FastQCFastQC Report
Thu 26 May 2016
SRR1512309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512309_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526591
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74850.296248977377027No Hit
GTCCTACAGTGGACATTTCTAAATT67460.26700008034541406No Hit
CTGTAGGACGTGGAATATGGCAAGA65670.2599154354622493No Hit
GTCCTAAAGTGTGTATTTCTCATTT55930.22136546833262685No Hit
CTTTAGGACGTGAAATATGGCGAGG51540.20399027780911116No Hit
GGTATCAACGCAGAGTACTTTTTTT46390.1836070816368775No Hit
TATCAACGCAGAGTACTTTTTTTTT45740.18103444522678977No Hit
GTCCTACAGTGTGCATTTCTCATTT40390.15985966862068296No Hit
CTGTAGGACCTGGAATATGGCGAGA32160.12728613376680278No Hit
CTGAAGGACCTGGAATATGGCGAGA28090.11117747193748415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG653.3318956E-614.6331411
GGTATCA13600.013.7078091
TAGGACC12200.013.2422344
CGAGCGA1001.9340623E-611.398478510
CCGGTTT1001.9345225E-611.39825312
AGGACCT21250.011.3151295
TGTAGGA36000.011.2978592
GCCGGTT1201.2787314E-711.08185511
CGGCAAA600.005908415711.07549517
GTAGGAC35300.010.8221843
CTGTAGG36350.010.702121
CGGTTTC1355.711445E-810.553102513
GGACCTG22350.010.5472926
AGGACGT35000.010.3184275
GGCGAGG13750.010.28787419
GGACGTG34000.010.2321996
TCCCGCC1951.4551915E-1110.22839213
GTATCAA28650.010.2253231
AATGTCC7650.010.188718
GTCCTAC34400.010.17513751
Produced by FastQC (version 0.11.5)