FastQCFastQC Report
Thu 26 May 2016
SRR1512304_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512304_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655294
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67400.40717842268503357No Hit
GTCCTACAGTGGACATTTCTAAATT48730.2943887913567016No Hit
GTCCTAAAGTGTGTATTTCTCATTT45190.2730028623314046No Hit
CTGTAGGACGTGGAATATGGCAAGA42250.25524166703920875No Hit
GGTATCAACGCAGAGTACTTTTTTT39500.2386283040958283No Hit
TATCAACGCAGAGTACTTTTTTTTT37720.22787492735429474No Hit
CTTTAGGACGTGAAATATGGCGAGG37550.22684791946324945No Hit
GTCCTACAGTGTGCATTTCTCATTT21300.1286780475250922No Hit
CTGAAGGACCTGGAATATGGCGAGA20460.12360342029875054No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19840.11985786210787933No Hit
GTACTGGTTCACTATCGGTCAGTCA18830.11375622699049233No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17740.10717129404202518No Hit
GTCCTTCAGTGTGCATTTCTCATTT17330.10469439265773935No Hit
ATTTAGAAATGTCCACTGTAGGACG16710.10094883446686811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11750.014.4730811
ACTGCGG400.00534806714.222228
TAGGACC6600.012.9559964
CCAACGA751.4724035E-512.67193219
ACCGTGC604.160174E-412.6419748
GCATTAA1001.4416582E-712.3506321
GGGCCGT550.003066008712.0922626
GCCTAAT550.00306740412.0915281
TGTAGGA24350.011.3161212
GGCGAGG11350.010.71811119
GATATAC4550.010.6488971
TCCAACG1701.382432E-1010.616918
GTAGGAC24150.010.5836083
CTGTAGG23300.010.5198521
AATCCCG2001.8189894E-1210.45434519
TATCGCG1002.3956512E-510.4511697
CCCGCCC2650.010.40529214
TCCCGCC2650.010.39833713
AGGACGT25850.010.2912875
GTATCAA25350.010.2687691
Produced by FastQC (version 0.11.5)