FastQCFastQC Report
Thu 26 May 2016
SRR1512308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512308_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460765
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78730.3199411565102722No Hit
GTCCTACAGTGGACATTTCTAAATT66210.26906266953569313No Hit
CTGTAGGACGTGGAATATGGCAAGA61420.2495971781133103No Hit
GTCCTAAAGTGTGTATTTCTCATTT52590.2137140279547214No Hit
GGTATCAACGCAGAGTACTTTTTTT48930.1988406044461783No Hit
TATCAACGCAGAGTACTTTTTTTTT48550.19729636921851537No Hit
CTTTAGGACGTGAAATATGGCGAGG46830.19030667292488312No Hit
GTCCTACAGTGTGCATTTCTCATTT40790.16576146035887215No Hit
CTGTAGGACCTGGAATATGGCGAGA31130.1265053753609142No Hit
CTGAAGGACCTGGAATATGGCGAGA27350.11114429862258282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12750.013.3414114
GGTATCA16400.013.2203071
TCCAACG2350.012.11837118
TGTAGGA34400.011.6296022
CCAACGA1850.011.28887519
CTGTAGG33900.011.2765671
AGGACCT21500.011.1388065
GTATTAT2650.011.1241391
GTAGGAC36400.010.9645083
AGGACGT31350.010.791685
GGCGAGG13250.010.746719
GGACGTG30800.010.6475826
CCTACAC4650.010.6265343
GACTGCG909.458033E-510.56228357
TTAAGAC3350.010.49537853
GGACCTG21750.010.4890136
TAAGACC2900.010.4860254
AATCCCG2950.010.29740219
TGGCGAG30850.010.27737518
TAGGACG33450.010.2274014
Produced by FastQC (version 0.11.5)