FastQCFastQC Report
Thu 26 May 2016
SRR1512308_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512308_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460765
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66390.2697941493803756No Hit
GTATCAACGCAGAGTACTTTTTTTT65620.2666650411559007No Hit
CTGTAGGACGTGGAATATGGCAAGA54700.22228859724516564No Hit
GTCCTAAAGTGTGTATTTCTCATTT51300.20847175573449722No Hit
CTTTAGGACGTGAAATATGGCGAGG42400.17230414119186513No Hit
GTCCTACAGTGTGCATTTCTCATTT39470.16039727483120086No Hit
GGTATCAACGCAGAGTACTTTTTTT38780.15759326875991816No Hit
TATCAACGCAGAGTACTTTTTTTTT37130.15088803685032906No Hit
CTGTAGGACCTGGAATATGGCGAGA27710.1126072583119477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11700.013.6422874
GTCGTAA500.001500086513.2998711
GGTATCA13200.013.0263891
GACGTTA550.00306718912.0920277
AGGACCG1201.0009899E-811.8760985
CGTCGGT1001.9145646E-611.40804619
TCTAGGA5200.011.32797052
CTAGGAC4850.011.165983
TGTAGGA35400.011.1648742
ACGGTGC600.005958617711.0628888
TTTAGAA8500.010.9539552
GGCGAGG12750.010.88610919
GTCCTAC30850.010.8394411
GTAGGAC34850.010.8230953
GTATTAG2550.010.8038171
GTGTAGG6000.010.7665621
GTATCAA29850.010.5660611
TCCTACA34650.010.4742732
GTCGAGG1553.623427E-910.42670819
GACGTGG17500.010.369537
Produced by FastQC (version 0.11.5)