FastQCFastQC Report
Thu 26 May 2016
SRR1512312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512312_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703882
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT105110.6168854415974815No Hit
GGTATCAACGCAGAGTACTTTTTTT69130.4057205839371506No Hit
TATCAACGCAGAGTACTTTTTTTTT65750.38588352949323956No Hit
GTCCTACAGTGGACATTTCTAAATT37430.2196748366377484No Hit
CTGTAGGACGTGGAATATGGCAAGA36310.21310161149657078No Hit
CTTTAGGACGTGAAATATGGCGAGG34610.2031243947644262No Hit
GTCCTAAAGTGTGTATTTCTCATTT33320.1955534479500341No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31980.1876890535846966No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27420.16092663693847345No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22110.12976250702806885No Hit
GTACTGGTTCACTATCGGTCAGTCA21920.12864740633447622No Hit
GTCCTACAGTGTGCATTTCTCATTT20180.11843543156157528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.015.3783691
CCGAATG1058.913048E-1114.46722318
TAGGACC5850.012.6696034
GGCGAGG8850.012.44463119
GGCGTGC951.0344738E-612.0052518
TGCGCCA1302.6120688E-911.69295912
CGAATGG1251.8477294E-811.39327319
CTTAGAC1354.7148205E-911.2612093
TCCGAAT1354.774847E-911.25228517
TCCAACG1451.2296368E-911.13103318
GGACGGG600.00585694711.0881836
TGGCGAG17900.011.08530918
CCCGTTT951.359457E-511.00061112
GCGCCAC1408.347342E-910.85742913
TTAGGAC12650.010.8161023
TTAGGGT1158.790212E-710.741624
AGGACCG803.7630025E-410.6899785
GTGCGCC1252.2381937E-710.64059211
CCAACGA1252.2582026E-710.63372119
AGGACGT21200.010.5779245
Produced by FastQC (version 0.11.5)