FastQCFastQC Report
Thu 26 May 2016
SRR1512303_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512303_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729123
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64570.37342629760867213No Hit
GTCCTACAGTGGACATTTCTAAATT55960.3236322690751323No Hit
GTCCTAAAGTGTGTATTTCTCATTT52140.30154014491739456No Hit
CTGTAGGACGTGGAATATGGCAAGA48850.2825131584045785No Hit
CTTTAGGACGTGAAATATGGCGAGG46610.26955861439585266No Hit
GGTATCAACGCAGAGTACTTTTTTT39470.22826600536803918No Hit
TATCAACGCAGAGTACTTTTTTTTT36580.21155233028535275No Hit
GTCCTACAGTGTGCATTTCTCATTT33050.19113735691445893No Hit
CTGTAGGACCTGGAATATGGCGAGA24580.14215298738146448No Hit
CTGAAGGACCTGGAATATGGCGAGA21920.1267694663711026No Hit
ATTTAGAAATGTCCACTGTAGGACG21560.12468748608398593No Hit
GTCCTTCAGTGTGCATTTCTCATTT20300.11740055507907766No Hit
TTTCTAAATTTTCCACCTTTTTCAG18860.10907263393061108No Hit
GAATATGGCAAGAAAACTGAAAATC18590.10751114871527358No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18150.104966506142131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10550.013.9582851
TAGGACC11250.013.5960754
AGCGGAC852.761044E-713.3869868
TAGAACG500.001499228913.30050854
GCGGACA701.1064271E-412.1959659
CCATTCG658.125868E-411.6747699
GGCGAGG13550.011.57476419
CCGGGGG1001.9047529E-611.41237716
TGTAGGA34650.011.4055882
GCGCAGA855.319746E-511.1772231
GTACCGT855.324473E-511.1762496
AGGACCT19350.011.0960325
AATGCCG1201.2715827E-711.0856918
GTCCTAA15000.011.0840791
CCCCGTA951.3449198E-511.01130315
TAGGACT3200.010.9847964
GGACCTG19000.010.9497816
TCCAACG1408.3218765E-910.85945118
TTAGGAC17850.010.8575593
ATTATAC1752.0008883E-1110.8575583
Produced by FastQC (version 0.11.5)