FastQCFastQC Report
Thu 26 May 2016
SRR1512305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657250
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84540.5101221903756223No Hit
TATCAACGCAGAGTACTTTTTTTTT55790.33664202745512145No Hit
GGTATCAACGCAGAGTACTTTTTTT55240.3333232765122945No Hit
GTCCTACAGTGGACATTTCTAAATT41850.252526776286016No Hit
CTGTAGGACGTGGAATATGGCAAGA41370.24963041182682155No Hit
GTCCTAAAGTGTGTATTTCTCATTT40660.24534620606426308No Hit
CTTTAGGACGTGAAATATGGCGAGG36870.2224769950218736No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25750.1553778850505355No Hit
GTCCTACAGTGTGCATTTCTCATTT24340.14686981445165184No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22720.13709458440187058No Hit
CTGAAGGACCTGGAATATGGCGAGA19990.12062151154020213No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18350.11072559963795443No Hit
CTGTAGGACCTGGAATATGGCGAGA17660.10656207572786242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG456.746788E-414.7823585
GGTATCA12650.014.7399571
TAGGACC7700.013.3284
CCGAATG802.0137286E-613.05432718
GGGCCGT600.00585712711.0881076
CCGTGCC600.005862396711.0867699
GCACCGT700.001486141510.8618216
TAGAAAT6200.010.7284834
CGAATGG909.578522E-510.54863319
GGCGAGG10100.010.52774419
AGGACCT15900.010.5189835
ATAGGAC2100.010.4073573
TGTAGGA25850.010.2923932
TCCAACG750.00266151210.12699318
GTATCAA26700.010.1190021
GGACCTG16000.010.0980996
TGGCGAG23900.010.08991718
TTCCGAA951.6543861E-49.99374316
GTCCTAT2105.456968E-129.9663021
GTCCTAC24450.09.9607431
Produced by FastQC (version 0.11.5)