FastQCFastQC Report
Thu 26 May 2016
SRR1512299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638343
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53530.3267325584447213No Hit
GGTATCAACGCAGAGTACTTTTTTT34170.20856438486934664No Hit
TATCAACGCAGAGTACTTTTTTTTT33030.20160613497906119No Hit
CTGTAGGACGTGGAATATGGCAAGA22040.13452616454551947No Hit
GTCCTAAAGTGTGTATTTCTCATTT20240.12353945419243711No Hit
GTCCTACAGTGGACATTTCTAAATT19750.12054862748520914No Hit
CTTTAGGACGTGAAATATGGCGAGG17970.10968399169160549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG508.6893306E-515.20513255
GGTATCA10450.013.9239971
ACCGTGC701.0859861E-412.2202778
GCACTAT657.952042E-411.7048381
TAGGACC3700.011.5576034
CCTACAC2050.011.1250283
AAGCGAC1605.493348E-1010.68131814
AGGACCT7400.010.402165
GGTCTAA750.002624275910.1441931
ATTTAGA4700.010.1172141
TAGACAG2450.010.0850385
CGACTAA1701.5752448E-910.05239217
GTATCAA21500.010.0409821
GGCGAGG4350.010.04016719
TAAGCGA1803.947207E-1010.02595713
CTAGCAC1554.0439772E-89.8091643
GCGACTA1752.5993359E-99.76518216
AGGACGT11900.09.5830675
GTACTAG900.00110272259.5101811
ACTATCT800.0044974359.504668
Produced by FastQC (version 0.11.5)