FastQCFastQC Report
Thu 26 May 2016
SRR1512310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512310_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085850
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80830.3875158808159743No Hit
CTGTAGGACGTGGAATATGGCAAGA76050.36459956372701774No Hit
GTCCTAAAGTGTGTATTTCTCATTT74190.355682335738428No Hit
CTTTAGGACGTGAAATATGGCGAGG69250.3319989452741089No Hit
GTATCAACGCAGAGTACTTTTTTTT67560.3238967327468418No Hit
GTCCTACAGTGTGCATTTCTCATTT49180.2357791787520675No Hit
GGTATCAACGCAGAGTACTTTTTTT41890.20082939808711078No Hit
TATCAACGCAGAGTACTTTTTTTTT41590.19939113550830598No Hit
CTGTAGGACCTGGAATATGGCGAGA37260.17863221228755663No Hit
CTGAAGGACCTGGAATATGGCGAGA35750.17139295730757245No Hit
ATTTAGAAATGTCCACTGTAGGACG30310.14531246254524532No Hit
GTCCTTCAGTGTGCATTTCTCATTT29170.1398470647457871No Hit
TTTCTAAATTTTCCACCTTTTTCAG27930.13390224608672724No Hit
GAATATGGCAAGAAAACTGAAAATC25420.12186878251072705No Hit
GGAATATGGCGAGAAAACTGAAAAT22160.10623966248771485No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21210.10168516432149963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA651.9276013E-716.06706419
GGTATCA13000.014.7801261
CGGAAAT500.001506523613.29216314
TAGGACC13450.013.282454
AGCACCG957.357994E-813.00449855
ACCGAGC604.0753573E-412.673188
GCACCGC657.9909037E-411.6983196
GGCGAGG18050.011.67704619
AAATGTC10250.011.5913237
TAGAAAT10750.011.5799174
ACCTTTT9500.011.2930715
CACCTTT9350.011.27142814
AATGTCC10550.011.2617128
CCCGTTT1354.7439244E-911.25721412
TCCAACG1553.1650416E-1011.02549318
ATGTCCA11000.010.9730319
CCACCTT9450.010.85438813
TGTCCAC11050.010.8308710
GAAATGT10750.010.7869396
GTCCTAC44050.010.7751861
Produced by FastQC (version 0.11.5)