FastQCFastQC Report
Thu 26 May 2016
SRR1512311_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512311_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750753
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT61700.352419787371491No Hit
GTCCTAAAGTGTGTATTTCTCATTT58440.3337992281035646No Hit
GTATCAACGCAGAGTACTTTTTTTT55540.3172349269143049No Hit
CTGTAGGACGTGGAATATGGCAAGA55510.317063572074416No Hit
CTTTAGGACGTGAAATATGGCGAGG47050.268741507225748No Hit
GGTATCAACGCAGAGTACTTTTTTT34640.19785772179170907No Hit
GTCCTACAGTGTGCATTTCTCATTT34590.19757213039189422No Hit
TATCAACGCAGAGTACTTTTTTTTT32380.18494899052007907No Hit
CTGAAGGACCTGGAATATGGCGAGA25520.14576585046548543No Hit
CTGTAGGACCTGGAATATGGCGAGA22740.12988696863578128No Hit
GTCCTTCAGTGTGCATTTCTCATTT21320.12177617288104033No Hit
ATTTAGAAATGTCCACTGTAGGACG21160.1208622804016329No Hit
TTTCTAAATTTTCCACCTTTTTCAG20320.11606434488474389No Hit
GAATATGGCAAGAAAACTGAAAATC18780.10726812977044735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.012.8805591
TAGGACC8950.012.3110264
GTCCTAG2850.011.3326311
TATGTCG1604.5474735E-1111.29449416
GTCGAGG1354.6875357E-911.26535319
TCCAACG2200.011.22883318
TGTCGAG1701.0913936E-1111.17802418
CTGAGCG600.00594448311.0661289
TAGAAAT7600.010.873394
GGCGAGG12600.010.86301919
GTCTAAT700.001492330310.856471
GACCGTG700.001493045210.8558467
GTTTAGG1951.8189894E-1210.7172851
GTCCTAC30150.010.680931
GTTCTAG2050.010.6578771
GACGTGG15450.010.574797
GATATAC4950.010.5549011
GGACGTG32100.010.5043746
TCCTACA31750.010.5017122
GTATCAA20000.010.496851
Produced by FastQC (version 0.11.5)