##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512300_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1836628 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.603875689578945 31.0 30.0 34.0 16.0 34.0 2 29.500016878758245 31.0 30.0 34.0 16.0 34.0 3 30.16851425547253 31.0 30.0 34.0 25.0 34.0 4 33.81663897098378 37.0 35.0 37.0 30.0 37.0 5 33.98530676870874 37.0 35.0 37.0 30.0 37.0 6 34.13921545353768 37.0 35.0 37.0 30.0 37.0 7 34.0796508601633 37.0 35.0 37.0 30.0 37.0 8 34.076493987895205 37.0 35.0 37.0 30.0 37.0 9 35.60490474935588 39.0 37.0 39.0 30.0 39.0 10 35.52833344585839 39.0 37.0 39.0 30.0 39.0 11 35.55519245051257 39.0 37.0 39.0 30.0 39.0 12 35.48731044065538 39.0 37.0 39.0 30.0 39.0 13 35.45850874537467 39.0 37.0 39.0 30.0 39.0 14 36.63284290558567 40.0 37.0 41.0 30.0 41.0 15 36.55166424556307 40.0 37.0 41.0 30.0 41.0 16 36.47242337588232 40.0 37.0 41.0 29.0 41.0 17 36.434318762427665 40.0 37.0 41.0 29.0 41.0 18 36.21166126183419 40.0 37.0 41.0 27.0 41.0 19 36.312869018658105 40.0 37.0 41.0 28.0 41.0 20 36.24166570475894 40.0 37.0 41.0 28.0 41.0 21 36.188208499489285 40.0 37.0 41.0 28.0 41.0 22 36.09797792476212 40.0 37.0 41.0 27.0 41.0 23 35.965651726969206 40.0 37.0 41.0 27.0 41.0 24 35.819765352591816 40.0 37.0 41.0 27.0 41.0 25 35.69024320657204 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0755690315849762 1101 2 0.1483158124092938 1101 3 -0.2927478866566027 1101 4 -0.39727764276682365 1101 5 -0.538157667267015 1101 6 -0.6487140177165074 1101 7 -0.6455240854099387 1101 8 -0.8823987617774804 1101 9 -0.7572615873127546 1101 10 -1.1977637286310596 1101 11 -0.8237956305286716 1101 12 -1.0570587069355994 1101 13 -1.0705385343112965 1101 14 -1.3840035287862023 1101 15 -1.4480037927875102 1101 16 -1.528832116101853 1101 17 -1.5344615637937622 1101 18 -1.5051826548402474 1101 19 -1.587305358669596 1101 20 -1.6534858334763598 1101 21 -1.7477104885819017 1101 22 -1.9591418571376025 1101 23 -2.0849023856958624 1101 24 -2.1918267062720886 1101 25 -2.2974986722227584 1102 1 0.8149168482574503 1102 2 -0.08204623793854182 1102 3 -0.5993505116050422 1102 4 -0.6539967340947825 1102 5 -0.6327591790226279 1102 6 -0.7539891886178722 1102 7 -0.8439627756761041 1102 8 -0.8947056480252158 1102 9 -1.0882905594339505 1102 10 -1.2115749870714083 1102 11 -1.3220122501851819 1102 12 -1.6275522815837675 1102 13 -1.2949583499955324 1102 14 -1.615022277707972 1102 15 -1.7956919653267178 1102 16 -1.7766818719874635 1102 17 -1.9097612490754088 1102 18 -1.688411141217479 1102 19 -1.8431980747110046 1102 20 -2.067773535606406 1102 21 -1.971199567152155 1102 22 -1.9592682272415587 1102 23 -2.0678012448861978 1102 24 -2.04454199513782 1102 25 -2.2615808099505585 1103 1 1.0016978340032807 1103 2 0.1326614178370633 1103 3 -0.3151815699594174 1103 4 -0.23767691575590533 1103 5 -0.604224886626497 1103 6 -0.6126793863979572 1103 7 -0.6665303894705303 1103 8 -0.627663937045817 1103 9 -0.9534230184477224 1103 10 -0.9550279059880964 1103 11 -0.8336269550457658 1103 12 -1.004183644481401 1103 13 -0.9965405640303473 1103 14 -1.0615934374081704 1103 15 -1.2441851550313459 1103 16 -1.2419495652453207 1103 17 -1.347952144988966 1103 18 -1.270633162303909 1103 19 -1.4290432022185229 1103 20 -1.477888018847878 1103 21 -1.3206556353229928 1103 22 -1.7859913657408555 1103 23 -1.8552119483571659 1103 24 -1.6222457815635494 1103 25 -1.914897309992483 1104 1 1.056785207171263 1104 2 0.3688687561582711 1104 3 -0.1490405942496693 1104 4 -0.1124456715209945 1104 5 -0.38523851786560215 1104 6 -0.2710125062093738 1104 7 -0.3645132479311215 1104 8 -0.5156693434207327 1104 9 -0.5277164744280469 1104 10 -0.5834389185944318 1104 11 -0.659113785013993 1104 12 -0.44526184474787556 1104 13 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1107 11 -0.8182595629971274 1107 12 -0.7544798186418333 1107 13 -0.8839607187714975 1107 14 -0.993468366677341 1107 15 -0.975208879047166 1107 16 -1.124472639611895 1107 17 -1.2191475425786038 1107 18 -1.3959760725319796 1107 19 -1.2371409690581956 1107 20 -1.3347273857299484 1107 21 -1.2797112587642303 1107 22 -1.399604776449543 1107 23 -1.33952940121641 1107 24 -1.220037690386313 1107 25 -1.1415203229038156 1108 1 -0.5721357043568851 1108 2 -0.8457364107927425 1108 3 -1.213386804339848 1108 4 -0.981454628381826 1108 5 -0.9810099046557568 1108 6 -1.0165234128630445 1108 7 -1.0555792362323686 1108 8 -1.310354634671036 1108 9 -1.4904647683593168 1108 10 -1.4603780484993791 1108 11 -1.5073163197446746 1108 12 -1.6631999388253789 1108 13 -1.725399174099529 1108 14 -1.9740380121563206 1108 15 -1.9702509671340707 1108 16 -1.8358495383449949 1108 17 -1.8467516045644246 1108 18 -1.6598412952701054 1108 19 -1.8784826779946329 1108 20 -1.8564656867406768 1108 21 -1.6716278252211652 1108 22 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-0.308284937727592 2315 25 0.04241957593875867 2316 1 1.529406972772481 2316 2 0.841862391138207 2316 3 0.21606076177634392 2316 4 0.059953837265503296 2316 5 -0.0016284445295013938 2316 6 -0.2905166227169147 2316 7 -0.1092854563163499 2316 8 -0.16789969488053202 2316 9 -0.2056507832698884 2316 10 -0.2958930835314746 2316 11 -0.280253261975389 2316 12 -0.2545049608403076 2316 13 -0.3270112217121621 2316 14 -0.370705780589482 2316 15 -0.4020634546371866 2316 16 -0.3160059075755086 2316 17 -0.36644649685654684 2316 18 -0.25721337813926937 2316 19 -0.3354933814521388 2316 20 -0.3043947737843098 2316 21 -0.34605378447212587 2316 22 -0.3589010076339889 2316 23 -0.35348138735339063 2316 24 -0.31640580311619715 2316 25 -0.28523264586889496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63215.0 3 5.0 4 486.0 5 2569.0 6 3009.0 7 2812.0 8 2554.0 9 2443.0 10 2350.0 11 2394.0 12 2367.0 13 2368.0 14 2365.0 15 2442.0 16 2574.0 17 2643.0 18 3126.0 19 3687.0 20 4453.0 21 5545.0 22 6607.0 23 7974.0 24 9178.0 25 11001.0 26 12021.0 27 13508.0 28 15581.0 29 18957.0 30 23537.0 31 30794.0 32 39716.0 33 53397.0 34 73706.0 35 105412.0 36 178827.0 37 299773.0 38 823232.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34346094665245 19.446787956146945 12.085435208336976 24.12431588886363 2 14.929261319417705 20.18344815910127 41.419306567050874 23.467983954430142 3 18.774382936232648 25.07764848805311 29.615346378352157 26.532622197362084 4 12.307504925197623 16.81580170528994 38.4505139847211 32.42617938479134 5 13.068961864059686 37.906307715327664 35.677260529514754 13.347469891097901 6 31.382026924785933 37.011700305363014 18.387012955820076 13.219259814030979 7 27.58925570834893 31.77199937704748 22.52377738736991 18.11496752723368 8 25.019801536708496 36.521681180510264 20.88944679149555 17.569070491285686 9 26.70453738937691 14.900336851070334 20.4091894282267 37.98593633132606 10 15.408118653975572 28.572808682253807 34.42871248057611 21.590360183194505 11 34.10545794974112 22.25539940444835 24.967533974010802 18.671608671799728 12 23.58878286325969 24.586082457625686 31.73634390077879 20.088790778335834 13 29.192129140535382 22.072900611863723 25.88357718889921 22.85139305870168 14 21.010409040858228 22.4401713531098 27.93359220820484 28.615827397827132 15 23.079457659793157 30.982875509065504 23.724733876154396 22.212932954986943 16 22.028709138463423 27.879934656531287 27.965933047000064 22.12542315800523 17 21.615886116544193 28.424276458497488 28.02697044045413 21.93286698450419 18 21.49357353664267 26.82308446255709 30.301573336037148 21.381768664763097 19 23.314778569520392 26.604805338538117 28.19431798976126 21.886098102180227 20 23.682022566309403 26.653166015456648 28.73647434421642 20.928337074017538 21 23.043782351760196 26.28926947215894 27.771176491126997 22.895771684953868 22 23.36757073323947 27.811252973233536 27.73714706995401 21.084029223572983 23 22.245186620308424 27.433084346123337 28.441971131458804 21.879757902109432 24 23.166639435947065 26.344109230339647 28.943012752173313 21.546238581539974 25 22.617064694920323 27.092306439207377 28.621025753440232 21.66960311243207 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 886.0 1 886.0 2 1373.5 3 1861.0 4 1861.0 5 1861.0 6 3086.5 7 4312.0 8 4312.0 9 4312.0 10 5007.5 11 5703.0 12 5703.0 13 5703.0 14 7530.5 15 9358.0 16 9358.0 17 9358.0 18 16156.0 19 22954.0 20 22954.0 21 22954.0 22 35331.5 23 47709.0 24 47709.0 25 47709.0 26 69267.5 27 90826.0 28 90826.0 29 90826.0 30 115560.5 31 140295.0 32 140295.0 33 140295.0 34 156975.5 35 173656.0 36 173656.0 37 173656.0 38 185435.0 39 197214.0 40 197214.0 41 197214.0 42 207895.5 43 218577.0 44 218577.0 45 218577.0 46 228762.0 47 238947.0 48 238947.0 49 238947.0 50 230935.5 51 222924.0 52 222924.0 53 222924.0 54 198301.5 55 173679.0 56 173679.0 57 173679.0 58 149416.5 59 125154.0 60 125154.0 61 125154.0 62 104378.0 63 83602.0 64 83602.0 65 83602.0 66 64048.0 67 44494.0 68 44494.0 69 44494.0 70 32732.5 71 20971.0 72 20971.0 73 20971.0 74 14744.5 75 8518.0 76 8518.0 77 8518.0 78 5893.5 79 3269.0 80 3269.0 81 3269.0 82 2190.0 83 1111.0 84 1111.0 85 1111.0 86 733.5 87 356.0 88 356.0 89 356.0 90 248.0 91 140.0 92 140.0 93 140.0 94 104.0 95 68.0 96 68.0 97 68.0 98 56.0 99 44.0 100 44.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3547806088113652 2 0.304525467323813 3 0.32352768225247575 4 0.3123659227671581 5 0.2963038786297498 6 0.25644823012607887 7 0.36115097885908304 8 0.32461663439738475 9 0.26995123672295096 10 0.2437619376378886 11 0.2566115729478152 12 0.2548692495159608 13 0.27283695990695994 14 0.2643431331766694 15 0.3571218559229196 16 0.40966379691478083 17 0.4252902601942255 18 0.11880467900957624 19 0.13165431431950292 20 0.19764481430099073 21 0.19884266166039066 22 0.1506020816409202 23 0.2617296480288877 24 0.35864638892579226 25 0.39850203742946316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1836628.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.03671398574346 #Duplication Level Percentage of deduplicated Percentage of total 1 83.37751668139322 57.56109771974898 2 11.101827124932777 15.328673278863056 3 2.946099039055565 6.10166990198858 4 1.058101794281222 2.9219148375837873 5 0.4806473312646701 1.6591156168264958 6 0.2586914869102854 1.0715526115423244 7 0.16521342967619385 0.7984054603811395 8 0.10432256566327179 0.5761669702363389 9 0.07309611087705249 0.45416837700803386 >10 0.37751977931651 4.537353160617966 >50 0.029412084820348166 1.4308544753001398 >100 0.02256561684463264 3.1376690080981846 >500 0.0032454786275463426 1.5510768129356391 >1k 0.0016623183214261748 2.4398733913658694 >5k 7.915801530600834E-5 0.4304083775034736 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7876 0.4288293546651799 No Hit GGTATCAACGCAGAGTACTTTTTTT 4881 0.2657587709650512 No Hit TATCAACGCAGAGTACTTTTTTTTT 4567 0.24866222228997922 No Hit GTCCTACAGTGGACATTTCTAAATT 3520 0.191655577503991 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3271 0.17809812329987348 No Hit CTGTAGGACGTGGAATATGGCAAGA 3193 0.17385120993472822 No Hit CTTTAGGACGTGAAATATGGCGAGG 2925 0.15925925119294707 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2343 0.127570743776094 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2165 0.11787906968640355 No Hit GTACTGGTTCACTATCGGTCAGTCA 2044 0.11129090920970389 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.444760724545199E-5 0.0 0.0 0.0 6 0.0 5.444760724545199E-5 0.0 0.0 0.0 7 0.0 5.444760724545199E-5 0.0 0.0 0.0 8 0.0 5.444760724545199E-5 0.0 0.0 0.0 9 0.0 5.444760724545199E-5 0.0 0.0 0.0 10 0.0 5.444760724545199E-5 0.0 5.444760724545199E-5 0.0 11 0.0 5.444760724545199E-5 0.0 5.444760724545199E-5 0.0 12 0.0 5.444760724545199E-5 0.0 5.444760724545199E-5 0.0 13 0.0 5.444760724545199E-5 0.0 5.444760724545199E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1245 0.0 13.812418 1 TAGGACC 640 0.0 13.212421 4 GGCGAGG 945 0.0 12.370868 19 GGACCTG 1075 0.0 11.312899 6 AGCGCAT 60 0.0059049814 11.076093 10 AGGACCT 1195 0.0 11.051451 5 GTCGAAA 105 3.4326404E-6 10.866093 15 GTCCTAC 1570 0.0 10.529557 1 GTATTAC 190 9.094947E-12 10.500893 1 TCCAACG 145 1.4415491E-8 10.483649 18 CCAACGA 100 2.3866462E-5 10.454892 19 ATTAGAC 200 1.8189894E-12 10.451174 3 TAGACAG 275 0.0 10.3648 5 TGGCGAG 1960 0.0 10.324857 18 GTATCAA 2845 0.0 10.2855835 1 AAATGTC 520 0.0 10.04948 7 GTCCTAA 995 0.0 10.025977 1 TAGAAAT 555 0.0 9.929043 4 TCCTACA 1790 0.0 9.872618 2 GTAGGAC 1930 0.0 9.845666 3 >>END_MODULE