##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512307_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1818417 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.01298547032941 31.0 30.0 34.0 16.0 34.0 2 29.92096752285092 31.0 30.0 34.0 23.0 34.0 3 30.605059235587877 33.0 31.0 34.0 26.0 34.0 4 34.26900980358191 37.0 35.0 37.0 31.0 37.0 5 34.444462958716294 37.0 35.0 37.0 32.0 37.0 6 34.600361743208516 37.0 35.0 37.0 32.0 37.0 7 34.53964189732058 37.0 35.0 37.0 32.0 37.0 8 34.53576379895261 37.0 35.0 37.0 32.0 37.0 9 36.121645915100885 39.0 37.0 39.0 32.0 39.0 10 36.04561274999079 39.0 37.0 39.0 32.0 39.0 11 36.07097986875398 39.0 37.0 39.0 32.0 39.0 12 36.01651436386703 39.0 37.0 39.0 32.0 39.0 13 35.9961933923847 39.0 37.0 39.0 32.0 39.0 14 37.216012608769056 40.0 38.0 41.0 32.0 41.0 15 37.15527351537079 40.0 38.0 41.0 31.0 41.0 16 37.08698224884611 40.0 38.0 41.0 31.0 41.0 17 37.055997056780704 40.0 38.0 41.0 31.0 41.0 18 36.84130592707833 40.0 38.0 41.0 30.0 41.0 19 36.94580231047114 40.0 38.0 41.0 31.0 41.0 20 36.889254774894866 40.0 38.0 41.0 31.0 41.0 21 36.83032164789484 40.0 38.0 41.0 31.0 41.0 22 36.76320667921604 40.0 38.0 41.0 31.0 41.0 23 36.640215088178344 40.0 38.0 41.0 30.0 41.0 24 36.518425091714384 40.0 37.0 41.0 30.0 41.0 25 36.409058538278074 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9109097499702159 1101 2 0.08162942028114628 1101 3 -0.3657687079763221 1101 4 -0.4886293791854115 1101 5 -0.7880723236252436 1101 6 -0.7876940570821347 1101 7 -0.8662359324933746 1101 8 -0.9722033019448801 1101 9 -0.9999971316268699 1101 10 -1.012658992246827 1101 11 -1.153355233401534 1101 12 -1.2883303622107434 1101 13 -1.2696100619576924 1101 14 -1.5319314646181326 1101 15 -1.54704327255957 1101 16 -1.7355264201962797 1101 17 -1.8256990076710409 1101 18 -1.6042793677819844 1101 19 -1.7432853563576671 1101 20 -1.702622243386692 1101 21 -1.6715148547074534 1101 22 -1.956743694790724 1101 23 -1.9573048999379452 1101 24 -2.260888673612861 1101 25 -2.2137162939331105 1102 1 0.867393410875426 1102 2 -0.059340494095927454 1102 3 -0.5713075363300959 1102 4 -0.7284033734436974 1102 5 -0.9121406424247667 1102 6 -0.8632770703842993 1102 7 -0.7490249884148952 1102 8 -1.0399439806443596 1102 9 -1.0158972495850591 1102 10 -1.1229353591345088 1102 11 -1.01325968187966 1102 12 -1.232991990967406 1102 13 -1.3126660588839556 1102 14 -1.4972829961894547 1102 15 -1.4166849274214428 1102 16 -1.7998163280380766 1102 17 -1.8466891024146292 1102 18 -1.5903753825186797 1102 19 -1.7242170644892454 1102 20 -1.8298479636587999 1102 21 -1.9354305998818546 1102 22 -1.8142580984375556 1102 23 -2.167886306351491 1102 24 -1.9974458384509077 1102 25 -2.0363678576422046 1103 1 0.8791448475250441 1103 2 0.008444870961078266 1103 3 -0.5210048957527817 1103 4 -0.5898286931811754 1103 5 -0.5960409802798452 1103 6 -0.718892507605517 1103 7 -0.8495537163037596 1103 8 -0.8525007576497998 1103 9 -1.0352703293739793 1103 10 -1.1714578811052974 1103 11 -1.0956514100441126 1103 12 -1.131815112095083 1103 13 -1.0874963658115178 1103 14 -1.351553137353676 1103 15 -1.4256906536057201 1103 16 -1.5802207334233458 1103 17 -1.5393736723383782 1103 18 -1.5748718151364969 1103 19 -1.8858393812972807 1103 20 -1.8289966913435265 1103 21 -1.7061688795768646 1103 22 -1.9485845828053812 1103 23 -1.9834796962321732 1103 24 -1.9495061420101223 1103 25 -1.9309540106874934 1104 1 1.0548162972467061 1104 2 0.3525696818076405 1104 3 -0.16295224224000648 1104 4 -0.2327066778473963 1104 5 -0.4054148783159164 1104 6 -0.4379578655561076 1104 7 -0.45696081113595 1104 8 -0.5517492468756586 1104 9 -0.7114383375335009 1104 10 -0.7396345695511855 1104 11 -0.7031236004818027 1104 12 -0.6438015355724218 1104 13 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10 -0.32778911926208565 2314 11 -1.4246125304737376 2314 12 -1.021051663535161 2314 13 -0.7169821734395612 2314 14 -0.4959441424376294 2314 15 -0.502470403516007 2314 16 -0.636205409569456 2314 17 -1.022953517725071 2314 18 -1.9038424074311635 2314 19 -1.6853446251923927 2314 20 -1.8735426020146377 2314 21 -1.399599789357623 2314 22 -0.9530041550343071 2314 23 -0.7144838708890333 2314 24 -0.6830688027273766 2314 25 -0.5670035501654169 2315 1 -2.6004146244809796 2315 2 -1.2349839372093179 2315 3 -0.505920262172733 2315 4 -0.3655763946666042 2315 5 -0.12082777797739652 2315 6 -0.2353372385456396 2315 7 -0.2315023533217584 2315 8 -0.11380298954503587 2315 9 -0.2456406856113702 2315 10 -0.2867745125884156 2315 11 -0.2779203558959793 2315 12 -0.22783189844032137 2315 13 -0.18830915345235155 2315 14 -0.26967404973641607 2315 15 -0.8674463239715422 2315 16 -0.5749420656464324 2315 17 -0.4649730759670305 2315 18 -0.36947871565450896 2315 19 -0.3480101402942992 2315 20 -0.2732606374520543 2315 21 -0.4556979106596799 2315 22 -0.38071645137654286 2315 23 -0.33602906108851016 2315 24 -0.19014684144723049 2315 25 -0.04573209994604355 2316 1 1.4429108922659815 2316 2 0.6514482400047541 2316 3 0.08688650476211635 2316 4 -0.0032560236821694843 2316 5 -0.1959751992439962 2316 6 -0.33237105078794826 2316 7 -0.35921083712323565 2316 8 -0.3677982000992088 2316 9 -0.3930749230531916 2316 10 -0.46432872728090047 2316 11 -0.6228265301548319 2316 12 -0.5856135423772386 2316 13 -0.5583708290503324 2316 14 -0.6126996042819428 2316 15 -0.6319832442568583 2316 16 -0.5787831139103332 2316 17 -0.5289622146404582 2316 18 -0.47740483189859617 2316 19 -0.5603593294410913 2316 20 -0.521412254099836 2316 21 -0.44858607345261703 2316 22 -0.5927876066773692 2316 23 -0.6289250521670127 2316 24 -0.7522128618200057 2316 25 -0.7188421221506047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48205.0 3 1.0 4 402.0 5 1969.0 6 2382.0 7 2179.0 8 2035.0 9 1900.0 10 1924.0 11 1840.0 12 2008.0 13 1989.0 14 1931.0 15 1991.0 16 2058.0 17 2308.0 18 2462.0 19 3086.0 20 3746.0 21 4487.0 22 5754.0 23 6789.0 24 7894.0 25 9482.0 26 10445.0 27 11797.0 28 13736.0 29 16540.0 30 21066.0 31 27808.0 32 36044.0 33 49399.0 34 69213.0 35 99813.0 36 172968.0 37 293036.0 38 877730.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.742670695197496 19.635114440627458 12.076542085730448 24.5456727784446 2 14.147272391321733 20.073081876521965 43.181571232767155 22.598074499389146 3 18.735849431329193 25.208589864110408 29.804793870624636 26.250766833935767 4 12.007402851366521 16.981159136481107 38.95637447836652 32.055063533785855 5 12.491589028342492 38.3966647326028 36.23049481884146 12.881251420213246 6 30.983570496468605 37.45023764093477 18.568078986873928 12.998112875722695 7 27.557830912363258 31.615225536832796 22.82428663755873 18.002656913245215 8 24.204834710880583 38.33233479087432 20.854035159728177 16.608795338516927 9 26.782433597773327 14.744514358937035 20.66954705554122 37.80350498774842 10 15.527387084949162 28.452856398680325 35.02272495500486 20.99703156136565 11 33.68287110546558 21.600276542817827 26.424338855368546 18.292513496348047 12 24.44244875997429 24.070325274516875 32.49167270333654 18.995553262172297 13 29.614296663486932 22.361860860071573 25.8385945951907 22.185247881250792 14 20.25050200747856 23.298770812221083 27.910788777527706 28.53993840277265 15 22.086239303692896 33.34764474298511 23.20677853111959 21.359337422202405 16 20.929882004759566 27.78034222484802 30.01965667084463 21.270119099547784 17 20.779719947603382 29.442391335561442 28.788151561405872 20.9897371554293 18 20.687297180931953 26.636289458528832 32.427431103456854 20.248982257082357 19 23.675011673905956 26.239911983154336 28.09308948819834 21.99198685474137 20 23.879542714575695 27.166750051242943 28.99469056975542 19.959016664425935 21 22.260058098273362 26.48601321537356 27.68957977933236 23.564348907020722 22 23.334348551881614 28.96512145997606 27.730832334178366 19.96969765396395 23 21.08058755487238 28.683051836136542 29.24128630453744 20.995074304453638 24 23.366506238062364 26.406813166072578 29.82940043189345 20.397280163971608 25 22.10226679865137 27.79038061171929 29.350899150399073 20.756453439230263 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 974.0 1 974.0 2 1419.5 3 1865.0 4 1865.0 5 1865.0 6 2947.0 7 4029.0 8 4029.0 9 4029.0 10 4619.0 11 5209.0 12 5209.0 13 5209.0 14 6837.0 15 8465.0 16 8465.0 17 8465.0 18 15016.5 19 21568.0 20 21568.0 21 21568.0 22 35753.5 23 49939.0 24 49939.0 25 49939.0 26 74429.5 27 98920.0 28 98920.0 29 98920.0 30 128913.0 31 158906.0 32 158906.0 33 158906.0 34 175581.5 35 192257.0 36 192257.0 37 192257.0 38 199300.5 39 206344.0 40 206344.0 41 206344.0 42 214398.5 43 222453.0 44 222453.0 45 222453.0 46 229383.0 47 236313.0 48 236313.0 49 236313.0 50 223039.5 51 209766.0 52 209766.0 53 209766.0 54 181684.0 55 153602.0 56 153602.0 57 153602.0 58 131335.0 59 109068.0 60 109068.0 61 109068.0 62 89779.0 63 70490.0 64 70490.0 65 70490.0 66 54479.0 67 38468.0 68 38468.0 69 38468.0 70 28275.0 71 18082.0 72 18082.0 73 18082.0 74 12771.0 75 7460.0 76 7460.0 77 7460.0 78 5087.5 79 2715.0 80 2715.0 81 2715.0 82 1858.5 83 1002.0 84 1002.0 85 1002.0 86 650.0 87 298.0 88 298.0 89 298.0 90 209.0 91 120.0 92 120.0 93 120.0 94 90.5 95 61.0 96 61.0 97 61.0 98 52.0 99 43.0 100 43.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3487648872618327 2 0.29597171605852785 3 0.3172539631998601 4 0.3080701511259519 5 0.2919572353316099 6 0.24999766280231653 7 0.3596534788225143 8 0.3175839205198807 9 0.26248104807643136 10 0.23822918505491314 11 0.25258232847581163 12 0.2476329686755018 13 0.26655052169001936 14 0.25841157446284324 15 0.3488198801485028 16 0.40293288063189026 17 0.4186608462195415 18 0.11784975613404407 19 0.13203792089493224 20 0.19428986860549588 21 0.19582966943225893 22 0.14936068019601664 23 0.2564318305427193 24 0.3517345031420186 25 0.39022952381109505 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1818417.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.66376660468534 #Duplication Level Percentage of deduplicated Percentage of total 1 83.40506514528386 54.76690731348496 2 11.068777255829987 14.536352126521395 3 2.8129302526163262 5.5412278674917115 4 1.0263456510817026 2.6957488515345096 5 0.49090479687289923 1.6117329003491263 6 0.2828101376929819 1.1142227324946532 7 0.17789170754734634 0.8176727695708523 8 0.11962582096663536 0.6284065590277615 9 0.08849353401284235 0.5229736887098757 >10 0.45643916516546607 5.4781600641017265 >50 0.03393504457674024 1.5292659741425911 >100 0.029796467777803145 3.9971374036322977 >500 0.0038712162225262416 1.6914708970045638 >1k 0.0026930199808878207 3.237772267189206 >5k 4.207843720137219E-4 1.8309485847447553 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7007 0.38533515689745534 No Hit GTCCTACAGTGGACATTTCTAAATT 6984 0.38407032050404283 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6770 0.37230184275663947 No Hit CTGTAGGACGTGGAATATGGCAAGA 6593 0.3625681018160301 No Hit CTTTAGGACGTGAAATATGGCGAGG 5779 0.31780389206656123 No Hit GGTATCAACGCAGAGTACTTTTTTT 4534 0.24933774816227522 No Hit TATCAACGCAGAGTACTTTTTTTTT 4186 0.23020022360107723 No Hit GTCCTACAGTGTGCATTTCTCATTT 3106 0.17080790599735923 No Hit CTGAAGGACCTGGAATATGGCGAGA 3074 0.16904813362391574 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2632 0.14474127771572748 No Hit ATTTAGAAATGTCCACTGTAGGACG 2577 0.14171666894887147 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2336 0.12846338326137516 No Hit GAATATGGCAAGAAAACTGAAAATC 2166 0.11911459252745657 No Hit CTGTAGGACCTGGAATATGGCGAGA 2081 0.11444019716049729 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2014 0.11075567375359997 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2002 0.11009575911355865 No Hit GGAATATGGCGAGAAAACTGAAAAT 1887 0.1037715771464961 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.499288667010922E-5 2 0.0 0.0 0.0 0.0 5.499288667010922E-5 3 0.0 0.0 0.0 0.0 5.499288667010922E-5 4 0.0 0.0 0.0 0.0 5.499288667010922E-5 5 0.0 0.0 0.0 0.0 5.499288667010922E-5 6 0.0 0.0 0.0 0.0 5.499288667010922E-5 7 0.0 0.0 0.0 0.0 5.499288667010922E-5 8 0.0 0.0 0.0 0.0 5.499288667010922E-5 9 5.499288667010922E-5 0.0 0.0 0.0 5.499288667010922E-5 10 5.499288667010922E-5 0.0 0.0 0.0 5.499288667010922E-5 11 5.499288667010922E-5 0.0 0.0 0.0 5.499288667010922E-5 12 5.499288667010922E-5 0.0 0.0 0.0 5.499288667010922E-5 13 5.499288667010922E-5 0.0 0.0 0.0 5.499288667010922E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1305 0.0 13.613739 1 AGTGCGT 50 0.0015192153 13.277217 8 CTAAGAC 155 1.8189894E-12 12.258706 3 TAGGACC 1050 0.0 12.214925 4 TCCGGTT 55 0.0030796593 12.085198 12 TAGAAAT 1005 0.0 12.005605 4 GGCGAGG 1475 0.0 11.729778 19 GTCCTAT 245 0.0 11.633262 1 CCACCTT 930 0.0 11.546487 13 GTCCTAC 3175 0.0 11.430519 1 GGATTAG 75 2.0731667E-4 11.400596 1 AAATGTC 1005 0.0 11.343564 7 AATGTCC 990 0.0 11.303835 8 ATTTAGA 970 0.0 11.263476 1 AAGGCGA 85 5.273179E-5 11.186948 17 TCCTACA 3550 0.0 11.1062155 2 ATAATAC 155 3.1286618E-10 11.032835 3 TTTAGAA 1065 0.0 10.972405 2 ACCTTTT 985 0.0 10.907487 15 GAAATGT 1030 0.0 10.883764 6 >>END_MODULE