##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512295_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1929670 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.79219762964652 31.0 30.0 34.0 16.0 34.0 2 29.703085501666088 31.0 30.0 34.0 19.0 34.0 3 30.36849824063182 31.0 31.0 34.0 26.0 34.0 4 34.024146097519264 37.0 35.0 37.0 30.0 37.0 5 34.1872071390445 37.0 35.0 37.0 30.0 37.0 6 34.33707421476211 37.0 35.0 37.0 31.0 37.0 7 34.270396492664545 37.0 35.0 37.0 31.0 37.0 8 34.26698399208155 37.0 35.0 37.0 31.0 37.0 9 35.81224043489301 39.0 37.0 39.0 31.0 39.0 10 35.73355444195121 39.0 37.0 39.0 30.0 39.0 11 35.7594106764369 39.0 37.0 39.0 30.0 39.0 12 35.69347349546814 39.0 37.0 39.0 30.0 39.0 13 35.66984873061197 39.0 37.0 39.0 30.0 39.0 14 36.86024346131722 40.0 38.0 41.0 31.0 41.0 15 36.79616929319521 40.0 38.0 41.0 30.0 41.0 16 36.71890478682884 40.0 38.0 41.0 30.0 41.0 17 36.66576668549545 40.0 37.0 41.0 30.0 41.0 18 36.45273803292791 40.0 37.0 41.0 29.0 41.0 19 36.54458793472459 40.0 37.0 41.0 30.0 41.0 20 36.483409080309066 40.0 37.0 41.0 30.0 41.0 21 36.41940849989895 40.0 37.0 41.0 29.0 41.0 22 36.341762581166726 40.0 37.0 41.0 29.0 41.0 23 36.21210569682899 40.0 37.0 41.0 29.0 41.0 24 36.08305306088606 40.0 37.0 41.0 28.0 41.0 25 35.95958428125016 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1192622179687142 1101 2 0.2316034319509761 1101 3 -0.1924213195379103 1101 4 -0.3358377188698043 1101 5 -0.3974290509038312 1101 6 -0.6285266778574865 1101 7 -0.6266056215377063 1101 8 -0.7027822711018104 1101 9 -0.8117863318597571 1101 10 -0.8207807971015413 1101 11 -0.8574372120260065 1101 12 -0.8299082248169256 1101 13 -1.05605501243911 1101 14 -1.3034657019124793 1101 15 -1.3304096821045093 1101 16 -1.2869279482521137 1101 17 -1.2479126333249937 1101 18 -1.2716278249015005 1101 19 -1.4485280379695311 1101 20 -1.6287586483734628 1101 21 -1.6596291439227997 1101 22 -1.4954756955488406 1101 23 -1.8339334547862407 1101 24 -2.005471816913584 1101 25 -1.8954634055122241 1102 1 1.1749365703463646 1102 2 0.3866651804193175 1102 3 -0.13257027067587046 1102 4 -0.15038432462520035 1102 5 -0.3399186966901837 1102 6 -0.46457094247318054 1102 7 -0.49915006551071883 1102 8 -0.5562160366234892 1102 9 -0.6687170459333274 1102 10 -0.6711288452517934 1102 11 -0.7220498788364793 1102 12 -0.927794300724976 1102 13 -0.8448391418825807 1102 14 -1.3458607882636073 1102 15 -1.2321097046836513 1102 16 -1.34240350922218 1102 17 -1.5092764551283722 1102 18 -1.3165707050092124 1102 19 -1.2852214900475971 1102 20 -1.5028685005052935 1102 21 -1.6512392564120333 1102 22 -1.6280401907507027 1102 23 -1.640028458683446 1102 24 -1.702195942209137 1102 25 -1.8964941537957216 1103 1 1.2829144347613912 1103 2 0.47423722577649485 1103 3 0.02544295671341601 1103 4 -0.061047799498858524 1103 5 -0.18286870817679812 1103 6 -0.21205221251479145 1103 7 -0.4051150344347647 1103 8 -0.42980680939902527 1103 9 -0.5059920479512527 1103 10 -0.555612712341194 1103 11 -0.4810547370825944 1103 12 -0.6740301054400746 1103 13 -0.6590994235332914 1103 14 -0.8167254228622056 1103 15 -0.8871208893178917 1103 16 -0.814096196226842 1103 17 -1.1121674699977575 1103 18 -0.8463351531690151 1103 19 -1.0450814944390387 1103 20 -0.8731953243796156 1103 21 -1.0613411006489955 1103 22 -1.1066084785449135 1103 23 -1.1070452178545338 1103 24 -1.1684416587171853 1103 25 -1.2931572211343862 1104 1 1.2856323313410485 1104 2 0.42902847674833566 1104 3 -0.045994391393733736 1104 4 -0.07917327182785527 1104 5 -0.18912003341642958 1104 6 -0.2917856966309458 1104 7 -0.3815733417163756 1104 8 -0.33548563111827434 1104 9 -0.47461581038131584 1104 10 -0.4604096720375992 1104 11 -0.3352908825517744 1104 12 -0.3137617410168474 1104 13 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0.16288489077279422 1107 11 9.644193453937078E-4 1107 12 0.0014137398778899524 1107 13 0.1345852001335217 1107 14 -0.10627528599059133 1107 15 -0.16684684752804202 1107 16 -0.30109945738509225 1107 17 -0.1747664072445616 1107 18 -0.04156738085172407 1107 19 -0.1854671110081867 1107 20 -0.20348291960039688 1107 21 -0.1107669613913842 1107 22 -0.31587836598114905 1107 23 -0.20991787344997448 1107 24 -0.47421645281886526 1107 25 -0.2359000399618978 1108 1 -0.06580612194069246 1108 2 -0.3706773393469689 1108 3 -0.6084408883525079 1108 4 -0.4104110969872039 1108 5 -0.41418132172990596 1108 6 -0.318944701706279 1108 7 -0.2680660600021625 1108 8 -0.41591180023426233 1108 9 -0.5751896015107434 1108 10 -0.5831201394189662 1108 11 -0.6246419552629021 1108 12 -0.6389793973517399 1108 13 -0.685140489167992 1108 14 -0.8993888655375102 1108 15 -0.8040999807856792 1108 16 -0.7572529525193943 1108 17 -0.7413664190696068 1108 18 -0.76821329810371 1108 19 -0.6127520354416092 1108 20 -0.853686598401886 1108 21 -0.7868086643796346 1108 22 -0.7374651352730268 1108 23 -0.9191257925909326 1108 24 -0.8921374584933659 1108 25 -1.060392737997276 1109 1 0.9546273282091775 1109 2 0.2317200516210285 1109 3 -0.19819259823353264 1109 4 -0.11208753717468056 1109 5 -0.2587964416447335 1109 6 -0.30231690920246024 1109 7 -0.34024574272172003 1109 8 -0.3994402805714401 1109 9 -0.5641296757920884 1109 10 -0.6383206744723324 1109 11 -0.563333842917217 1109 12 -0.7099099322628106 1109 13 -0.7031636118564037 1109 14 -0.9667372854095149 1109 15 -0.9149437376986143 1109 16 -0.8893687030882731 1109 17 -0.9667395398822265 1109 18 -1.0168501151053633 1109 19 -1.1164895177671283 1109 20 -1.079827629466287 1109 21 -1.365992811483089 1109 22 -1.2460390550434823 1109 23 -1.388473935621974 1109 24 -1.3801992742337177 1109 25 -1.5986616023154525 1110 1 0.6317977669784263 1110 2 0.07368850443118546 1110 3 -0.1577265292200174 1110 4 -0.1762028777759923 1110 5 -0.059456514755005685 1110 6 -0.15982793134719486 1110 7 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1.1179264663870327 1201 17 1.0396967002726 1201 18 1.3400498083025525 1201 19 1.0955215814225525 1201 20 0.939603147972683 1201 21 0.996951330661588 1201 22 1.1060707983655576 1201 23 1.3132569282162763 1201 24 1.166278049552524 1201 25 1.164269108232034 1202 1 2.4695098573144847 1202 2 1.6993251102067113 1202 3 1.2613795567287625 1202 4 0.9721791611641422 1202 5 0.7516845428176779 1202 6 0.7929279952523274 1202 7 0.7880637505393508 1202 8 0.8132723930328893 1202 9 0.893510633699087 1202 10 0.7927975871845021 1202 11 0.8984439029105715 1202 12 0.8810558446141172 1202 13 1.0666433390366379 1202 14 1.1519387662181941 1202 15 1.200155131011428 1202 16 1.2518638648518916 1202 17 1.2330581333600605 1202 18 1.3504135821921395 1202 19 1.2927707862335183 1202 20 1.3082610338279181 1202 21 1.161431476166804 1202 22 1.2409745780501709 1202 23 1.2771975063520742 1202 24 1.368457914747495 1202 25 1.3943410903390614 1203 1 2.5544802973033818 1203 2 1.7826130031836485 1203 3 1.1402613348431991 1203 4 0.949414191139212 1203 5 0.7930519559859519 1203 6 0.7234229276365554 1203 7 0.6798850052120073 1203 8 0.6219385737341838 1203 9 0.8204129676299345 1203 10 0.8204075973794005 1203 11 0.834029538742648 1203 12 0.884746500848955 1203 13 0.812982904313813 1203 14 0.940124878849744 1203 15 0.9929198675654476 1203 16 0.9457059399413268 1203 17 1.0260052366346173 1203 18 1.220734500926099 1203 19 1.1503652057599822 1203 20 1.1181378908944382 1203 21 1.1109836011411858 1203 22 1.194993779735313 1203 23 1.1190917855365896 1203 24 1.191284884269166 1203 25 1.1208592349198128 1204 1 2.5729923493364524 1204 2 1.6984273433446226 1204 3 1.230738880421221 1204 4 0.9873233246379911 1204 5 0.8859959525860077 1204 6 0.7274486807255229 1204 7 0.8826191273092903 1204 8 0.8258835532860473 1204 9 0.9007110592619156 1204 10 0.8743076544179473 1204 11 0.9487724490007636 1204 12 0.8950266110489125 1204 13 0.9096596324636366 1204 14 1.0814909954693377 1204 15 1.0488859874159786 1204 16 1.1771802806739302 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-0.9457875675636629 2314 15 -0.8986211995449409 2314 16 -1.161983785420027 2314 17 -1.3520151673951588 2314 18 -2.1210317669626733 2314 19 -1.9632456495329862 2314 20 -2.1097328455372875 2314 21 -1.549538954185472 2314 22 -1.257181408006602 2314 23 -1.0949421674672308 2314 24 -1.1217297624537537 2314 25 -1.243475675844735 2315 1 -2.7707360646512917 2315 2 -1.2288969351577137 2315 3 -0.6887753624493094 2315 4 -0.6211646851362218 2315 5 -0.4961326321864874 2315 6 -0.5625760780897977 2315 7 -0.5422794835916704 2315 8 -0.5700931727006449 2315 9 -0.636593682673869 2315 10 -0.6147396830672989 2315 11 -0.7812807127265415 2315 12 -0.8365773481425549 2315 13 -0.7441871467574757 2315 14 -0.8736255936255759 2315 15 -1.2754627187766658 2315 16 -1.1755675116825728 2315 17 -1.1659337239489744 2315 18 -0.8736507830425566 2315 19 -0.9846850890548495 2315 20 -0.9832370000821911 2315 21 -0.9477830502565112 2315 22 -0.9543074451355906 2315 23 -0.9658387178608407 2315 24 -0.830740914104247 2315 25 -0.5751149334992078 2316 1 1.0106901491610039 2316 2 0.2690172396151489 2316 3 -0.34559911336371485 2316 4 -0.2692106036418025 2316 5 -0.6274465710539019 2316 6 -0.7604079805782646 2316 7 -0.6939102499616254 2316 8 -0.7758926210899233 2316 9 -0.868558441651011 2316 10 -0.9335885343569998 2316 11 -0.9792381629874853 2316 12 -1.0053319127590186 2316 13 -1.0036773665415808 2316 14 -1.0691649168016824 2316 15 -1.048671179452903 2316 16 -0.9832942072104203 2316 17 -0.9525088769492172 2316 18 -0.8662818098353071 2316 19 -1.1781707882308368 2316 20 -1.0252590831655013 2316 21 -1.0374117234726867 2316 22 -1.1843259175360288 2316 23 -1.0148328477622215 2316 24 -1.2331838546403517 2316 25 -1.1637737945884439 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59771.0 3 2.0 4 493.0 5 2481.0 6 2853.0 7 2730.0 8 2480.0 9 2447.0 10 2337.0 11 2411.0 12 2295.0 13 2493.0 14 2366.0 15 2539.0 16 2461.0 17 2822.0 18 2949.0 19 3584.0 20 4400.0 21 5317.0 22 6748.0 23 7926.0 24 9268.0 25 10789.0 26 12099.0 27 13500.0 28 15475.0 29 18910.0 30 23907.0 31 30615.0 32 40425.0 33 54464.0 34 75407.0 35 108374.0 36 186084.0 37 319438.0 38 887010.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9605558455929 19.35013030881004 12.0233536683022 24.66596017729486 2 14.530982420123955 19.586814443303926 42.42771201746294 23.454491119109182 3 19.011393798754263 24.35428382504764 29.295235481197995 27.339086895000097 4 12.288356281869357 16.291693305680738 38.11307416821303 33.306876244236875 5 13.076314229007648 37.977506367617735 35.638546941074914 13.3076324622997 6 32.175494099892724 36.734538030561595 17.941209320688948 13.148758548856732 7 28.454894284047068 31.140673415872943 22.015202876647212 18.389229423432777 8 25.049891120654117 37.67220169164558 20.16105513937024 17.11685204833006 9 26.93271458341668 14.719590843651172 20.13652746005826 38.21116711287389 10 15.589558619271074 28.222831456285263 34.43978919439472 21.747820730048943 11 34.5719148193402 21.48597569591635 25.384218969486906 18.55789051525655 12 24.512983977042975 23.729862212128076 31.83947120709324 19.917682603735713 13 29.707789508687338 21.83327791171336 25.587954110898664 22.87097846870064 14 20.71971816424383 22.467579900135544 27.294136716941786 29.518565218678837 15 22.624724270727924 32.360866567312264 22.79710381564593 22.217305346313886 16 21.802186436021877 27.53143548078284 28.815233045027345 21.85114503816794 17 21.58226023498018 28.795219218044082 28.1427219942405 21.479798552735243 18 21.51192804107847 26.486783198170833 31.132184620684612 20.86910414006609 19 23.958510095624185 26.182406748648045 27.52634504102961 22.332738114698163 20 24.155948010406227 26.807770398334174 28.413673491642307 20.622608099617295 21 22.74333030118005 26.300606846020607 27.13071072842291 23.825352124376437 22 23.597501733465645 28.371071676209375 27.156452021407784 20.874974568917196 23 21.853757930545772 28.053454943693907 28.427356928070758 21.66543019768957 24 23.68135179673959 26.052009062870674 29.031971215973286 21.23466792441645 25 22.678797539144067 27.230839980897464 28.626074127406415 21.46428835255205 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 793.0 1 793.0 2 1248.5 3 1704.0 4 1704.0 5 1704.0 6 2694.5 7 3685.0 8 3685.0 9 3685.0 10 4184.0 11 4683.0 12 4683.0 13 4683.0 14 6329.5 15 7976.0 16 7976.0 17 7976.0 18 13949.0 19 19922.0 20 19922.0 21 19922.0 22 32866.5 23 45811.0 24 45811.0 25 45811.0 26 68379.5 27 90948.0 28 90948.0 29 90948.0 30 120080.5 31 149213.0 32 149213.0 33 149213.0 34 168015.5 35 186818.0 36 186818.0 37 186818.0 38 199059.5 39 211301.0 40 211301.0 41 211301.0 42 222734.0 43 234167.0 44 234167.0 45 234167.0 46 243082.0 47 251997.0 48 251997.0 49 251997.0 50 242389.0 51 232781.0 52 232781.0 53 232781.0 54 206440.0 55 180099.0 56 180099.0 57 180099.0 58 156088.0 59 132077.0 60 132077.0 61 132077.0 62 109935.0 63 87793.0 64 87793.0 65 87793.0 66 68325.5 67 48858.0 68 48858.0 69 48858.0 70 36183.5 71 23509.0 72 23509.0 73 23509.0 74 16677.5 75 9846.0 76 9846.0 77 9846.0 78 6796.5 79 3747.0 80 3747.0 81 3747.0 82 2512.0 83 1277.0 84 1277.0 85 1277.0 86 840.5 87 404.0 88 404.0 89 404.0 90 279.5 91 155.0 92 155.0 93 155.0 94 110.0 95 65.0 96 65.0 97 65.0 98 53.0 99 41.0 100 41.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3392289873398042 2 0.28994594930739453 3 0.31051941523680215 4 0.3005695274321516 5 0.28403820342338326 6 0.24382407354625402 7 0.3563303570040473 8 0.3099493695813274 9 0.255432275985013 10 0.23237133810444272 11 0.24491234252488767 12 0.2426321599029886 13 0.26066633154891766 14 0.2519083573875326 15 0.3414055252970715 16 0.3958189742287541 17 0.4133349225515243 18 0.11592655739064192 19 0.1371737136401561 20 0.202107096031964 21 0.18894422362372842 22 0.14997382972218046 23 0.2505091544150036 24 0.34508491089149956 25 0.3841071271253634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1929670.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.64434274210873 #Duplication Level Percentage of deduplicated Percentage of total 1 83.07823615418921 56.197726808238244 2 11.081816962574983 14.992444496434732 3 2.957962453046429 6.002682779764841 4 1.1438291838643981 3.0949429340699943 5 0.5497399904353116 1.8593400166024898 6 0.30957969596161417 1.2564789035775148 7 0.19304507936145815 0.914088525910284 8 0.1300356178421462 0.7036939121596806 9 0.09010800738013819 0.5485767241527477 >10 0.4110049806309052 4.852952903093656 >50 0.02649720282089075 1.2411579752515418 >100 0.02322455518017055 3.2394814498565654 >500 0.0026906888270500605 1.305218831022606 >1k 0.0019219205907500378 2.5255690564342705 >5k 3.0750729452000603E-4 1.2656446834307393 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6361 0.3296418558613649 No Hit GTATCAACGCAGAGTACTTTTTTTT 6127 0.31751543009944705 No Hit CTGTAGGACGTGGAATATGGCAAGA 6004 0.3111412832245928 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5846 0.30295335471868245 No Hit CTTTAGGACGTGAAATATGGCGAGG 4852 0.2514419563966896 No Hit GGTATCAACGCAGAGTACTTTTTTT 3646 0.18894422362372842 No Hit TATCAACGCAGAGTACTTTTTTTTT 3519 0.18236278741961062 No Hit GTCCTACAGTGTGCATTTCTCATTT 3069 0.1590427378774609 No Hit CTGAAGGACCTGGAATATGGCGAGA 2692 0.13950571859437105 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2292 0.11877678566801578 No Hit ATTTAGAAATGTCCACTGTAGGACG 2194 0.11369819710105873 No Hit CTGTAGGACCTGGAATATGGCGAGA 2042 0.10582120258904372 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCTG 40 0.0052786483 14.250429 5 GTCGAGG 120 5.0931703E-11 13.467153 19 TAGGACC 780 0.0 12.910645 4 GGTATCA 1165 0.0 12.477746 1 GTGTAGG 340 0.0 12.295449 1 GGCGAGG 1360 0.0 11.952682 19 GTAAGAC 180 0.0 11.612369 3 TAGACTG 170 1.0913936E-11 11.176807 5 GGACCGT 85 5.322508E-5 11.176807 6 TAAGACG 60 0.005875198 11.083667 4 GTCCTAA 1590 0.0 11.054655 1 CTAGGAC 235 0.0 10.916076 3 GTCCTAG 310 0.0 10.726968 1 CCACCTT 700 0.0 10.723427 13 AGGACCT 1820 0.0 10.700872 5 TGTCGAG 160 5.4205884E-10 10.689491 18 TGGCGAG 3035 0.0 10.5192585 18 TTAGGAC 1910 0.0 10.495901 3 GGACCTG 1830 0.0 10.486655 6 GTCCACG 100 2.3963172E-5 10.451131 1 >>END_MODULE