FastQCFastQC Report
Thu 26 May 2016
SRR1512309_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512309_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526591
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66540.2633588103495975No Hit
GTATCAACGCAGAGTACTTTTTTTT62570.24764593873721547No Hit
CTGTAGGACGTGGAATATGGCAAGA59870.23695960287992793No Hit
GTCCTAAAGTGTGTATTTCTCATTT54950.2174867242066484No Hit
CTTTAGGACGTGAAATATGGCGAGG47130.1865359292422082No Hit
GTCCTACAGTGTGCATTTCTCATTT38240.1513501789565466No Hit
GGTATCAACGCAGAGTACTTTTTTT36990.1464028012448394No Hit
TATCAACGCAGAGTACTTTTTTTTT35650.14109921233788927No Hit
CTGTAGGACCTGGAATATGGCGAGA28680.11351263421740994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11900.013.2526284
GGTATCA13550.012.7604011
GGACGTA907.4639265E-611.6113416
GAACGCT600.005836595311.09370417
TGTAGGA36800.011.075392
CGGGACT700.001491462510.8575754
GTAGGAC35350.010.8309153
GTCCTAC31550.010.6594831
CGCCCTA1252.214274E-710.64953216
CTGTAGG35050.010.5979281
GGCGAGG13750.010.57637919
AGGACGT35000.010.4773515
GACGTGG19050.010.4730657
GTAGGGC2100.010.4053833
GTATCAA29250.010.360891
TTAGACT2400.010.2920764
GTGTAGG4800.010.2918711
TCCTACA34950.010.2752912
GGACCTG22600.010.2148066
GGACGTG34700.010.1846416
Produced by FastQC (version 0.11.5)