FastQCFastQC Report
Thu 26 May 2016
SRR1512311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512311_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750753
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT63820.3645288627236395No Hit
GTATCAACGCAGAGTACTTTTTTTT60690.3466508410952316No Hit
CTGTAGGACGTGGAATATGGCAAGA60250.34413763677686116No Hit
GTCCTAAAGTGTGTATTTCTCATTT56590.32323234631041614No Hit
CTTTAGGACGTGAAATATGGCGAGG54550.3115802171979714No Hit
GGTATCAACGCAGAGTACTTTTTTT39760.22710228113274689No Hit
TATCAACGCAGAGTACTTTTTTTTT38370.21916284021789484No Hit
GTCCTACAGTGTGCATTTCTCATTT34310.19597281855293125No Hit
CTGAAGGACCTGGAATATGGCGAGA30020.17146907644881945No Hit
CTGTAGGACCTGGAATATGGCGAGA25600.14622279670518915No Hit
ATTTAGAAATGTCCACTGTAGGACG22400.1279449471170405No Hit
GTCCTTCAGTGTGCATTTCTCATTT22290.12731664603744788No Hit
TTTCTAAATTTTCCACCTTTTTCAG22180.12668834495785528No Hit
GAATATGGCAAGAAAACTGAAAATC20690.11817772124337357No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18630.10641135557100288No Hit
GGAATATGGCGAGAAAACTGAAAAT17740.10132782865429904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC456.7458214E-414.7827153
TCCAACG1700.014.51807618
GGTATCA11950.013.6879941
TAGGACC9500.013.6053234
TAGAACG707.234492E-613.576744
GGCGAGG13650.012.37896619
TTAGTAC1001.4365469E-712.3541273
TGTCGAG1201.0131771E-811.86573518
CCAACGT1052.7470924E-711.75306319
TATGTCG1251.8524588E-811.39143116
GCCGGTT2200.011.2265311
CGACTAA855.3644442E-511.1680717
AAATGTC7950.011.1197827
AGGCACG600.005879054311.082610
CCACCTT7300.010.80034613
CCGGTTT2200.010.7947412
AATGTCC8800.010.6938238
GCGTGCG1252.2288987E-710.6438599
TGTCCAC8700.010.59125210
GTCCTAC30150.010.5666361
Produced by FastQC (version 0.11.5)