FastQCFastQC Report
Thu 26 May 2016
SRR1512300_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512300_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836628
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78760.4288293546651799No Hit
GGTATCAACGCAGAGTACTTTTTTT48810.2657587709650512No Hit
TATCAACGCAGAGTACTTTTTTTTT45670.24866222228997922No Hit
GTCCTACAGTGGACATTTCTAAATT35200.191655577503991No Hit
GTCCTAAAGTGTGTATTTCTCATTT32710.17809812329987348No Hit
CTGTAGGACGTGGAATATGGCAAGA31930.17385120993472822No Hit
CTTTAGGACGTGAAATATGGCGAGG29250.15925925119294707No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23430.127570743776094No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21650.11787906968640355No Hit
GTACTGGTTCACTATCGGTCAGTCA20440.11129090920970389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.013.8124181
TAGGACC6400.013.2124214
GGCGAGG9450.012.37086819
GGACCTG10750.011.3128996
AGCGCAT600.005904981411.07609310
AGGACCT11950.011.0514515
GTCGAAA1053.4326404E-610.86609315
GTCCTAC15700.010.5295571
GTATTAC1909.094947E-1210.5008931
TCCAACG1451.4415491E-810.48364918
CCAACGA1002.3866462E-510.45489219
ATTAGAC2001.8189894E-1210.4511743
TAGACAG2750.010.36485
TGGCGAG19600.010.32485718
GTATCAA28450.010.28558351
AAATGTC5200.010.049487
GTCCTAA9950.010.0259771
TAGAAAT5550.09.9290434
TCCTACA17900.09.8726182
GTAGGAC19300.09.8456663
Produced by FastQC (version 0.11.5)