##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512299_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1638343 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.77142149110412 31.0 30.0 34.0 16.0 34.0 2 29.67550384748493 31.0 30.0 34.0 19.0 34.0 3 30.333253781412072 31.0 31.0 34.0 26.0 34.0 4 33.99217013775504 37.0 35.0 37.0 30.0 37.0 5 34.15685604296536 37.0 35.0 37.0 30.0 37.0 6 34.30700408888737 37.0 35.0 37.0 31.0 37.0 7 34.237388629853456 37.0 35.0 37.0 31.0 37.0 8 34.22964238868173 37.0 35.0 37.0 30.0 37.0 9 35.774947004381865 39.0 37.0 39.0 30.0 39.0 10 35.69478552415459 39.0 37.0 39.0 30.0 39.0 11 35.73026710523987 39.0 37.0 39.0 30.0 39.0 12 35.66419791215881 39.0 37.0 39.0 30.0 39.0 13 35.63555616864112 39.0 37.0 39.0 30.0 39.0 14 36.837056098753436 40.0 38.0 41.0 30.0 41.0 15 36.765257946596044 40.0 38.0 41.0 30.0 41.0 16 36.68515017917493 40.0 37.0 41.0 30.0 41.0 17 36.63075009323445 40.0 37.0 41.0 30.0 41.0 18 36.40471989076769 40.0 37.0 41.0 29.0 41.0 19 36.50557483994499 40.0 37.0 41.0 30.0 41.0 20 36.429822692806084 40.0 37.0 41.0 29.0 41.0 21 36.375133900532425 40.0 37.0 41.0 29.0 41.0 22 36.28996064926575 40.0 37.0 41.0 29.0 41.0 23 36.16549098693009 40.0 37.0 41.0 29.0 41.0 24 36.023076364351056 40.0 37.0 41.0 27.0 41.0 25 35.903585512923726 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.293544296521432 1101 2 0.3046556624892034 1101 3 0.013334245993846139 1101 4 -0.2518580615968986 1101 5 -0.31489527539763174 1101 6 -0.25064494478117894 1101 7 -0.3383516217384184 1101 8 -0.6897862329121409 1101 9 -0.714177481439286 1101 10 -0.6459534432392076 1101 11 -0.43480760174340816 1101 12 -0.8279456642713541 1101 13 -0.898306016320447 1101 14 -1.1250309591270735 1101 15 -1.265954623419674 1101 16 -1.2983698949150408 1101 17 -1.305474479617665 1101 18 -1.2458425327888065 1101 19 -1.2823462486062809 1101 20 -1.2535076950764008 1101 21 -1.2239700465614405 1101 22 -1.5714634515808967 1101 23 -1.5326743935873424 1101 24 -1.65853521386056 1101 25 -1.8394954117768307 1102 1 1.0101664117545788 1102 2 0.19551080387231323 1102 3 -0.16699321934956757 1102 4 -0.35129267683004173 1102 5 -0.6690557885513755 1102 6 -0.5577495446551524 1102 7 -0.6468641453679069 1102 8 -0.7069766865983382 1102 9 -0.7875913519496791 1102 10 -0.7249780868277398 1102 11 -0.7957985142355355 1102 12 -0.9016401349707834 1102 13 -0.7381756110746949 1102 14 -0.9817464108441527 1102 15 -1.0900564566742474 1102 16 -1.0717397062729432 1102 17 -1.3005492078785394 1102 18 -1.3181672315128097 1102 19 -1.190434918266014 1102 20 -1.3663065096636728 1102 21 -1.4617048646143687 1102 22 -1.4313713457995476 1102 23 -1.4289507798507302 1102 24 -1.752680269389863 1102 25 -1.9099235978196774 1103 1 1.2086270857005381 1103 2 0.3585366481857193 1103 3 -0.20327800524993478 1103 4 -0.2997533507760082 1103 5 -0.345377629253349 1103 6 -0.35512255672147575 1103 7 -0.4251830894001074 1103 8 -0.49808847171811266 1103 9 -0.6502393595487348 1103 10 -0.7327946279406987 1103 11 -0.8015189667931608 1103 12 -0.8284898185002092 1103 13 -0.8508577109721855 1103 14 -0.9150360119877732 1103 15 -0.9115644648375891 1103 16 -1.121976471407578 1103 17 -1.005043043050506 1103 18 -0.8375422373907924 1103 19 -0.9140510060689664 1103 20 -0.9772522535341182 1103 21 -1.1526664085017373 1103 22 -1.3316760604117377 1103 23 -1.0801712841346003 1103 24 -1.1622957082635423 1103 25 -1.1149734901350428 1104 1 1.4711339955955083 1104 2 0.6308371825509305 1104 3 0.1148581657469343 1104 4 0.00564097389692364 1104 5 -0.05801352385442016 1104 6 -0.0898424756453764 1104 7 -0.1853037822322463 1104 8 -0.11378314649238774 1104 9 -0.19795448761212242 1104 10 -0.3977522472515531 1104 11 -0.0935219150150175 1104 12 -0.25676510871579694 1104 13 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1105 24 -0.7032936474379667 1105 25 -0.6224428676943461 1106 1 0.989880434341945 1106 2 0.23136519774561393 1106 3 -0.347715433493331 1106 4 -0.4573656737763869 1106 5 -0.45604391321813864 1106 6 -0.503644944781179 1106 7 -0.5487073909691844 1106 8 -0.6638439252198367 1106 9 -0.9925508788751785 1106 10 -0.857304004136644 1106 11 -0.725865694692132 1106 12 -0.7788334847841725 1106 13 -0.7317383079871078 1106 14 -1.0867933789988697 1106 15 -1.073394927906854 1106 16 -1.1011218981201694 1106 17 -1.0506852167971559 1106 18 -0.8987940552246982 1106 19 -0.9009063447601235 1106 20 -0.9990165091789649 1106 21 -1.168240078612719 1106 22 -0.9394510317090976 1106 23 -1.2517545217924706 1106 24 -1.1938272811682538 1106 25 -1.3087622386999058 1107 1 0.6580400417248562 1107 2 0.02768483926913845 1107 3 -0.24333689940606007 1107 4 -0.07984129524230354 1107 5 -0.036197972750841245 1107 6 0.030735082958074145 1107 7 0.17592975828932111 1107 8 0.10620024420297369 1107 9 -0.0685500253597624 1107 10 0.07802385247276789 1107 11 -0.0819493392029571 1107 12 -0.04036042386450589 1107 13 -0.0090309570855851 1107 14 -0.09323827390978323 1107 15 -0.007967850130263798 1107 16 -0.2207984157772671 1107 17 -0.39975723216043235 1107 18 -0.16304285496864424 1107 19 -0.2027746900071108 1107 20 -0.26957876245966617 1107 21 -0.43850317473989264 1107 22 -0.24416286356656514 1107 23 -0.2649400547061589 1107 24 -0.21530044877734156 1107 25 -0.28240781642315227 1108 1 -0.28924925152904635 1108 2 -0.4652066711633225 1108 3 -0.7533235201621125 1108 4 -0.4551376696061311 1108 5 -0.5967940152080757 1108 6 -0.5978016919821201 1108 7 -0.6049710236240955 1108 8 -0.6418155493529198 1108 9 -0.6962306758018855 1108 10 -0.7721085914173642 1108 11 -0.6496816595169292 1108 12 -0.9022342372432632 1108 13 -0.8629675360366136 1108 14 -0.9613256304833939 1108 15 -0.9290254070329169 1108 16 -0.9588480211426926 1108 17 -0.8805326398514097 1108 18 -0.8843218191519924 1108 19 -0.7985167885296747 1108 20 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1.1089191575084953 1201 14 1.2845167262033286 1201 15 1.3313185167413408 1201 16 1.4402258482363592 1201 17 1.3870655794580316 1201 18 1.456371662370124 1201 19 1.3774081036521864 1201 20 1.4598330227195007 1201 21 1.4460604715272254 1201 22 1.4126918116457858 1201 23 1.3905468004737216 1201 24 1.3247639745020479 1201 25 1.2898421431165161 1202 1 2.5867751438119484 1202 2 1.691686128198075 1202 3 1.441990963367335 1202 4 1.2782038974022782 1202 5 1.1004873146197838 1202 6 0.9949758296502935 1202 7 1.0803734089929904 1202 8 1.02070470132265 1202 9 1.212920257241315 1202 10 1.2823459414924088 1202 11 1.0982530703780782 1202 12 1.160994230217483 1202 13 1.1853262279686376 1202 14 1.2778846031296354 1202 15 1.426570842033101 1202 16 1.4555167478067403 1202 17 1.2908388718984156 1202 18 1.4772142658948653 1202 19 1.5038904024385857 1202 20 1.377099986494997 1202 21 1.4398771735266607 1202 22 1.3329158540173438 1202 23 1.38009002456468 1202 24 1.5253814560072954 1202 25 1.548418982046158 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-0.6971906190822068 2314 10 -0.7156616254071935 2314 11 -1.5530075660134202 2314 12 -0.8661904971649292 2314 13 -0.7778385718393537 2314 14 -0.7845794557189834 2314 15 -0.8908189181646193 2314 16 -1.1476150988508351 2314 17 -1.4284907779905751 2314 18 -1.9366052993895053 2314 19 -2.109570440558791 2314 20 -2.510833030388625 2314 21 -1.7455942495077892 2314 22 -1.3557968811103578 2314 23 -0.9686435008872536 2314 24 -1.1892873988869468 2314 25 -1.143290349111922 2315 1 -2.6516018789651845 2315 2 -1.3632513098344 2315 3 -0.8166345800402155 2315 4 -0.709293242557969 2315 5 -0.5422513809329104 2315 6 -0.5143310761680411 2315 7 -0.5848160377968128 2315 8 -0.6681165248829473 2315 9 -0.6474385824125193 2315 10 -0.6120184903803221 2315 11 -0.8650130837993686 2315 12 -0.8831844112299905 2315 13 -0.7512894028776245 2315 14 -0.9016381291392435 2315 15 -1.285091446716514 2315 16 -1.1916418307422916 2315 17 -1.2030470921480827 2315 18 -0.9954956273751776 2315 19 -0.9929130825478865 2315 20 -1.1213129718403962 2315 21 -1.2303664121332147 2315 22 -1.0254675954373411 2315 23 -1.025869344925539 2315 24 -0.8999100998094676 2315 25 -0.8431165340396021 2316 1 0.991314326970862 2316 2 0.2886260390810875 2316 3 -0.1878876646384633 2316 4 -0.3967083249058021 2316 5 -0.6252645672864219 2316 6 -0.5813313063982406 2316 7 -0.6766387718939058 2316 8 -0.5500844337117883 2316 9 -0.7178367347341137 2316 10 -0.6769954916806356 2316 11 -0.8510077512140128 2316 12 -0.8484897742965245 2316 13 -0.7685965931936849 2316 14 -0.9368035625070519 2316 15 -0.8259190026770469 2316 16 -0.8786487408909025 2316 17 -0.8025805111962256 2316 18 -0.6093166947353481 2316 19 -0.6947098508941565 2316 20 -0.6997266209241744 2316 21 -0.7314036640277521 2316 22 -0.8714328517548893 2316 23 -0.8974821642078084 2316 24 -0.9713623254220849 2316 25 -1.0047223248154822 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52079.0 3 4.0 4 402.0 5 2113.0 6 2582.0 7 2374.0 8 2070.0 9 2133.0 10 1964.0 11 2042.0 12 1898.0 13 2073.0 14 2003.0 15 2101.0 16 2192.0 17 2355.0 18 2628.0 19 3154.0 20 3875.0 21 4592.0 22 5643.0 23 6751.0 24 7919.0 25 9292.0 26 10191.0 27 11485.0 28 13485.0 29 16025.0 30 20406.0 31 26038.0 32 34384.0 33 46270.0 34 64003.0 35 92246.0 36 157048.0 37 269034.0 38 753489.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69221492188461 18.9967347543777 11.736447944414262 24.57460237932342 2 15.337024827286447 20.099296902022363 40.684732336079 23.878945934612194 3 18.852551867969435 24.706071811489178 29.55134811102804 26.89002820951335 4 12.460969164721758 16.512951502580016 38.10401365558824 32.92206567710999 5 13.426119491153463 37.619346991295785 35.55057398579926 13.40395953175149 6 32.00608237518587 36.72940773330559 18.129761416691654 13.134748474816888 7 28.08537538560301 31.36003008867338 22.36956878618665 18.185025739536957 8 25.513838082373297 36.18665582431471 20.634718468289183 17.66478762502281 9 26.81712865215779 14.872334357791821 19.916415447903777 38.394121542146614 10 15.270598096589952 28.47314209497472 34.34293807951983 21.9133217289155 11 34.77294839708483 22.08314720874306 24.44612381517675 18.697780578995353 12 23.503006561383476 24.366027661466145 31.522053725878212 20.608912051272167 13 29.262624865510617 21.581644081426816 25.72165257613721 23.434078476925354 14 21.583069731122688 21.492377107517267 27.4982849792331 29.426268182126947 15 23.55053933491317 29.86398261976322 23.487084565729894 23.098393479593717 16 22.80849050591021 27.52991876216484 26.963778809524975 22.69781192239997 17 22.34240584498172 27.805526866627645 27.340858175220735 22.5112091131699 18 22.17712440946614 26.879151916687594 28.981027753487286 21.962695920358975 19 23.744711696547643 26.519260364830295 27.485854055659658 22.250173882962404 20 23.940964970273935 26.58808621139908 27.76404091011114 21.706907908215843 21 23.57335805684001 26.256657642015934 27.1396953553525 23.03028894579155 22 23.72771114239622 27.094949356634558 27.109559489219986 22.067780011749235 23 23.11139341358148 26.783140912787506 27.509070292703196 22.596395380927817 24 23.522767505479173 26.302536038800746 27.819062985055243 22.35563347066484 25 23.317823985136517 26.58103741668551 27.744636835529214 22.356501762648758 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 769.0 1 769.0 2 1104.0 3 1439.0 4 1439.0 5 1439.0 6 2291.0 7 3143.0 8 3143.0 9 3143.0 10 3640.5 11 4138.0 12 4138.0 13 4138.0 14 5617.0 15 7096.0 16 7096.0 17 7096.0 18 12507.0 19 17918.0 20 17918.0 21 17918.0 22 27852.0 23 37786.0 24 37786.0 25 37786.0 26 54798.0 27 71810.0 28 71810.0 29 71810.0 30 92035.0 31 112260.0 32 112260.0 33 112260.0 34 128040.5 35 143821.0 36 143821.0 37 143821.0 38 157265.0 39 170709.0 40 170709.0 41 170709.0 42 183227.5 43 195746.0 44 195746.0 45 195746.0 46 204922.5 47 214099.0 48 214099.0 49 214099.0 50 209952.0 51 205805.0 52 205805.0 53 205805.0 54 186877.0 55 167949.0 56 167949.0 57 167949.0 58 145565.5 59 123182.0 60 123182.0 61 123182.0 62 102357.0 63 81532.0 64 81532.0 65 81532.0 66 63153.0 67 44774.0 68 44774.0 69 44774.0 70 32955.5 71 21137.0 72 21137.0 73 21137.0 74 14819.5 75 8502.0 76 8502.0 77 8502.0 78 5827.5 79 3153.0 80 3153.0 81 3153.0 82 2098.0 83 1043.0 84 1043.0 85 1043.0 86 683.0 87 323.0 88 323.0 89 323.0 90 222.5 91 122.0 92 122.0 93 122.0 94 89.5 95 57.0 96 57.0 97 57.0 98 43.5 99 30.0 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3475462708358384 2 0.2965191049737448 3 0.3177600783230374 4 0.30634610701177956 5 0.2909036752377249 6 0.25104633156793177 7 0.35615252727908625 8 0.3198963831139145 9 0.2630096383968436 10 0.23853368922136572 11 0.2522670771627187 12 0.24927625045549068 13 0.267648471657034 14 0.2595305134517009 15 0.34693589803844493 16 0.4022967107620321 17 0.4185936644524376 18 0.11597083150475815 19 0.13336645623047189 20 0.19812701003391842 21 0.19483099692799372 22 0.15210490111045122 23 0.2540371582751597 24 0.3538331106489911 25 0.39210348504556125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1638343.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.88664064010707 #Duplication Level Percentage of deduplicated Percentage of total 1 82.07545677628086 58.18053409872862 2 11.997476297159189 17.00921581729852 3 3.2106399952023414 6.827744506939905 4 1.1429887676964476 3.2409053612550767 5 0.5510746053126393 1.9531913756342956 6 0.29371875226324556 1.2492441384567194 7 0.1765397079550204 0.8760014785561888 8 0.10711800798542197 0.6074588590517382 9 0.07489550798819078 0.47781818642854335 >10 0.32974675418771776 4.07916408440503 >50 0.022557461243563004 1.090939807353521 >100 0.01536991097719017 2.259656836840974 >500 0.0014677409899688677 0.7055966922735002 >1k 8.633770529228633E-4 1.135846041452328 >5k 8.633770529228634E-5 0.30668271532505065 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5011 0.3058578087738648 No Hit GGTATCAACGCAGAGTACTTTTTTT 3188 0.19458684780903632 No Hit TATCAACGCAGAGTACTTTTTTTTT 2917 0.17804574499967346 No Hit GTCCTACAGTGGACATTTCTAAATT 2025 0.12360049147217647 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1937 0.11822921085511398 No Hit CTGTAGGACGTGGAATATGGCAAGA 1867 0.11395660127335973 No Hit CTTTAGGACGTGAAATATGGCGAGG 1731 0.10565553122880862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 6.10372797393464E-5 5 0.0 0.0 0.0 0.0 6.10372797393464E-5 6 0.0 0.0 0.0 0.0 1.220745594786928E-4 7 0.0 6.10372797393464E-5 0.0 0.0 1.220745594786928E-4 8 0.0 6.10372797393464E-5 0.0 6.10372797393464E-5 1.220745594786928E-4 9 0.0 6.10372797393464E-5 0.0 6.10372797393464E-5 1.831118392180392E-4 10 0.0 6.10372797393464E-5 0.0 6.10372797393464E-5 1.831118392180392E-4 11 0.0 6.10372797393464E-5 0.0 1.220745594786928E-4 1.831118392180392E-4 12 0.0 6.10372797393464E-5 0.0 1.220745594786928E-4 2.441491189573856E-4 13 0.0 6.10372797393464E-5 0.0 1.220745594786928E-4 2.441491189573856E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGCC 35 0.0021678514 16.288422 7 GGTATCA 850 0.0 13.300988 1 GTGCGCC 105 2.7412534E-7 11.754845 11 GCGTGCG 90 7.5780263E-6 11.594899 9 GGCGAGG 565 0.0 11.438338 19 ATTAGGC 75 2.0707327E-4 11.401896 3 GACGTGA 705 0.0 10.781935 7 TGCGCCA 115 8.8525485E-7 10.735646 12 GGACGTG 1225 0.0 10.703492 6 GTATCAA 1925 0.0 10.660531 1 TAGGACC 410 0.0 10.660309 4 AGGACGT 1275 0.0 10.433107 5 GTATAAA 265 0.0 10.396999 1 GTCCTAA 570 0.0 10.334102 1 TTAGGAC 755 0.0 10.319597 3 TGGCGAG 1090 0.0 10.284221 18 GTGTAGG 250 0.0 10.260761 1 ACGTGAA 800 0.0 10.195744 8 TAGGACG 1335 0.0 10.106549 4 GCAATAC 85 6.5832364E-4 10.060496 3 >>END_MODULE