FastQCFastQC Report
Thu 26 May 2016
SRR1512299_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512299_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638343
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50110.3058578087738648No Hit
GGTATCAACGCAGAGTACTTTTTTT31880.19458684780903632No Hit
TATCAACGCAGAGTACTTTTTTTTT29170.17804574499967346No Hit
GTCCTACAGTGGACATTTCTAAATT20250.12360049147217647No Hit
GTCCTAAAGTGTGTATTTCTCATTT19370.11822921085511398No Hit
CTGTAGGACGTGGAATATGGCAAGA18670.11395660127335973No Hit
CTTTAGGACGTGAAATATGGCGAGG17310.10565553122880862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCC350.002167851416.2884227
GGTATCA8500.013.3009881
GTGCGCC1052.7412534E-711.75484511
GCGTGCG907.5780263E-611.5948999
GGCGAGG5650.011.43833819
ATTAGGC752.0707327E-411.4018963
GACGTGA7050.010.7819357
TGCGCCA1158.8525485E-710.73564612
GGACGTG12250.010.7034926
GTATCAA19250.010.6605311
TAGGACC4100.010.6603094
AGGACGT12750.010.4331075
GTATAAA2650.010.3969991
GTCCTAA5700.010.3341021
TTAGGAC7550.010.3195973
TGGCGAG10900.010.28422118
GTGTAGG2500.010.2607611
ACGTGAA8000.010.1957448
TAGGACG13350.010.1065494
GCAATAC856.5832364E-410.0604963
Produced by FastQC (version 0.11.5)