##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512302_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1946898 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.930344578914767 31.0 30.0 34.0 16.0 34.0 2 29.825680133217045 31.0 30.0 34.0 22.0 34.0 3 30.48852328165112 33.0 31.0 34.0 26.0 34.0 4 34.15021845006775 37.0 35.0 37.0 30.0 37.0 5 34.31978973731546 37.0 35.0 37.0 31.0 37.0 6 34.473867146609635 37.0 35.0 37.0 32.0 37.0 7 34.40786009333822 37.0 35.0 37.0 32.0 37.0 8 34.4107318411134 37.0 35.0 37.0 32.0 37.0 9 35.9847757817821 39.0 37.0 39.0 32.0 39.0 10 35.91846105959326 39.0 37.0 39.0 31.0 39.0 11 35.94171754247012 39.0 37.0 39.0 31.0 39.0 12 35.87348746570185 39.0 37.0 39.0 31.0 39.0 13 35.849366017120566 39.0 37.0 39.0 31.0 39.0 14 37.07396227229162 40.0 38.0 41.0 31.0 41.0 15 37.006804157177214 40.0 38.0 41.0 31.0 41.0 16 36.93075137988739 40.0 38.0 41.0 31.0 41.0 17 36.88445516919736 40.0 38.0 41.0 31.0 41.0 18 36.66821322945527 40.0 37.0 41.0 30.0 41.0 19 36.778573916044905 40.0 38.0 41.0 30.0 41.0 20 36.7045186753492 40.0 38.0 41.0 30.0 41.0 21 36.648849092248284 40.0 38.0 41.0 30.0 41.0 22 36.57458017831443 40.0 37.0 41.0 30.0 41.0 23 36.45158195241867 40.0 37.0 41.0 30.0 41.0 24 36.323582950930145 40.0 37.0 41.0 30.0 41.0 25 36.20560450521804 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0530975880830447 1101 2 0.2187117766133433 1101 3 -0.17400847054031132 1101 4 -0.2951982051869777 1101 5 -0.5383560947562813 1101 6 -0.5407474324860502 1101 7 -0.7001606197559198 1101 8 -0.5892590661338986 1101 9 -0.7304113693178635 1101 10 -0.5929082519328546 1101 11 -0.7053091371141633 1101 12 -0.7644940396401054 1101 13 -0.9982861734485482 1101 14 -1.15902243565818 1101 15 -1.2431658766341158 1101 16 -1.20503413375895 1101 17 -1.2495065477417313 1101 18 -1.0864170453588073 1101 19 -1.1853011658348223 1101 20 -1.4368676674576761 1101 21 -1.476228486831566 1101 22 -1.3398050584524626 1101 23 -1.545425264396897 1101 24 -1.9011469827948702 1101 25 -1.776081039745634 1102 1 1.1610036336713137 1102 2 0.34468298490481786 1102 3 -0.1496960842146393 1102 4 -0.19519593020376647 1102 5 -0.2883869267655328 1102 6 -0.4040951780375579 1102 7 -0.2645947296567712 1102 8 -0.576607166103841 1102 9 -0.5418338205284599 1102 10 -0.598035944345547 1102 11 -0.6218974765081313 1102 12 -0.8368726303718219 1102 13 -0.7012440108255618 1102 14 -0.877052635461574 1102 15 -0.9040868137835147 1102 16 -1.0259821791293007 1102 17 -1.159812940085338 1102 18 -0.9587240339874867 1102 19 -0.9224807374435073 1102 20 -1.1657830632269253 1102 21 -1.1560377713972372 1102 22 -1.1916473757743091 1102 23 -1.328167361524315 1102 24 -1.1913348604869824 1102 25 -1.334568463279254 1103 1 1.0874788806500923 1103 2 0.20826804142985722 1103 3 -0.25847590749616955 1103 4 -0.23336264066648482 1103 5 -0.3824847572011052 1103 6 -0.4629596017837585 1103 7 -0.5943289092010673 1103 8 -0.6057398861424232 1103 9 -0.6930497658169656 1103 10 -0.8250099598769225 1103 11 -0.6553651614939255 1103 12 -0.9181805136046108 1103 13 -0.815597561428568 1103 14 -1.0132785150447532 1103 15 -0.9993862690431783 1103 16 -1.188062264568174 1103 17 -1.116732256506829 1103 18 -1.1073338798272658 1103 19 -1.288221178603841 1103 20 -1.2799357334449155 1103 21 -1.2988879208196096 1103 22 -1.4175463717662637 1103 23 -1.564535770523527 1103 24 -1.394956390587815 1103 25 -1.4308728831541444 1104 1 1.5597234062316225 1104 2 0.750937191841313 1104 3 0.22894166235648683 1104 4 0.2465108345775846 1104 5 0.17662839541230824 1104 6 0.05686939660373014 1104 7 0.08832252838497112 1104 8 0.09142550142770034 1104 9 -0.11853702194874671 1104 10 -0.2135385705728794 1104 11 -0.24182779178178748 1104 12 -0.14877332221884387 1104 13 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21 -0.7004893322153265 1108 22 -0.8516888181855009 1108 23 -0.7261722054425022 1108 24 -0.8772982619939782 1108 25 -0.850574921836845 1109 1 0.7815446548722313 1109 2 0.04256705327608756 1109 3 -0.32934746770839496 1109 4 -0.3718910415644885 1109 5 -0.5092383955413737 1109 6 -0.643382136639346 1109 7 -0.5536827834052787 1109 8 -0.6812883203240077 1109 9 -0.660600038424171 1109 10 -0.8799566632110896 1109 11 -0.8030043755164655 1109 12 -0.7920496166902922 1109 13 -0.6969819868321778 1109 14 -0.8800146277341838 1109 15 -0.8328726361575818 1109 16 -0.9690875909013528 1109 17 -1.0277915987646082 1109 18 -0.9005476144828535 1109 19 -1.2041164893558545 1109 20 -1.058706210551719 1109 21 -1.3039845012974496 1109 22 -1.2991984800212535 1109 23 -1.3536625436067595 1109 24 -1.2556557499166843 1109 25 -1.2770966609672811 1110 1 0.49075097824306724 1110 2 -0.020802517592823477 1110 3 -0.19472717061938027 1110 4 -0.16862175123694811 1110 5 -0.10424222727080235 1110 6 -0.13436728670976805 1110 7 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1.156525574594717 1201 16 1.1964522011425345 1201 17 1.1520873863606411 1201 18 1.343798276181026 1201 19 1.2839811680182365 1201 20 1.1820907253679493 1201 21 1.1931487805943775 1201 22 1.0891917827633648 1201 23 1.2567874677256512 1201 24 1.2638087490761336 1201 25 1.3610276084741741 1202 1 2.67764598437277 1202 2 1.836906694750116 1202 3 1.4106488392345504 1202 4 1.1783295437351526 1202 5 1.1354136585968462 1202 6 1.010786526274373 1202 7 1.0444402453007555 1202 8 0.9938848541080603 1202 9 1.0539725650812386 1202 10 1.1455149501557784 1202 11 1.096473605878515 1202 12 1.227033507037099 1202 13 1.0957117489445594 1202 14 1.2267057379782784 1202 15 1.2498199018709286 1202 16 1.3577554441926765 1202 17 1.3129917285799806 1202 18 1.4363016089770468 1202 19 1.4010298355533166 1202 20 1.303139020414065 1202 21 1.4151470314210428 1202 22 1.5482822561946605 1202 23 1.4912120920781575 1202 24 1.4645612421382523 1202 25 1.4629994583284471 1203 1 2.575813032731496 1203 2 1.8324715989951947 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9 -0.7047169483651459 2314 10 -0.8084936469553554 2314 11 -1.5357448627564452 2314 12 -1.2076066413979731 2314 13 -0.9815144075627771 2314 14 -0.9536247666122719 2314 15 -1.1602209029901758 2314 16 -1.388865403247415 2314 17 -1.5678203768621444 2314 18 -2.3743189279912755 2314 19 -2.2064377497155405 2314 20 -2.5424003503631525 2314 21 -1.7708283304264185 2314 22 -1.3081762958241399 2314 23 -1.2550603986446518 2314 24 -1.3120746483982728 2314 25 -1.0620776051999954 2315 1 -2.750292864222395 2315 2 -1.1571396605499764 2315 3 -0.4457539729307882 2315 4 -0.42522094379634723 2315 5 -0.2461519372593699 2315 6 -0.278343418119249 2315 7 -0.2751768090910929 2315 8 -0.24053322249199027 2315 9 -0.315531973036137 2315 10 -0.37230402938444485 2315 11 -0.5417840349248948 2315 12 -0.41105007112872727 2315 13 -0.40506880099970743 2315 14 -0.4935205746725657 2315 15 -0.9260801502698115 2315 16 -0.8052981218278035 2315 17 -0.7748341900492477 2315 18 -0.42215990366630507 2315 19 -0.5531768603163272 2315 20 -0.4421173824371323 2315 21 -0.45989375231354757 2315 22 -0.3976935405126625 2315 23 -0.4006907169649807 2315 24 -0.3568298071394267 2315 25 -0.2457732595776818 2316 1 1.21053560030008 2316 2 0.39876340788177345 2316 3 -0.1626608610429976 2316 4 -0.31134414178110603 2316 5 -0.48057102191111767 2316 6 -0.6365998179800414 2316 7 -0.5459705357142397 2316 8 -0.5262086056472626 2316 9 -0.7311622557520323 2316 10 -0.698506300212479 2316 11 -0.7964368650253064 2316 12 -0.7850860253152447 2316 13 -0.6529136832728923 2316 14 -0.827107466555546 2316 15 -0.9502095586906876 2316 16 -0.8330623577393155 2316 17 -0.811550469798135 2316 18 -0.8197135787177459 2316 19 -0.9452527067380956 2316 20 -0.7973154862765881 2316 21 -0.8840662552922467 2316 22 -1.0367835649022012 2316 23 -0.9552894662304965 2316 24 -0.9641275302866603 2316 25 -1.0188863664897525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56372.0 3 5.0 4 477.0 5 2191.0 6 2738.0 7 2567.0 8 2323.0 9 2182.0 10 2172.0 11 2239.0 12 2101.0 13 2171.0 14 2229.0 15 2250.0 16 2430.0 17 2596.0 18 2903.0 19 3425.0 20 4260.0 21 5157.0 22 6237.0 23 7486.0 24 9004.0 25 10359.0 26 11479.0 27 12955.0 28 14951.0 29 18219.0 30 23153.0 31 29520.0 32 39103.0 33 53139.0 34 74508.0 35 107581.0 36 185099.0 37 315915.0 38 925402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.787392815424475 19.594840500278885 12.181023755102125 24.436742929194516 2 14.81108408382569 20.33050688955332 41.4245503357572 23.433858690863786 3 18.477130468212913 25.062537298203193 29.967708388088898 26.492623845494993 4 12.397990968944733 17.009208831945386 38.411099036038166 32.181701163071715 5 13.107371353968633 37.845411464306586 35.85591768137805 13.191299500346737 6 31.253093168687062 37.17271635958572 18.392103323807703 13.18208714791951 7 27.501600632623624 31.740106750081708 22.76151140959802 17.996781207696646 8 24.766995960146712 36.878980136743806 20.95059979375567 17.403424109353814 9 26.491769335366104 15.007159339074539 20.541637479912644 37.959433845646714 10 15.306033650442194 28.5608063747892 34.725711563960296 21.407448410808307 11 33.87691431402449 22.176404516949376 25.270361369339973 18.67631979968616 12 23.333690389256187 24.668633220826386 32.013445215433464 19.98423117448396 13 28.98935019500949 22.22181015780007 25.946048405207673 22.84279124198277 14 20.926245334550156 22.438348079253547 27.88366903284652 28.751737553349777 15 22.837194004798388 31.24628186768037 23.585717835384557 22.330806292136685 16 21.985848526206222 27.94028899211755 28.19736343951581 21.876499042160415 17 21.55170545938913 28.737808382282083 28.049568153890124 21.660918004438663 18 21.434536665638177 27.137920394939837 30.26241900647948 21.165123932942507 19 23.402363379497938 26.786870525334304 27.93579984560941 21.87496624955835 20 23.583414687868764 27.141777153587928 28.451049100399818 20.82375905814349 21 22.873535946605703 26.56307659526943 27.571219246322272 22.992168211802596 22 23.25208145250276 28.028220817868725 27.628211558395126 21.091486171233388 23 22.195591101864036 27.72733944292663 28.16694176144776 21.910127693761574 24 23.120838082629767 26.586625878136534 28.762924917038752 21.52961112219495 25 22.640128643365358 27.232744233637472 28.398187935661813 21.728939187335353 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 942.0 1 942.0 2 1399.5 3 1857.0 4 1857.0 5 1857.0 6 2933.5 7 4010.0 8 4010.0 9 4010.0 10 4746.5 11 5483.0 12 5483.0 13 5483.0 14 7647.5 15 9812.0 16 9812.0 17 9812.0 18 16990.5 19 24169.0 20 24169.0 21 24169.0 22 37988.0 23 51807.0 24 51807.0 25 51807.0 26 73783.5 27 95760.0 28 95760.0 29 95760.0 30 123426.5 31 151093.0 32 151093.0 33 151093.0 34 170522.0 35 189951.0 36 189951.0 37 189951.0 38 202834.0 39 215717.0 40 215717.0 41 215717.0 42 226890.0 43 238063.0 44 238063.0 45 238063.0 46 244027.0 47 249991.0 48 249991.0 49 249991.0 50 240959.5 51 231928.0 52 231928.0 53 231928.0 54 206336.0 55 180744.0 56 180744.0 57 180744.0 58 155382.5 59 130021.0 60 130021.0 61 130021.0 62 107031.5 63 84042.0 64 84042.0 65 84042.0 66 64950.0 67 45858.0 68 45858.0 69 45858.0 70 33576.0 71 21294.0 72 21294.0 73 21294.0 74 15072.5 75 8851.0 76 8851.0 77 8851.0 78 6199.0 79 3547.0 80 3547.0 81 3547.0 82 2400.0 83 1253.0 84 1253.0 85 1253.0 86 847.0 87 441.0 88 441.0 89 441.0 90 298.5 91 156.0 92 156.0 93 156.0 94 108.5 95 61.0 96 61.0 97 61.0 98 54.0 99 47.0 100 47.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36057358937140005 2 0.31131574432764325 3 0.3316044291996807 4 0.3215884961615863 5 0.30571709457814433 6 0.2629310831897716 7 0.36160086455479434 8 0.3328885231789236 9 0.2762342968147279 10 0.2502955984340217 11 0.2642665409281842 12 0.2615442616921893 13 0.28013794251162616 14 0.2686838242167797 15 0.36365541492158293 16 0.4191282748248753 17 0.4353592227225052 18 0.11803391857200532 19 0.12733075898172375 20 0.19297364320061966 21 0.2012945721861135 22 0.15116354323647155 23 0.2641638134098448 24 0.3665831491942567 25 0.405465514885731 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1946898.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.91906424746703 #Duplication Level Percentage of deduplicated Percentage of total 1 83.1132322864177 58.94312660335109 2 11.45977328836371 16.254327961977452 3 2.907238193924923 6.185358367729551 4 1.0303045052762034 2.922729256165513 5 0.4840152861128693 1.716295558629736 6 0.2601313982342121 1.1068965206493313 7 0.16184768953644812 0.8034660684777623 8 0.11602589050271894 0.658275806634552 9 0.07777555760599282 0.4964192790067781 >10 0.3487990743782162 4.250438682369501 >50 0.021020322798877274 1.0385103492921808 >100 0.01649517065702725 2.3363255100038263 >500 0.0019612190687461374 0.945848697758229 >1k 0.0013074793791640845 2.0202763590589874 >5k 7.263774328689358E-5 0.32170497889549127 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6245 0.32076667601487086 No Hit GGTATCAACGCAGAGTACTTTTTTT 4140 0.21264596296262056 No Hit TATCAACGCAGAGTACTTTTTTTTT 3848 0.1976477452850637 No Hit GTCCTACAGTGGACATTTCTAAATT 3729 0.19153545794386762 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3579 0.18383089406841036 No Hit CTGTAGGACGTGGAATATGGCAAGA 3405 0.17489359997287993 No Hit CTTTAGGACGTGAAATATGGCGAGG 2965 0.15229354593820527 No Hit GTCCTACAGTGTGCATTTCTCATTT 2340 0.12019119645713336 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.1363759169715105E-5 0.0 4 0.0 0.0 0.0 5.1363759169715105E-5 0.0 5 0.0 0.0 0.0 5.1363759169715105E-5 0.0 6 0.0 0.0 0.0 5.1363759169715105E-5 0.0 7 0.0 0.0 0.0 5.1363759169715105E-5 0.0 8 0.0 0.0 0.0 5.1363759169715105E-5 0.0 9 0.0 0.0 0.0 5.1363759169715105E-5 0.0 10 0.0 0.0 0.0 5.1363759169715105E-5 0.0 11 0.0 0.0 0.0 2.0545503667886042E-4 0.0 12 0.0 0.0 0.0 2.0545503667886042E-4 0.0 13 0.0 0.0 0.0 2.0545503667886042E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1165 0.0 13.700242 1 TAGGACC 765 0.0 13.166082 4 CGCCCTA 100 1.9081854E-6 11.410872 16 GGACCTG 1430 0.0 11.228982 6 GATTTAC 60 0.005874403 11.083876 1 GTCTTAG 210 0.0 10.857674 1 AGGACCT 1470 0.0 10.730079 5 TTAGGAC 1235 0.0 10.694764 3 TGTAGGA 2195 0.0 10.692661 2 GTAGGAC 2250 0.0 10.473517 3 AGGACGT 2055 0.0 10.44982 5 GACGTGA 1120 0.0 10.34979 7 ACCTTTT 505 0.0 10.167841 15 GTATTAA 235 0.0 10.106877 1 GTCCTAC 2050 0.0 10.102933 1 GGACGTG 2080 0.0 10.095287 6 ACGTGAA 1260 0.0 10.083616 8 TGGCGAG 2265 0.0 10.067758 18 CTGTAGG 2125 0.0 10.059316 1 GGCGAGG 1145 0.0 10.04576 19 >>END_MODULE