FastQCFastQC Report
Thu 26 May 2016
SRR1512304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655294
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78900.4766524859028064No Hit
GTCCTACAGTGGACATTTCTAAATT49750.3005508387029736No Hit
GGTATCAACGCAGAGTACTTTTTTT49130.29680528051210237No Hit
TATCAACGCAGAGTACTTTTTTTTT48380.29227436334572593No Hit
CTGTAGGACGTGGAATATGGCAAGA45690.27602347377565556No Hit
GTCCTAAAGTGTGTATTTCTCATTT45080.27233832781366935No Hit
CTTTAGGACGTGAAATATGGCGAGG41410.2501670398128671No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24490.14794954853941356No Hit
GTCCTACAGTGTGCATTTCTCATTT22900.1383440041466954No Hit
CTGAAGGACCTGGAATATGGCGAGA22670.13695452288233992No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20900.12626155836969144No Hit
GTACTGGTTCACTATCGGTCAGTCA18380.11103767669066644No Hit
ATTTAGAAATGTCCACTGTAGGACG17970.10856077530638061No Hit
GTCCTTCAGTGTGCATTTCTCATTT17820.10765459187310532No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17710.1069900573553701No Hit
TTTCTAAATTTTCCACCTTTTTCAG17130.10348614808003895No Hit
CTGTAGGACCTGGAATATGGCGAGA16980.10257996464676365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.014.7771541
TAGGACC6300.014.1787774
GGTTAGG701.0806376E-412.2267571
TTCGTGA600.005880247311.0822410
GGCGAGG10950.010.92415419
TGTAGGA23900.010.6952932
AGGACGT26000.010.5261765
GTAGGAC24300.010.5192393
GGACGTG25800.010.4998176
TTAGGAC14450.010.4560493
ATAGGAC1553.6452548E-910.4221083
GACGTGG12500.010.4175857
GTGGTAT3850.010.3742171
TGGCGAG24150.010.26017618
AGGACCT15550.010.2055765
CTGTAGG24500.010.1695561
TGTAGAA6000.010.136012
TAGGACG27100.010.133984
CCCTTAT856.5318454E-410.0690941
TGGTATC3400.010.0614812
Produced by FastQC (version 0.11.5)