FastQCFastQC Report
Thu 26 May 2016
SRR1512298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120509
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69590.3281759237994274No Hit
GTCCTACAGTGGACATTTCTAAATT52180.2460729947385274No Hit
CTGTAGGACGTGGAATATGGCAAGA50940.2402253421230469No Hit
GTCCTAAAGTGTGTATTTCTCATTT46040.21711768259413186No Hit
TATCAACGCAGAGTACTTTTTTTTT44100.20796893576023492No Hit
GGTATCAACGCAGAGTACTTTTTTT43970.20735587540538616No Hit
CTTTAGGACGTGAAATATGGCGAGG40160.18938849115943387No Hit
GTCCTACAGTGTGCATTTCTCATTT35910.16934613340476273No Hit
CTGTAGGACCTGGAATATGGCGAGA27380.12911994242891683No Hit
CTGAAGGACCTGGAATATGGCGAGA23510.11086960724995744No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21980.10365435845827584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA456.7474134E-414.7824966
TAGGACC10950.013.6227814
GGGCCGT801.9932231E-613.066676
GGTATCA12550.013.0336131
TGGACCG802.8629975E-511.8768295
TAGGCTA1052.7160422E-711.7637165
TAGAAAT7600.011.1264514
AGGACCT19850.011.1049845
CGCCCTA2150.011.04169816
TCCAACG2500.011.01519918
GTCCTAA13500.010.9188791
TAGACCG700.001489875110.8588145
TTCTACG700.001490755210.8580462
GGACCTG19050.010.8249776
GTCGAGG1252.2545282E-710.63536519
ATTTAGA7800.010.6072981
TGTAGGA30450.010.5772342
GGCGAGG10700.010.56081719
CCAACGT1355.7263605E-810.55095719
GAAATGT7950.010.519086