FastQCFastQC Report
Thu 26 May 2016
SRR1512298_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512298_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120509
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62840.2963439438361261No Hit
GTCCTACAGTGGACATTTCTAAATT50800.23956512327936358No Hit
CTGTAGGACGTGGAATATGGCAAGA45390.21405238081988806No Hit
GTCCTAAAGTGTGTATTTCTCATTT44480.20976095833594668No Hit
GGTATCAACGCAGAGTACTTTTTTT38010.17924941606001202No Hit
CTTTAGGACGTGAAATATGGCGAGG36440.1718455333129923No Hit
TATCAACGCAGAGTACTTTTTTTTT36250.1709495220251364No Hit
GTCCTACAGTGTGCATTTCTCATTT34660.16345132230044768No Hit
CTGTAGGACCTGGAATATGGCGAGA23010.10851168280823142No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAG400.005348428614.2223398
GGTATCA11350.012.9769351
TAGGACC9300.012.7721324
TCCAACG2400.012.27198118
TAGGTCG550.003063111612.0940475
GCCGGTT1650.012.08087211
GTTCGCA550.003109094212.0700099
GTTTAGG3000.012.0364571
CGCGCCT1052.739962E-711.75575912
GGCGAGG12100.011.54705519
TCGCGCG1001.9710606E-611.3802949
ATCGCGC1001.9761155E-611.3778718
TATGTCG1451.198714E-911.14848816
TTCGCAG600.005920578711.07229710
AGGACCT18450.010.8673185
GGACGTG26600.010.8597036
AGGACGT27250.010.8101445
TTAGGAC15150.010.7882793
GACGTGA13700.010.7509657
GGACCTG17200.010.7176446
Produced by FastQC (version 0.11.5)