FastQCFastQC Report
Thu 26 May 2016
SRR1512297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534295
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57780.22799240025332487No Hit
GGTATCAACGCAGAGTACTTTTTTT36780.1451291187490012No Hit
TATCAACGCAGAGTACTTTTTTTTT36170.14272213771482797No Hit
GTCCTACAGTGGACATTTCTAAATT29460.11624534633892267No Hit
CTGTAGGACGTGGAATATGGCAAGA28020.11056329275005476No Hit
GTCCTAAAGTGTGTATTTCTCATTT25870.10207967107223113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.014.6368791
AACCGTC604.0782377E-412.6723177
TAGGACC6900.011.8439884
ACCGATC855.301564E-511.1814558
TGGACCG1408.3182385E-910.8604835
ACCGTGC1605.4023985E-1010.6922668
GTATCAA29050.010.6071651
GTATTAC2100.010.4161921
GTAGGAC17700.010.3078493
ACACCGT1201.515953E-610.2962576
CTAGTAC1201.5194146E-610.2944273
GTCCTAC15250.010.289981
AGGACGT15650.010.2619425
GGACGTG15900.010.1617626
CTAATTG750.002642671310.136053
TAGGACG15950.010.1283074
GCGTAGA856.529752E-410.0698741
GACCGCT856.5690157E-410.063317
ACCGACC856.5690157E-410.063318
CTACGAC1054.0948988E-59.955053
Produced by FastQC (version 0.11.5)