FastQCFastQC Report
Thu 26 May 2016
SRR1512301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881876
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58700.31192278343525287No Hit
GTCCTACAGTGGACATTTCTAAATT52840.28078364355568597No Hit
GTCCTAAAGTGTGTATTTCTCATTT48090.25554287317549085No Hit
CTGTAGGACGTGGAATATGGCAAGA46620.24773151897361992No Hit
CTTTAGGACGTGAAATATGGCGAGG41560.22084345621071738No Hit
GGTATCAACGCAGAGTACTTTTTTT36690.19496502426302265No Hit
TATCAACGCAGAGTACTTTTTTTTT34210.1817866852013629No Hit
GTCCTACAGTGTGCATTTCTCATTT32060.17036191545032722No Hit
CTGTAGGACCTGGAATATGGCGAGA23640.12561932879743404No Hit
CTGAAGGACCTGGAATATGGCGAGA21160.11244098973577431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10250.014.1823834
GGTATCA10150.013.6653891
CCGTGTA550.003100291112.0744649
GTATTAG1406.8030204E-1011.5360221
AGGACCT17900.011.4121385
CCAACGT752.0644927E-411.40578719
TCCAACG2000.011.40152218
TTAAGCG1751.8189894E-1211.39452412
GGCGAGG14150.011.3520519
TAAGCGA1701.0913936E-1111.17796313
GGACCTG18550.011.1140986
CCAACGA1303.2425305E-810.96710319
AAGCGAC1657.8216544E-1110.94904314
AGCGACT1657.8216544E-1110.94904315
ACCGTGC700.0015140610.8377518
TAGGACT3800.010.7514354
TGTAGGA31400.010.6813632
TTAGGAC18800.010.6636573
GTGTAGG3300.010.6517991
AATGTCC7750.010.6454698
Produced by FastQC (version 0.11.5)