FastQCFastQC Report
Thu 26 May 2016
SRR1512297_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512297_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534295
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49200.19413683095298695No Hit
GGTATCAACGCAGAGTACTTTTTTT29200.11521941999648817No Hit
GTCCTACAGTGGACATTTCTAAATT29170.11510104388005343No Hit
TATCAACGCAGAGTACTTTTTTTTT29150.11502212646909693No Hit
CTGTAGGACGTGGAATATGGCAAGA26160.10322397353110035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.013.7013731
TATACCG604.089741E-412.6680815
ATACCGT701.0906861E-412.2151666
TAGGACC6700.011.4865864
TTTACGC600.0058707611.0850113
GTCCTAC16300.010.8419171
TCCTACA17500.010.6959052
TTAGGAC10050.010.5886673
GTATCAA26350.010.5288991
GGACGTG13800.010.4645216
GTCTAGA3650.010.4123281
GGCGAGG7400.010.40352419
TAGGACG14650.010.3767914
AGGACGT14200.010.3708765
TAAGACT2300.010.3274454
TAACCTA1303.838104E-710.2319125
GTCCTAA8300.010.1880871
TAAGACG856.588006E-410.0601464
CTTATAC2850.010.0015143
TGTAGGA17800.09.9818482
Produced by FastQC (version 0.11.5)