FastQCFastQC Report
Thu 26 May 2016
SRR1512306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512306_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819248
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76890.42264715970554867No Hit
CTGTAGGACGTGGAATATGGCAAGA66760.3669648118343403No Hit
GTCCTACAGTGGACATTTCTAAATT65810.36174287397869886No Hit
GTCCTAAAGTGTGTATTTCTCATTT57880.31815343482581815No Hit
CTTTAGGACGTGAAATATGGCGAGG52580.289020518368029No Hit
GGTATCAACGCAGAGTACTTTTTTT48730.26785792811095577No Hit
TATCAACGCAGAGTACTTTTTTTTT48620.26725328267503934No Hit
GTCCTACAGTGTGCATTTCTCATTT37400.20557944821156873No Hit
CTGAAGGACCTGGAATATGGCGAGA29710.16330923546432372No Hit
CTGTAGGACCTGGAATATGGCGAGA27480.15105142344529168No Hit
ATTTAGAAATGTCCACTGTAGGACG23790.13076831745864226No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23650.12999876872202143No Hit
GTCCTTCAGTGTGCATTTCTCATTT22750.12505166970088738No Hit
TTTCTAAATTTTCCACCTTTTTCAG21800.11982973184524595No Hit
GAATATGGCAAGAAAACTGAAAATC20270.11141966350931815No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19860.10916598506635708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC456.768679E-414.77617410
TAGGACC10700.014.2091464
GGTATCA11300.013.6371171
CGAATAT500.001500945813.29855612
CGGTTTC1052.7254464E-711.76030413
TCTTACG658.011523E-411.6945762
GCCGGTT1157.086055E-811.56396211
GTATTAG1251.7973434E-811.4147731
GGCGAGG13750.011.32438419
GAAATGT9250.011.3019766
AATGTCC9100.011.1749578
AGGACCT21650.011.1043825
GACGTGG17050.011.0925697
AAATGTC9000.011.0879247
GGACCTG21100.011.0804186
GTATCAA26000.010.9025711
TAACCTG1408.3200575E-910.8598475
TGTAGGA35800.010.8289162
GTCTTAC1951.8189894E-1210.7318371
ATGTCCA9500.010.7038439
Produced by FastQC (version 0.11.5)