FastQCFastQC Report
Thu 26 May 2016
SRR1512295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1929670
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66460.344411220571393No Hit
GTATCAACGCAGAGTACTTTTTTTT65450.33917716500748835No Hit
CTGTAGGACGTGGAATATGGCAAGA63990.33161110448936865No Hit
GTCCTAAAGTGTGTATTTCTCATTT60850.31533889214217975No Hit
CTTTAGGACGTGAAATATGGCGAGG52530.27222271165536077No Hit
GGTATCAACGCAGAGTACTTTTTTT43300.22439069892779595No Hit
TATCAACGCAGAGTACTTTTTTTTT42130.21832748604683705No Hit
GTCCTACAGTGTGCATTTCTCATTT31250.16194478848715066No Hit
CTGAAGGACCTGGAATATGGCGAGA31180.16158203216093944No Hit
CTGTAGGACCTGGAATATGGCGAGA23630.12245617126244383No Hit
ATTTAGAAATGTCCACTGTAGGACG23480.12167883627770551No Hit
GTCCTTCAGTGTGCATTTCTCATTT23230.12038327796980831No Hit
TTTCTAAATTTTCCACCTTTTTCAG21150.10960423284810356No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19880.10302279664398575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCA400.00526524714.2558956
GGTATCA12350.014.094081
TAGGACC7950.013.1483864
GTATTAT2100.012.2291731
ATTTAGA7600.011.764321
TAGAAAT7800.011.5737984
AAATGTC7450.011.4815557
CGCCCTA1354.771209E-911.25311816
AGGACGT32050.011.1191775
GATCGTG600.005857336311.0882057
CGTATTC600.00586525811.0861923
GGACGTG31450.011.0299996
GACGTGG16450.010.9774687
TGTAGGA31650.010.9285692
TTAGGAC17600.010.9062213
AATGTCC7950.010.8789948
TAGGACT2650.010.75777154
GTCTTAA2300.010.7522181
TTTAGAA8950.010.7234372
GTCTATA1605.329639E-1010.7005251
Produced by FastQC (version 0.11.5)