FastQCFastQC Report
Thu 26 May 2016
SRR1512313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512313_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631337
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71530.4384746989739091No Hit
GGTATCAACGCAGAGTACTTTTTTT46440.2846744725338786No Hit
TATCAACGCAGAGTACTTTTTTTTT44680.2738857759003811No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21490.13173243787151276No Hit
GTACTGGTTCACTATCGGTCAGTCA20120.12333441833293796No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17140.1050671933512205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.013.7122351
GTAATAC500.001497870313.3020393
TTATACT2150.011.4904874
ACCGCTC752.0662659E-411.4045488
TTTATAC2100.011.3112583
TATACTG2450.010.8594745
GTCCTAA1855.456968E-1210.79671
TAATACC803.7644798E-410.6894674
GTATCAA26850.010.3792691
TTAGGAC2200.010.3652273
CCGCTCA1054.090731E-59.9554349
CGCCCTA3250.09.93236816
ATAAGAC1252.5642548E-69.88151553
GTTCTAG1701.6314516E-89.5113771
GTATAGA2201.6370905E-119.5113771
GTCTTAG1401.0410204E-69.5113771
TAGGACC1606.612572E-89.5017484
TAAGACC1401.0554922E-69.5017484
TCTATAC1401.0559343E-69.5014563
TGGTATC4450.09.3946992
Produced by FastQC (version 0.11.5)