FastQCFastQC Report
Thu 26 May 2016
SRR1512296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020836
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT83580.4135912068074797No Hit
CTGTAGGACGTGGAATATGGCAAGA80130.396519064387214No Hit
GTCCTAAAGTGTGTATTTCTCATTT69290.3428778980580314No Hit
GTATCAACGCAGAGTACTTTTTTTT65970.32644905375794964No Hit
CTTTAGGACGTGAAATATGGCGAGG64560.3194717433774933No Hit
GGTATCAACGCAGAGTACTTTTTTT41640.20605333634198916No Hit
TATCAACGCAGAGTACTTTTTTTTT40250.1991749949030995No Hit
GTCCTACAGTGTGCATTTCTCATTT37300.1845770760220028No Hit
CTGAAGGACCTGGAATATGGCGAGA36180.17903481529426435No Hit
CTGTAGGACCTGGAATATGGCGAGA28300.1400410523169619No Hit
GTCCTTCAGTGTGCATTTCTCATTT28290.1399915678461785No Hit
ATTTAGAAATGTCCACTGTAGGACG26620.13172766122535426No Hit
TTTCTAAATTTTCCACCTTTTTCAG24660.12202870495181203No Hit
GAATATGGCAAGAAAACTGAAAATC23110.11435861198038832No Hit
ATTCCAGGTCCTTCAGTGTGCATTT20760.10272976134629432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1950.014.12054818
TAGGACC9750.013.742244
GGTATCA10850.013.2336841
CCGCCCT1800.013.18664215
CGCCCTA1800.013.18664216
GTCCCGC1950.012.17921612
AGGCCGT802.8549523E-511.8800326
CCACCTT10550.011.70503113
TCCCGCC2050.011.584249513
ACCTTTT11150.011.49544115
CCCGCCC2100.011.30339514
CCTTTTT11350.011.29287816
CACCTTT11000.011.22118914
GGCGAGG16500.011.22118919
ATGTCGA1451.2296368E-911.13134617
AGGACCG600.005864674711.0863825
CGTCGGT600.00590044811.07732819
TTCCACC11550.010.93915211
TGTAGGA38850.010.859862
TATGTCG1502.1536835E-910.760316
Produced by FastQC (version 0.11.5)