FastQCFastQC Report
Thu 26 May 2016
SRR1512307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512307_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818417
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82680.454681186988463No Hit
CTGTAGGACGTGGAATATGGCAAGA71470.3930341610312706No Hit
GTCCTACAGTGGACATTTCTAAATT70850.3896246020577238No Hit
GTCCTAAAGTGTGTATTTCTCATTT68540.3769212452369286No Hit
CTTTAGGACGTGAAATATGGCGAGG62940.34612522870166745No Hit
GGTATCAACGCAGAGTACTTTTTTT54590.3002061683321262No Hit
TATCAACGCAGAGTACTTTTTTTTT53610.29481686543845553No Hit
CTGAAGGACCTGGAATATGGCGAGA33670.18516104941825776No Hit
GTCCTACAGTGTGCATTTCTCATTT31880.1753173227043082No Hit
ATTTAGAAATGTCCACTGTAGGACG28760.1581595420632341No Hit
TTTCTAAATTTTCCACCTTTTTCAG26530.14589612833579976No Hit
GTCCTTCAGTGTGCATTTCTCATTT26350.14490625637573779No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25320.13924198904871654No Hit
GAATATGGCAAGAAAACTGAAAATC22840.12560375315452946No Hit
CTGTAGGACCTGGAATATGGCGAGA22020.12109433644758051No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21310.11718984149400274No Hit
GGAATATGGCGAGAAAACTGAAAAT19940.10965581602019779No Hit
ATTCCAGGTCCTTCAGTGTGCATTT18950.10421152023985698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.013.2769761
TAGGACC9750.012.5723824
GGCGAGG15850.011.50064119
GTCTTAA1900.011.0106351
ACCTTTT8900.010.88078615
CTGACGT700.001488498310.8598634
CACCTTT8650.010.75770914
CCTAATA1252.2342465E-710.642082
GTATCAA28200.010.453361
GACGTGG19450.010.4090717
AGGACCT21400.010.346625
GTGTAGA2300.010.3360711
CCACCTT8850.010.30369813
CCCTTAG1201.5049791E-610.3016191
TAGAAAT8550.010.2247844
GTCCTAC33100.010.1986731
AAATGTC8500.010.1759387
GGACCTG20850.010.1657366
GACTAAC750.002626794210.1431311
GCACCGT750.002637015710.1383846
Produced by FastQC (version 0.11.5)