FastQCFastQC Report
Thu 26 May 2016
SRR1512301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881876
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71570.380311986549592No Hit
GTCCTACAGTGGACATTTCTAAATT52520.27908321270902015No Hit
CTGTAGGACGTGGAATATGGCAAGA51030.27116558157923265No Hit
GTCCTAAAGTGTGTATTTCTCATTT49670.2639387504809031No Hit
GGTATCAACGCAGAGTACTTTTTTT46060.24475576499195484No Hit
TATCAACGCAGAGTACTTTTTTTTT44490.2364130261505009No Hit
CTTTAGGACGTGAAATATGGCGAGG44250.23513770301550158No Hit
GTCCTACAGTGTGCATTTCTCATTT31990.1699899462026191No Hit
CTGTAGGACCTGGAATATGGCGAGA25830.13725665240430293No Hit
CTGAAGGACCTGGAATATGGCGAGA24360.12944529820243203No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22020.11701089763618856No Hit
ATTTAGAAATGTCCACTGTAGGACG20510.10898698957848446No Hit
GTCCTTCAGTGTGCATTTCTCATTT19360.10287606622327933No Hit
TTTCTAAATTTTCCACCTTTTTCAG19140.10170702001619661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG350.00217861816.27653319
AATCGCC456.792237E-414.76944718
GGTATCA12250.014.2102731
TAGGACC11250.013.9358494
TGGACCG550.003063490412.0937365
TGTAGGA31100.011.151532
GTCCTAC26350.011.0826881
GTAGGAC30850.010.9031033
CGGACAT700.001492386610.85650410
CTGTAGG30550.010.8045241
GCCGGTT1158.8250454E-710.73849911
GCGCCAC1158.834122E-710.73764213
AGGACCT20500.010.6610285
GGACCTG19850.010.5815516
TCCTACA29500.010.4679922
CCAACGT1002.4140818E-510.44410919
CACCTTT6950.010.3829114
CGTGCAT1106.050481E-610.36302810
TGCCGGT1303.8448343E-710.23016810
TCCAACG2151.8189894E-1210.15706218
Produced by FastQC (version 0.11.5)