##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512312_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1703882 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.7547330155492 31.0 30.0 34.0 16.0 34.0 2 29.66061499563937 31.0 30.0 34.0 19.0 34.0 3 30.336482221186678 31.0 31.0 34.0 26.0 34.0 4 33.996745079764914 37.0 35.0 37.0 30.0 37.0 5 34.16532365504184 37.0 35.0 37.0 30.0 37.0 6 34.3209699967486 37.0 35.0 37.0 31.0 37.0 7 34.261285699361814 37.0 35.0 37.0 31.0 37.0 8 34.26178221261801 37.0 35.0 37.0 31.0 37.0 9 35.81189894605377 39.0 37.0 39.0 31.0 39.0 10 35.74199152288715 39.0 37.0 39.0 30.0 39.0 11 35.7638580605934 39.0 37.0 39.0 30.0 39.0 12 35.700092494668056 39.0 37.0 39.0 30.0 39.0 13 35.6729480093105 39.0 37.0 39.0 30.0 39.0 14 36.86750667006283 40.0 38.0 41.0 31.0 41.0 15 36.78525977737895 40.0 38.0 41.0 30.0 41.0 16 36.71545740843556 40.0 38.0 41.0 30.0 41.0 17 36.68112463187005 40.0 38.0 41.0 30.0 41.0 18 36.461795476447314 40.0 37.0 41.0 29.0 41.0 19 36.57105656377613 40.0 37.0 41.0 30.0 41.0 20 36.51036221991898 40.0 37.0 41.0 30.0 41.0 21 36.45205301775592 40.0 37.0 41.0 30.0 41.0 22 36.36725606585433 40.0 37.0 41.0 29.0 41.0 23 36.23618184827353 40.0 37.0 41.0 29.0 41.0 24 36.09542738288215 40.0 37.0 41.0 28.0 41.0 25 35.98053386326048 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.250895458565374 1101 2 0.33507937066316984 1101 3 -0.14224189435066137 1101 4 -0.1907808502819961 1101 5 -0.37005358166078395 1101 6 -0.3402723430067809 1101 7 -0.39669905725575916 1101 8 -0.5711314464262855 1101 9 -0.6383027565937098 1101 10 -0.4976124283702177 1101 11 -0.5952528582909125 1101 12 -0.815337766607179 1101 13 -0.7631217958362697 1101 14 -1.0882262002625822 1101 15 -1.1908471187647578 1101 16 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0.4408997826731813 1103 3 -0.05903128849226036 1103 4 0.05027795615738739 1103 5 -0.2998873218548894 1103 6 -0.39773832672998566 1103 7 -0.33286541705452777 1103 8 -0.45198796806785424 1103 9 -0.32999519898265817 1103 10 -0.6309564696019194 1103 11 -0.5695476034291573 1103 12 -0.6968253456418978 1103 13 -0.6235801647477714 1103 14 -0.7645232830295186 1103 15 -0.9343626399028224 1103 16 -0.8798366112675922 1103 17 -0.9994782010451573 1103 18 -0.7051903048810217 1103 19 -1.134944023946474 1103 20 -1.0783118329155172 1103 21 -1.084733747883945 1103 22 -1.156944126104463 1103 23 -1.2937260565739734 1103 24 -1.4834146618750168 1103 25 -1.358174739219713 1104 1 1.2343664415719964 1104 2 0.5712397739932982 1104 3 -0.16978564925684836 1104 4 -0.11752800607489178 1104 5 -0.3478195661599912 1104 6 -0.44006006399254716 1104 7 -0.49138434256096275 1104 8 -0.6211828577500427 1104 9 -0.6508681263682732 1104 10 -0.6466555143763841 1104 11 -0.6994050136968397 1104 12 -0.7558222492323594 1104 13 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1107 11 -0.6741168309571606 1107 12 -0.5587685662518993 1107 13 -0.5472919350181229 1107 14 -0.7165164707785507 1107 15 -0.9089115465096782 1107 16 -0.8318323170985451 1107 17 -0.8899391191546115 1107 18 -0.7603493091642761 1107 19 -0.9834956651903042 1107 20 -0.9068283553100827 1107 21 -0.9048478505696451 1107 22 -1.0226166260478067 1107 23 -0.9252316261515148 1107 24 -1.0251417974812895 1107 25 -1.0771118904129082 1108 1 -0.35074809598145507 1108 2 -0.7785500460822554 1108 3 -1.1929000071321845 1108 4 -0.8193789804664178 1108 5 -0.8555334084750612 1108 6 -1.041805694843859 1108 7 -0.9458844823911079 1108 8 -1.040002401512531 1108 9 -1.2217786741981556 1108 10 -1.1162841168139224 1108 11 -1.1902128117270507 1108 12 -1.2333327799638383 1108 13 -1.160476930802382 1108 14 -1.512060434775357 1108 15 -1.6043368204913477 1108 16 -1.3785192759290936 1108 17 -1.3335418022481136 1108 18 -1.1907949627042953 1108 19 -1.3245604960736799 1108 20 -1.5868534749780778 1108 21 -1.4747347121842438 1108 22 -1.5253541538163589 1108 23 -1.6400817779265822 1108 24 -1.8090664584531595 1108 25 -1.8993877423217285 1109 1 0.2712278461537707 1109 2 -0.40417152711737714 1109 3 -0.915617481491239 1109 4 -0.8949827566690516 1109 5 -1.024319478188616 1109 6 -1.0962972348329671 1109 7 -1.1229183120809765 1109 8 -1.1441140641309033 1109 9 -1.3484127474071173 1109 10 -1.3543726989570928 1109 11 -1.3265969672479514 1109 12 -1.3953671998147854 1109 13 -1.3409098812557687 1109 14 -1.756011283675484 1109 15 -1.6104858848271917 1109 16 -1.5362051404322514 1109 17 -1.7294156444452469 1109 18 -1.799451901031425 1109 19 -1.8242001872020097 1109 20 -2.042990011333039 1109 21 -1.9785076016336163 1109 22 -2.02336417205958 1109 23 -2.0648807607787774 1109 24 -2.1286873472564594 1109 25 -2.343767648682835 1110 1 0.07899120382296942 1110 2 -0.34729393930571817 1110 3 -0.6850934409335245 1110 4 -0.7511361437453772 1110 5 -0.6260764844132751 1110 6 -0.646271723918936 1110 7 -0.5839496951435805 1110 8 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-1.1123621248837097 2314 18 -1.9303992907215246 2314 19 -1.9218731765351365 2314 20 -2.038398290574321 2314 21 -1.3485940647289425 2314 22 -1.0061633200571265 2314 23 -0.6828342710794217 2314 24 -0.643002167216217 2314 25 -0.5351659598321135 2315 1 -2.505889358175189 2315 2 -1.1194366800512547 2315 3 -0.3229928232623678 2315 4 -0.2132099740878317 2315 5 -0.16190810819417578 2315 6 -0.16401185557865716 2315 7 -0.14672200508190514 2315 8 -0.08688234546508511 2315 9 -0.15712703490494562 2315 10 -0.3040253816984091 2315 11 -0.3709040469883078 2315 12 -0.40999361167649084 2315 13 -0.2816359390296128 2315 14 -0.2802620282011432 2315 15 -0.7348713690497775 2315 16 -0.6690466690699566 2315 17 -0.5948483336594492 2315 18 -0.317709946278228 2315 19 -0.446636603606251 2315 20 -0.4882365235148356 2315 21 -0.4494670193430039 2315 22 -0.44607227670517346 2315 23 -0.495001949676535 2315 24 -0.43715241913523784 2315 25 -0.37385695334725 2316 1 1.476124912425469 2316 2 0.7411422962630709 2316 3 0.19964616249468392 2316 4 0.053269476718334374 2316 5 -0.08882206093070266 2316 6 -0.2690848796644332 2316 7 -0.3036818844225806 2316 8 -0.26307554786338727 2316 9 -0.38549470992224855 2316 10 -0.3437870740697022 2316 11 -0.5071356757492254 2316 12 -0.29871334400287 2316 13 -0.39534892045585224 2316 14 -0.40826609342081355 2316 15 -0.4200418177567613 2316 16 -0.22786006736213693 2316 17 -0.3441820343236799 2316 18 -0.22514255932209437 2316 19 -0.4331931802461213 2316 20 -0.309218867393227 2316 21 -0.3257236170463642 2316 22 -0.5762461887690193 2316 23 -0.5448430517511653 2316 24 -0.5292287229124284 2316 25 -0.4754633774048287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53953.0 3 3.0 4 486.0 5 2064.0 6 2532.0 7 2378.0 8 2189.0 9 2172.0 10 2028.0 11 2044.0 12 2037.0 13 2097.0 14 2038.0 15 2129.0 16 2149.0 17 2284.0 18 2625.0 19 3223.0 20 3955.0 21 4755.0 22 5710.0 23 6737.0 24 8185.0 25 9413.0 26 10652.0 27 11904.0 28 13951.0 29 16393.0 30 20765.0 31 26627.0 32 35148.0 33 47727.0 34 66205.0 35 96070.0 36 165588.0 37 277650.0 38 788016.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.37678905891084 19.467022416981777 12.114358412552567 24.04183011155482 2 14.894502571722414 20.160867315371405 41.65395571939644 23.290674393509732 3 18.746989682781752 25.29449587417257 29.652669746562204 26.305844696483476 4 12.281446478857484 16.85684461026425 38.449228935935714 32.412479974942556 5 13.015071501775695 37.924873835112365 35.62191210223403 13.438142560877905 6 31.21370923135946 37.100374940863844 18.381340513619556 13.304575314157136 7 27.56957656219629 31.882704960035813 22.488322917709702 18.059395560058196 8 25.063844345219344 36.524853807195676 20.87611458948542 17.535187258099562 9 26.83534574738863 14.801939497517367 20.49484375910353 37.86787099599047 10 15.669589590437663 28.46279391034014 34.22868466076904 21.638931838453157 11 33.97260918813163 22.32789537945542 25.07603818826402 18.623457244148927 12 23.80901384781409 24.494814277829494 31.73899688962585 19.957174984730568 13 29.20270391156142 22.067917945724027 26.0507495433059 22.67862859940865 14 21.03993912827022 22.598806222051174 27.907781447892706 28.4534732017859 15 22.985448415676085 31.157683196471712 23.810446560357917 22.046421827494285 16 21.81588137469017 27.961654917832806 28.178814854800443 22.04364885267658 17 21.39366969232583 28.613639042791466 28.056760579983496 21.935930684899212 18 21.360422460644042 26.679550768315924 30.779174763300173 21.180852007739862 19 23.24668860258706 26.596196689866087 28.379024025885872 21.778090681660977 20 23.783098154242044 26.486109183627864 28.93520206427575 20.79559059785434 21 22.959034551787692 26.35428740797197 27.959113943330905 22.727564096909433 22 23.45108711624708 27.672295728535406 27.892187692868355 20.98442946234916 23 22.067237165972227 27.404914657532714 28.813490506029314 21.714357670465745 24 23.331381698534862 26.214833759590796 29.103233627178067 21.35055091469628 25 22.460144190966112 27.109197992993746 28.9929907987661 21.43766701727404 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 961.0 1 961.0 2 1373.0 3 1785.0 4 1785.0 5 1785.0 6 3226.0 7 4667.0 8 4667.0 9 4667.0 10 5242.0 11 5817.0 12 5817.0 13 5817.0 14 7497.5 15 9178.0 16 9178.0 17 9178.0 18 15129.5 19 21081.0 20 21081.0 21 21081.0 22 32908.0 23 44735.0 24 44735.0 25 44735.0 26 65272.0 27 85809.0 28 85809.0 29 85809.0 30 109114.0 31 132419.0 32 132419.0 33 132419.0 34 147601.0 35 162783.0 36 162783.0 37 162783.0 38 172785.5 39 182788.0 40 182788.0 41 182788.0 42 192250.0 43 201712.0 44 201712.0 45 201712.0 46 211296.5 47 220881.0 48 220881.0 49 220881.0 50 212903.5 51 204926.0 52 204926.0 53 204926.0 54 182125.0 55 159324.0 56 159324.0 57 159324.0 58 137468.0 59 115612.0 60 115612.0 61 115612.0 62 96120.5 63 76629.0 64 76629.0 65 76629.0 66 58816.0 67 41003.0 68 41003.0 69 41003.0 70 30161.5 71 19320.0 72 19320.0 73 19320.0 74 13563.0 75 7806.0 76 7806.0 77 7806.0 78 5390.0 79 2974.0 80 2974.0 81 2974.0 82 1999.0 83 1024.0 84 1024.0 85 1024.0 86 707.0 87 390.0 88 390.0 89 390.0 90 276.0 91 162.0 92 162.0 93 162.0 94 109.5 95 57.0 96 57.0 97 57.0 98 48.0 99 39.0 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3557758107662385 2 0.30606579563608277 3 0.32607891861056104 4 0.31604301236822735 5 0.30007946559679605 6 0.259055497974625 7 0.3587102862756928 8 0.3293068416709608 9 0.27460821817473274 10 0.24731759593680783 11 0.25864467140330144 12 0.25870336091349044 13 0.2766623510313508 14 0.2682697510743115 15 0.35565843174586037 16 0.409418023079063 17 0.4273770131969233 18 0.11990266931630242 19 0.13257960351714498 20 0.1956708269704123 21 0.20242012064215714 22 0.15212321041011057 23 0.2602879776885958 24 0.3615273827647689 25 0.4006732860608892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1703882.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.66342064830117 #Duplication Level Percentage of deduplicated Percentage of total 1 82.64188859915997 55.091909828558464 2 11.508813651032613 15.344337711633955 3 3.0185723524799566 6.0368507547211 4 1.136046548237127 3.0293099568472863 5 0.5271596093552403 1.757113139362125 6 0.2583895337078521 1.0335078106010964 7 0.17610613244160334 0.8217886029990028 8 0.1237088174376257 0.6597482347798669 9 0.09333837571955672 0.560002986289979 >10 0.4479621310757741 5.27527421902301 >50 0.03572908675057068 1.6839263708156749 >100 0.02653620020916682 3.614548615693932 >500 0.0035378230110825115 1.582685986272466 >1k 0.0019458026560953813 2.351358534163318 >5k 2.6533672583118836E-4 1.157637248238781 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8928 0.5239799469681586 No Hit GGTATCAACGCAGAGTACTTTTTTT 5543 0.32531595497810295 No Hit TATCAACGCAGAGTACTTTTTTTTT 5163 0.30301394110625035 No Hit GTCCTACAGTGGACATTTCTAAATT 3497 0.20523721713123327 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3330 0.1954360689296559 No Hit CTGTAGGACGTGGAATATGGCAAGA 3282 0.1926189724405798 No Hit CTTTAGGACGTGAAATATGGCGAGG 2975 0.17460129281253045 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2547 0.1494821824516017 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2503 0.14689984400328193 No Hit GTACTGGTTCACTATCGGTCAGTCA 2244 0.13169926086430866 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1957 0.11485537144004104 No Hit GTCCTACAGTGTGCATTTCTCATTT 1864 0.10939724699245605 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.8689510189085864E-5 0.0 5 0.0 0.0 0.0 5.8689510189085864E-5 0.0 6 0.0 0.0 0.0 5.8689510189085864E-5 0.0 7 0.0 0.0 0.0 5.8689510189085864E-5 0.0 8 0.0 0.0 0.0 5.8689510189085864E-5 0.0 9 0.0 0.0 0.0 5.8689510189085864E-5 0.0 10 0.0 0.0 0.0 1.1737902037817173E-4 0.0 11 0.0 0.0 0.0 1.1737902037817173E-4 0.0 12 0.0 0.0 0.0 1.760685305672576E-4 0.0 13 0.0 0.0 0.0 1.760685305672576E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1420 0.0 14.183472 1 TAGGACC 535 0.0 13.318501 4 TCCTATA 170 1.8189894E-12 11.736642 2 TTAGAAC 130 3.2603566E-8 10.962798 3 ATTATAC 245 0.0 10.85839 3 GACGTGA 975 0.0 10.816947 7 TTAGGAC 1105 0.0 10.747841 3 GTTCTAA 195 1.8189894E-12 10.718231 1 TGTAGGA 1785 0.0 10.698708 2 CGTGCGC 80 3.800039E-4 10.678648 10 GTATTAT 170 1.382432E-10 10.617928 1 TGTTATA 225 0.0 10.556769 2 ATAGGAC 180 3.45608E-11 10.556768 3 AGGACCT 1390 0.0 10.525768 5 GTATCAA 3140 0.0 10.498685 1 GGCGAGG 845 0.0 10.461452 19 TGGCGAG 2045 0.0 10.454758 18 CTGTAGG 1770 0.0 10.359031 1 GTGGTAT 405 0.0 10.32126 1 GTAGGAC 1780 0.0 10.301745 3 >>END_MODULE