FastQCFastQC Report
Thu 26 May 2016
SRR1512300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512300_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836628
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92430.5032592337697128No Hit
GGTATCAACGCAGAGTACTTTTTTT61430.3344716513088116No Hit
TATCAACGCAGAGTACTTTTTTTTT58110.3163950457033215No Hit
GTCCTACAGTGGACATTTCTAAATT36010.19606583369087263No Hit
CTGTAGGACGTGGAATATGGCAAGA35820.19503132915320903No Hit
GTCCTAAAGTGTGTATTTCTCATTT35240.19187336793297283No Hit
CTTTAGGACGTGAAATATGGCGAGG31880.17357897189850094No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27690.15076542446265656No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24060.1310009430325575No Hit
GTACTGGTTCACTATCGGTCAGTCA21460.11684456514873998No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20160.10976637620683122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14500.015.0214371
CCCTTAT957.274684E-813.0155871
GCACCGT550.003055088512.0981456
TAGGACC6200.011.650864
GTCCTAA10100.010.9239731
ATAAGAC1605.4023985E-1010.6924383
GTATCAA32150.010.5024681
AGGACGT19750.010.3949585
TGTAGGA20350.010.1340722
GGCGAGG8250.010.12531819
GTAGGAC20100.010.1191013
ATAGGAC2350.010.11105253
GTGCGCC856.597543E-410.05826211
CACCTTT5100.010.05223514
CGAATGG856.6423015E-410.05086719
GGACGTG19900.010.0311256
CCTACAC2950.09.9876643
TAGGACG20600.09.91994
TGTCCAC6650.09.85659110
GACGTGG10750.09.8149057
Produced by FastQC (version 0.11.5)