FastQCFastQC Report
Thu 26 May 2016
SRR1512314_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512314_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792915
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58130.3242206128009415No Hit
GGTATCAACGCAGAGTACTTTTTTT39780.22187331803236626No Hit
TATCAACGCAGAGTACTTTTTTTTT38820.21651890915074054No Hit
GTCCTACAGTGGACATTTCTAAATT29690.16559624968277917No Hit
CTGTAGGACGTGGAATATGGCAAGA27780.15494320701204461No Hit
GTCCTAAAGTGTGTATTTCTCATTT26620.1484732962800802No Hit
CTTTAGGACGTGAAATATGGCGAGG25920.1445690398038948No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18060.1007298170855841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTGC456.737325E-414.7852058
GGTATCA11300.013.6377391
GGCGAGG7950.011.70252719
TAGGACC5600.011.5392574
TAGGACT2700.011.2627174
GTGGTAT2850.011.0147561
GCGCCAC951.3633538E-510.99785113
TGGCGAG16450.010.84957318
GGACCTG10850.010.5994996
TGTAGGA16050.010.5976772
TTAGGAC10750.010.5192153
AGGACCT11500.010.4951125
GTCCTAC15250.010.3548561
GTAGGAC16100.010.2699473
AGGACGT16400.010.1408745
GGACGTG15800.010.1060616
ATGGCGA18000.010.07324517
TATGGCG17900.010.02345216
ACCGTGC951.6358243E-410.0050278
CCACCTT5250.09.95043713
Produced by FastQC (version 0.11.5)