FastQCFastQC Report
Thu 26 May 2016
SRR1512305_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512305_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657250
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75640.45641876602805853No Hit
GGTATCAACGCAGAGTACTTTTTTT48570.2930758787147383No Hit
TATCAACGCAGAGTACTTTTTTTTT45530.27473223713984013No Hit
GTCCTACAGTGGACATTTCTAAATT43910.2649570070900588No Hit
GTCCTAAAGTGTGTATTTCTCATTT41210.24866495700709007No Hit
CTGTAGGACGTGGAATATGGCAAGA38920.23484688489968322No Hit
CTTTAGGACGTGAAATATGGCGAGG32970.1989440337909187No Hit
GTCCTACAGTGTGCATTTCTCATTT24460.14759390556645044No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21860.13190526474581385No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20770.1253281037863931No Hit
CTGAAGGACCTGGAATATGGCGAGA17600.10620003017046312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11950.013.8329011
TAGGACC7400.013.2244294
GGCGGTG550.003047296312.10215215
GTACTAG951.0396489E-612.000231
TGGACCG802.8633787E-511.87630755
GACTGCG658.0164656E-411.6935967
GGACGTA658.018506E-411.693246
AATGTCC5400.011.2376638
TATAGGA3300.011.228852
TCTACAC2050.011.1235143
TCGAACT1053.440131E-610.8639419
TGTAGGA22500.010.8526342
GTAAGAC1158.799925E-710.740643
CCTACAC2050.010.6600343
AGGACCT15350.010.6461245
GTGTAGG2950.010.6273221
GGACCTG14450.010.585626
AAATGTC5300.010.5766367
GGCGAGG10100.010.54124819
CCGAATG1106.0281036E-610.36603518
Produced by FastQC (version 0.11.5)