FastQCFastQC Report
Thu 26 May 2016
SRR1512312_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512312_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703882
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89280.5239799469681586No Hit
GGTATCAACGCAGAGTACTTTTTTT55430.32531595497810295No Hit
TATCAACGCAGAGTACTTTTTTTTT51630.30301394110625035No Hit
GTCCTACAGTGGACATTTCTAAATT34970.20523721713123327No Hit
GTCCTAAAGTGTGTATTTCTCATTT33300.1954360689296559No Hit
CTGTAGGACGTGGAATATGGCAAGA32820.1926189724405798No Hit
CTTTAGGACGTGAAATATGGCGAGG29750.17460129281253045No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25470.1494821824516017No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25030.14689984400328193No Hit
GTACTGGTTCACTATCGGTCAGTCA22440.13169926086430866No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19570.11485537144004104No Hit
GTCCTACAGTGTGCATTTCTCATTT18640.10939724699245605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.014.1834721
TAGGACC5350.013.3185014
TCCTATA1701.8189894E-1211.7366422
TTAGAAC1303.2603566E-810.9627983
ATTATAC2450.010.858393
GACGTGA9750.010.8169477
TTAGGAC11050.010.7478413
GTTCTAA1951.8189894E-1210.7182311
TGTAGGA17850.010.6987082
CGTGCGC803.800039E-410.67864810
GTATTAT1701.382432E-1010.6179281
TGTTATA2250.010.5567692
ATAGGAC1803.45608E-1110.5567683
AGGACCT13900.010.5257685
GTATCAA31400.010.4986851
GGCGAGG8450.010.46145219
TGGCGAG20450.010.45475818
CTGTAGG17700.010.3590311
GTGGTAT4050.010.321261
GTAGGAC17800.010.3017453
Produced by FastQC (version 0.11.5)