FastQCFastQC Report
Thu 26 May 2016
SRR1512310_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512310_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085850
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78630.37696862190473907No Hit
GTCCTAAAGTGTGTATTTCTCATTT72260.34642951314811704No Hit
CTGTAGGACGTGGAATATGGCAAGA71190.34129970995037995No Hit
CTTTAGGACGTGAAATATGGCGAGG60700.29100846177817197No Hit
GTATCAACGCAGAGTACTTTTTTTT55320.26521561953160583No Hit
GTCCTACAGTGTGCATTTCTCATTT47040.22551957235659323No Hit
GGTATCAACGCAGAGTACTTTTTTT34810.1668864012273174No Hit
CTGTAGGACCTGGAATATGGCGAGA34720.16645492245367596No Hit
CTGAAGGACCTGGAATATGGCGAGA32700.15677062108972362No Hit
TATCAACGCAGAGTACTTTTTTTTT32490.15576383728456025No Hit
GTCCTTCAGTGTGCATTTCTCATTT28700.13759378670565955No Hit
ATTTAGAAATGTCCACTGTAGGACG28590.1370664237600978No Hit
TTTCTAAATTTTCCACCTTTTTCAG24220.11611573219550782No Hit
GAATATGGCAAGAAAACTGAAAATC24070.11539660090610541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12850.014.0479394
GGTATCA12150.013.6051621
GGACCGT707.269542E-613.5706716
CCAACGA1750.013.03538319
TCCAACG2300.012.39240518
TAGAAAT9750.012.3754594
GGCGAGG17400.012.072419
AATGTCC9600.012.0498858
GGACCTG23850.011.8693586
AGGACCT25050.011.7193215
AAATGTC9700.011.6542697
ATTTAGA9450.011.5610371
TTTAGAC1406.8030204E-1011.5367383
GAAATGT10300.011.2517996
TTACCCT1104.96495E-711.228274
TTTAGAA11000.011.1418992
AGGACCG600.005873955311.084055
ATGTCCA10450.010.9829779
GTAAGAC2250.010.9787523
TAGGACT4100.010.8912114
Produced by FastQC (version 0.11.5)