418137446481097 2315 23 -0.6022301276161244 2315 24 -0.4783114009618572 2315 25 -0.3892497682967857 2316 1 1.2562603388872446 2316 2 0.5431111972602238 2316 3 -0.08503313533812928 2316 4 -0.21115542238668894 2316 5 -0.3664935537537559 2316 6 -0.4489028839941511 2316 7 -0.1985324760715983 2316 8 -0.3908280710568661 2316 9 -0.5228318583867448 2316 10 -0.6029410463345002 2316 11 -0.6662455218387393 2316 12 -0.6356374765518211 2316 13 -0.5384326747922898 2316 14 -0.6307773863471695 2316 15 -0.5922100148616849 2316 16 -0.6251284702655155 2316 17 -0.6061265770440585 2316 18 -0.5564764793762293 2316 19 -0.6229018342105874 2316 20 -0.6668814617638432 2316 21 -0.6059401845975287 2316 22 -0.8766945585500281 2316 23 -0.791353794280262 2316 24 -0.7895963832093926 2316 25 -0.7754011318536698 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49015.0 3 5.0 4 402.0 5 1949.0 6 2407.0 7 2107.0 8 1967.0 9 1938.0 10 1869.0 11 1916.0 12 1823.0 13 1854.0 14 1954.0 15 1962.0 16 2015.0 17 2230.0 18 2518.0 19 3035.0 20 3694.0 21 4486.0 22 5607.0 23 6714.0 24 7632.0 25 9072.0 26 10180.0 27 11384.0 28 13017.0 29 15836.0 30 20299.0 31 26198.0 32 34798.0 33 47434.0 34 66700.0 35 97083.0 36 168369.0 37 285608.0 38 877838.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07596797506435 20.16034859753756 12.349316318453523 24.414367108944568 2 14.533542581076505 20.633394037558293 41.73367909564692 23.099384285718283 3 18.097701509643453 25.240806486273527 30.335517361795095 26.325974642287925 4 12.087901019584232 17.385866831253523 39.2504956351096 31.27573651405265 5 12.876540654250052 37.970112846105216 36.39563833298832 12.757708166656409 6 30.793345975391045 37.77037497252841 18.697513883987963 12.73876516809258 7 26.99333339677681 32.02102367542616 23.350296999440765 17.635345928356266 8 24.25913012917444 37.130292587215976 21.408080873653944 17.202496409955646 9 26.093850676546392 15.292599602663232 21.13799174899771 37.47555797179267 10 14.656996916229357 29.032903804808225 35.508878072237955 20.801221206724467 11 33.26999036433765 22.625072071734515 25.68494012523565 18.41999743869219 12 22.60593306829678 25.146671394003178 32.62717812123046 19.620217416469586 13 28.499815693927744 22.60465033026865 26.146459281782448 22.749074694021157 14 20.411826130533537 22.63908332443109 28.61602101637696 28.333069528658417 15 22.403363320327152 31.499331030660628 24.0168389939149 22.08046665509732 16 21.527572012772897 28.496036895991416 28.44108947132288 21.53530161991281 17 21.140933205604387 29.010716906718653 28.51716777869279 21.331182108984162 18 20.969508187383674 27.665834795947823 30.51381465682122 20.85084235984728 19 22.812300687951193 27.31860997892249 28.45609186979974 21.412997463326583 20 22.969438610648815 27.605843805291435 28.900033642900176 20.524683941159573 21 22.40096263851482 27.1432874910088 28.05132209100019 22.404427779476194 22 22.593691172445382 28.477089024070068 28.082773336908375 20.846446466576175 23 21.610314279274974 28.177591809959385 28.616152244560354 21.59594166620529 24 22.397449850188774 27.04322132201128 29.29632391470837 21.263004913091574 25 22.130328102518664 27.715570181948117 28.81968111054047 21.334420604992747 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 916.0 1 916.0 2 1384.0 3 1852.0 4 1852.0 5 1852.0 6 2826.5 7 3801.0 8 3801.0 9 3801.0 10 4819.0 11 5837.0 12 5837.0 13 5837.0 14 8648.0 15 11459.0 16 11459.0 17 11459.0 18 18999.0 19 26539.0 20 26539.0 21 26539.0 22 40377.5 23 54216.0 24 54216.0 25 54216.0 26 75419.5 27 96623.0 28 96623.0 29 96623.0 30 121675.0 31 146727.0 32 146727.0 33 146727.0 34 165453.0 35 184179.0 36 184179.0 37 184179.0 38 195822.5 39 207466.0 40 207466.0 41 207466.0 42 215110.0 43 222754.0 44 222754.0 45 222754.0 46 224577.0 47 226400.0 48 226400.0 49 226400.0 50 215877.0 51 205354.0 52 205354.0 53 205354.0 54 181504.0 55 157654.0 56 157654.0 57 157654.0 58 134183.0 59 110712.0 60 110712.0 61 110712.0 62 89447.5 63 68183.0 64 68183.0 65 68183.0 66 52244.0 67 36305.0 68 36305.0 69 36305.0 70 26174.5 71 16044.0 72 16044.0 73 16044.0 74 11237.5 75 6431.0 76 6431.0 77 6431.0 78 4349.5 79 2268.0 80 2268.0 81 2268.0 82 1508.5 83 749.0 84 749.0 85 749.0 86 504.5 87 260.0 88 260.0 89 260.0 90 182.0 91 104.0 92 104.0 93 104.0 94 76.0 95 48.0 96 48.0 97 48.0 98 41.0 99 34.0 100 34.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3657172816335409 2 0.310109514394157 3 0.3348178803791591 4 0.3242763878934584 5 0.3081573861560643 6 0.2623660351996609 7 0.3652153058008885 8 0.3362680327845994 9 0.2794889886023598 10 0.2521034181765449 11 0.26582409093571086 12 0.2640392879751689 13 0.2860146744268412 14 0.2775368603642671 15 0.3672789842240151 16 0.42232900053822964 17 0.43978660449603024 18 0.1211435009467822 19 0.13280049528282153 20 0.19705340186233034 21 0.20447148916708266 22 0.1519871271086471 23 0.2672742433411512 24 0.3718525418104037 25 0.4130703351804185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1792915.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.74600342970648 #Duplication Level Percentage of deduplicated Percentage of total 1 83.26432950287281 61.40411529091069 2 11.682237763349052 17.230366923251715 3 2.9178703146687512 6.4554382269900135 4 0.9804891374817863 2.8922862118208705 5 0.4234021034873732 1.5612106487962383 6 0.2149195348416085 0.950967405212411 7 0.12115852221227688 0.6254469756223318 8 0.07807339387493477 0.4606080617975811 9 0.05412489036925889 0.35923449157234677 >10 0.23131510853710013 2.9676935047435355 >50 0.016994258517912923 0.8540105755674465 >100 0.012508759982531413 1.7430732777371252 >500 0.0015157116497328995 0.7526549848450526 >1k 9.85212572326382E-4 1.4575250129779522 >5k 7.578558248664477E-5 0.28536840815466175 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5106 0.28478762239146865 No Hit GGTATCAACGCAGAGTACTTTTTTT 3426 0.1910854669630183 No Hit TATCAACGCAGAGTACTTTTTTTTT 3064 0.17089488347188797 No Hit GTCCTACAGTGGACATTTCTAAATT 3045 0.1698351567140662 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2666 0.14869639665014794 No Hit CTGTAGGACGTGGAATATGGCAAGA 2536 0.14144563462294643 No Hit CTTTAGGACGTGAAATATGGCGAGG 2331 0.1300117406569748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 5.577509251693471E-5 0.0 11 0.0 0.0 0.0 5.577509251693471E-5 0.0 12 0.0 0.0 0.0 5.577509251693471E-5 0.0 13 0.0 0.0 0.0 5.577509251693471E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGGCG 25 0.0060859895 18.970938 9 TAGGACC 585 0.0 12.668929 4 GGTATCA 750 0.0 12.416247 1 AGGACCT 1045 0.0 12.2749195 5 CGTTGAT 70 1.088687E-4 12.217152 2 TACACCG 75 2.070705E-4 11.402036 5 GGACCTG 1070 0.0 11.277399 6 GTATCAA 1805 0.0 10.949937 1 TGTAGGA 1770 0.0 10.898038 2 TCCAACG 90 9.512258E-5 10.555961 18 GTAGGAC 1730 0.0 10.490901 3 GGCGAGG 695 0.0 10.395009 19 CTACACT 260 0.0 10.232596 4 GTCCTAG 270 0.0 10.206098 1 TAGGACT 280 0.0 10.180389 4 GACCTGG 1180 0.0 10.14588 7 CGACTAA 75 0.002628027 10.142539 17 CTGTAGG 1755 0.0 10.07074 1 AGGACGT 1710 0.0 10.057352 5 TGGCGAG 1590 0.0 10.038121 18 >>END_MODULE