FastQCFastQC Report
Thu 26 May 2016
SRR1512303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512303_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729123
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73540.4253023064293286No Hit
GTCCTACAGTGGACATTTCTAAATT56690.32785406243511883No Hit
CTGTAGGACGTGGAATATGGCAAGA53650.3102728955661338No Hit
GTCCTAAAGTGTGTATTTCTCATTT51180.2959881974850835No Hit
CTTTAGGACGTGAAATATGGCGAGG51080.29540986962755106No Hit
GGTATCAACGCAGAGTACTTTTTTT49310.2851734665492276No Hit
TATCAACGCAGAGTACTTTTTTTTT44980.26013187031807455No Hit
GTCCTACAGTGTGCATTTCTCATTT34070.19703630106128944No Hit
CTGTAGGACCTGGAATATGGCGAGA26810.15504969860443704No Hit
CTGAAGGACCTGGAATATGGCGAGA25650.1483410954570612No Hit
ATTTAGAAATGTCCACTGTAGGACG23010.13307324001820578No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22450.1298346040160243No Hit
GTCCTTCAGTGTGCATTTCTCATTT20860.12063919108125912No Hit
TTTCTAAATTTTCCACCTTTTTCAG20650.11942470258044108No Hit
GAATATGGCAAGAAAACTGAAAATC19350.11190644043251985No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18730.10832080771581894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.013.9292551
TAGGACC9700.013.424
ACGTTTC500.001501181213.29821813
TGTCGAG802.0125644E-613.05508318
ATGTCGA1001.452936E-712.34334617
AAATGTC8150.012.244717
ATTTAGA8250.012.2199821
CCAACGA1103.844798E-812.08404419
GGCGAGG13400.012.04539619
AATGTCC8150.012.0114778
AATCCCG1750.011.93607619
ATCCCGT802.8673325E-511.87478310
CGTTTCC802.8818004E-511.86894414
TCCAACG1459.822543E-1111.78640718
CCCCCGG907.509123E-611.60451918
TAGAAAT8800.011.5532664
ATGTCCA9000.011.2984899
GAAATGT8600.011.2724546
TTTAGAA9550.011.2425842
CCCCGGT855.3543117E-511.17012519
Produced by FastQC (version 0.11.5)