FastQCFastQC Report
Thu 26 May 2016
SRR1512302_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512302_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946898
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62450.32076667601487086No Hit
GGTATCAACGCAGAGTACTTTTTTT41400.21264596296262056No Hit
TATCAACGCAGAGTACTTTTTTTTT38480.1976477452850637No Hit
GTCCTACAGTGGACATTTCTAAATT37290.19153545794386762No Hit
GTCCTAAAGTGTGTATTTCTCATTT35790.18383089406841036No Hit
CTGTAGGACGTGGAATATGGCAAGA34050.17489359997287993No Hit
CTTTAGGACGTGAAATATGGCGAGG29650.15229354593820527No Hit
GTCCTACAGTGTGCATTTCTCATTT23400.12019119645713336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.013.7002421
TAGGACC7650.013.1660824
CGCCCTA1001.9081854E-611.41087216
GGACCTG14300.011.2289826
GATTTAC600.00587440311.0838761
GTCTTAG2100.010.8576741
AGGACCT14700.010.7300795
TTAGGAC12350.010.6947643
TGTAGGA21950.010.6926612
GTAGGAC22500.010.4735173
AGGACGT20550.010.449825
GACGTGA11200.010.349797
ACCTTTT5050.010.16784115
GTATTAA2350.010.1068771
GTCCTAC20500.010.1029331
GGACGTG20800.010.0952876
ACGTGAA12600.010.0836168
TGGCGAG22650.010.06775818
CTGTAGG21250.010.0593161
GGCGAGG11450.010.0457619
Produced by FastQC (version 0.11.5)