FastQCFastQC Report
Thu 26 May 2016
SRR1512306_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512306_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819248
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65250.3586646790322155No Hit
GTCCTACAGTGGACATTTCTAAATT65100.35784016252869316No Hit
CTGTAGGACGTGGAATATGGCAAGA60180.3307960212131606No Hit
GTCCTAAAGTGTGTATTTCTCATTT58470.321396533073006No Hit
CTTTAGGACGTGAAATATGGCGAGG46610.25620476152784005No Hit
GGTATCAACGCAGAGTACTTTTTTT39630.21783726023060077No Hit
GTCCTACAGTGTGCATTTCTCATTT38290.21047157946580125No Hit
TATCAACGCAGAGTACTTTTTTTTT37020.20349067306931215No Hit
CTGAAGGACCTGGAATATGGCGAGA27190.1494573582051485No Hit
CTGTAGGACCTGGAATATGGCGAGA25320.13917838579457006No Hit
GTCCTTCAGTGTGCATTTCTCATTT23740.1304934786241348No Hit
ATTTAGAAATGTCCACTGTAGGACG22160.12180857145369955No Hit
GAATATGGCAAGAAAACTGAAAATC19410.10669243555579007No Hit
TTTCTAAATTTTCCACCTTTTTCAG19410.10669243555579007No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18660.10256985303817841No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18380.10103075556493672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTA402.75981E-416.626281
TAGGACC9650.014.0791954
GGTATCA11450.013.0272051
GACGGGA604.0908976E-412.6672947
CTAAGAC1950.012.1814363
TAGAAAT8150.011.6576614
GTCGAAA907.3760857E-611.62324415
AAATGTC8150.011.4238777
ATGCCGG1501.7462298E-1011.40812719
CACCTTT7200.011.35907914
AATGTCC8550.011.3107728
GTATTAA2200.011.2281371
TGTCCAC8800.011.21605310
AGGACCT21650.011.1902395
GGCGAGG13000.011.18874119
TGTAGGA34250.011.1244072
GGACCTG20600.011.1146286
CGCGATA600.005869299611.0851062
GCGCGAT600.005872921611.08418751
ATAGGAC2150.011.0482793
Produced by FastQC (version 0.11.5)