FastQCFastQC Report
Thu 26 May 2016
SRR1512302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512302_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946898
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68350.35107129392500275No Hit
GGTATCAACGCAGAGTACTTTTTTT46910.24094739426513356No Hit
TATCAACGCAGAGTACTTTTTTTTT45190.23211282768794256No Hit
GTCCTACAGTGGACATTTCTAAATT37330.1917409129805465No Hit
GTCCTAAAGTGTGTATTTCTCATTT36730.18865908743036358No Hit
CTGTAGGACGTGGAATATGGCAAGA35500.18234134505248864No Hit
CTTTAGGACGTGAAATATGGCGAGG32030.1645181206205975No Hit
GTCCTACAGTGTGCATTTCTCATTT24110.12383802335818313No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20600.10580934388961312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11750.014.5679351
TAGGACC7450.012.3740174
TATGCCG657.996731E-411.6972295
TCTAGTA1406.7666406E-1011.5393832
GGCGAGG9300.011.53363119
CTAGTAC1251.8239007E-811.40362553
CCCGTAC752.090046E-411.39073816
GGATAGA1900.011.0111441
CGAATGG951.3728846E-510.99106119
GTACTAG1303.2187017E-810.9726441
TATGTCG1605.529728E-1010.67881616
TAAGACT2150.010.6088434
GTATCAA26350.010.5742691
TGGCGAG20100.010.53096718
GTCCTAC19900.010.4653651
GACGTGA12450.010.3089897
GTCCTAA11950.010.1860411
CCAATAC1409.6450094E-810.1818083
ATGCCGT750.002632105510.1407296
TGTAGGA22050.010.1279372
Produced by FastQC (version 0.11.5)