FastQCFastQC Report
Thu 26 May 2016
SRR1512295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929670
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT63610.3296418558613649No Hit
GTATCAACGCAGAGTACTTTTTTTT61270.31751543009944705No Hit
CTGTAGGACGTGGAATATGGCAAGA60040.3111412832245928No Hit
GTCCTAAAGTGTGTATTTCTCATTT58460.30295335471868245No Hit
CTTTAGGACGTGAAATATGGCGAGG48520.2514419563966896No Hit
GGTATCAACGCAGAGTACTTTTTTT36460.18894422362372842No Hit
TATCAACGCAGAGTACTTTTTTTTT35190.18236278741961062No Hit
GTCCTACAGTGTGCATTTCTCATTT30690.1590427378774609No Hit
CTGAAGGACCTGGAATATGGCGAGA26920.13950571859437105No Hit
GTCCTTCAGTGTGCATTTCTCATTT22920.11877678566801578No Hit
ATTTAGAAATGTCCACTGTAGGACG21940.11369819710105873No Hit
CTGTAGGACCTGGAATATGGCGAGA20420.10582120258904372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTG400.005278648314.2504295
GTCGAGG1205.0931703E-1113.46715319
TAGGACC7800.012.9106454
GGTATCA11650.012.4777461
GTGTAGG3400.012.2954491
GGCGAGG13600.011.95268219
GTAAGAC1800.011.6123693
TAGACTG1701.0913936E-1111.1768075
GGACCGT855.322508E-511.1768076
TAAGACG600.00587519811.0836674
GTCCTAA15900.011.0546551
CTAGGAC2350.010.9160763
GTCCTAG3100.010.7269681
CCACCTT7000.010.72342713
AGGACCT18200.010.7008725
TGTCGAG1605.4205884E-1010.68949118
TGGCGAG30350.010.519258518
TTAGGAC19100.010.4959013
GGACCTG18300.010.4866556
GTCCACG1002.3963172E-510.4511311
Produced by FastQC (version 0.11.5)