FastQCFastQC Report
Thu 26 May 2016
SRR1512313_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512313_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631337
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60690.3720261356175947No Hit
GGTATCAACGCAGAGTACTTTTTTT37290.22858550992222942No Hit
TATCAACGCAGAGTACTTTTTTTTT35150.21546743560649945No Hit
GTACTGGTTCACTATCGGTCAGTCA19310.11836916590502147No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16810.10304431273243972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10700.013.7635781
AATACCG550.003063967512.0933215
TAGGACC1900.011.0021944
TACACGG2300.010.7412725
TAGGACG1605.4205884E-1010.6896314
TGTAGGA3700.010.53012
GTAGGAC3350.010.49563
GTACCGT1303.8314647E-710.2324946
TAGACGG750.002643302810.1353555
GTATCAA23700.010.1026571
GTTCTAG1701.5552359E-910.0602091
CTGGACT3600.09.7658374
GTACTGG6150.09.7330481
TCGAACT1106.804213E-59.50394419
TCTATAC1502.6356975E-79.5027733
ATAACCC1106.8133944E-59.5027723
TAGAACC1201.695976E-59.5018954
GCGTGCG800.004573939.4852389
ACCCTAC1651.0626718E-79.213391
AGCGTAC1554.2494867E-79.1948161
Produced by FastQC (version 0.11.5)