FastQCFastQC Report
Thu 26 May 2016
SRR1512296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020836
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80850.4000819462836173No Hit
CTGTAGGACGTGGAATATGGCAAGA72880.3606428230692644No Hit
GTCCTAAAGTGTGTATTTCTCATTT66990.3314964697778543No Hit
GTATCAACGCAGAGTACTTTTTTTT57210.28310065735170986No Hit
CTTTAGGACGTGAAATATGGCGAGG57060.2823583902899592No Hit
GTCCTACAGTGTGCATTTCTCATTT34770.17205750491380795No Hit
GGTATCAACGCAGAGTACTTTTTTT34330.16988018819933928No Hit
TATCAACGCAGAGTACTTTTTTTTT34310.1697812192577725No Hit
CTGAAGGACCTGGAATATGGCGAGA31980.1582513375652453No Hit
GTCCTTCAGTGTGCATTTCTCATTT27880.13796270454405998No Hit
CTGTAGGACCTGGAATATGGCGAGA26210.12969879792323574No Hit
ATTTAGAAATGTCCACTGTAGGACG25130.12435447507863082No Hit
TTTCTAAATTTTCCACCTTTTTCAG22450.11109263690868533No Hit
GAATATGGCAAGAAAACTGAAAATC22250.11010294749301773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGT707.2400817E-613.57595919
TCCAACG2450.013.18217318
CCAACGA1950.012.67089519
GGTATCA11200.012.5555751
TAGGACC13050.012.3780724
ACCGCTA550.003104355712.0724228
AGGACCG1209.988071E-911.8774865
ATGTCGA1950.011.70057817
GACCGCT658.012808E-411.6944667
GGACCGC658.0144795E-411.6941756
TGTCGAG2050.011.58404718
TATGTCG2000.011.40777816
AAATGTC7750.011.2795017
GGCGAGG16700.011.26723419
CTCCTAC2550.011.1782561
TGTAGGA40150.011.1236792
GCCGGTT1803.6379788E-1211.076838511
AGGACGT40150.011.02846055
CGTTTCC1658.0035534E-1110.94658614
GTCGAGG1658.0035534E-1110.94304719
Produced by FastQC (version 0.11.5)