FastQCFastQC Report
Thu 26 May 2016
SRR1512307_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512307_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818417
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70070.38533515689745534No Hit
GTCCTACAGTGGACATTTCTAAATT69840.38407032050404283No Hit
GTCCTAAAGTGTGTATTTCTCATTT67700.37230184275663947No Hit
CTGTAGGACGTGGAATATGGCAAGA65930.3625681018160301No Hit
CTTTAGGACGTGAAATATGGCGAGG57790.31780389206656123No Hit
GGTATCAACGCAGAGTACTTTTTTT45340.24933774816227522No Hit
TATCAACGCAGAGTACTTTTTTTTT41860.23020022360107723No Hit
GTCCTACAGTGTGCATTTCTCATTT31060.17080790599735923No Hit
CTGAAGGACCTGGAATATGGCGAGA30740.16904813362391574No Hit
GTCCTTCAGTGTGCATTTCTCATTT26320.14474127771572748No Hit
ATTTAGAAATGTCCACTGTAGGACG25770.14171666894887147No Hit
TTTCTAAATTTTCCACCTTTTTCAG23360.12846338326137516No Hit
GAATATGGCAAGAAAACTGAAAATC21660.11911459252745657No Hit
CTGTAGGACCTGGAATATGGCGAGA20810.11444019716049729No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20140.11075567375359997No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20020.11009575911355865No Hit
GGAATATGGCGAGAAAACTGAAAAT18870.1037715771464961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.013.6137391
AGTGCGT500.001519215313.2772178
CTAAGAC1551.8189894E-1212.2587063
TAGGACC10500.012.2149254
TCCGGTT550.003079659312.08519812
TAGAAAT10050.012.0056054
GGCGAGG14750.011.72977819
GTCCTAT2450.011.6332621
CCACCTT9300.011.54648713
GTCCTAC31750.011.4305191
GGATTAG752.0731667E-411.4005961
AAATGTC10050.011.3435647
AATGTCC9900.011.3038358
ATTTAGA9700.011.2634761
AAGGCGA855.273179E-511.18694817
TCCTACA35500.011.10621552
ATAATAC1553.1286618E-1011.0328353
TTTAGAA10650.010.9724052
ACCTTTT9850.010.90748715
GAAATGT10300.010.8837646
Produced by FastQC (version 0.11.5)