FastQCFastQC Report
Thu 26 May 2016
SRR1512294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701267
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT57160.335984886558077No Hit
GTATCAACGCAGAGTACTTTTTTTT56300.3309298305321857No Hit
CTGTAGGACGTGGAATATGGCAAGA52330.30759428120336196No Hit
GTCCTAAAGTGTGTATTTCTCATTT50540.29707271110296035No Hit
CTTTAGGACGTGAAATATGGCGAGG42970.2525764621308707No Hit
GGTATCAACGCAGAGTACTTTTTTT33030.19414941922696438No Hit
GTCCTACAGTGTGCATTTCTCATTT32950.19367918145711402No Hit
TATCAACGCAGAGTACTTTTTTTTT32640.1918570100989439No Hit
CTGTAGGACCTGGAATATGGCGAGA23720.13942549876062957No Hit
CTGAAGGACCTGGAATATGGCGAGA23000.13519335883197642No Hit
GTCCTTCAGTGTGCATTTCTCATTT21010.12349619430694887No Hit
ATTTAGAAATGTCCACTGTAGGACG20200.11873503688721405No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17210.10115990023905712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGG801.2875171E-714.2510195
GTGCGTT400.00530172214.24092511
TAGGACC9550.014.2261484
ATACCGA500.0014991713.3005586
GGTATCA10000.013.0163151
AATGTCC7750.011.8687388
GTCCTAG2500.011.4011521
GTCTTGT1751.8189894E-1211.4011521
TAATACT2350.011.3199594
AGGACCT17750.011.240245
CCACCTT6650.011.14464113
AAATGTC8100.011.1431017
GTATTAG1803.6379788E-1211.0844531
TGTAGGA31050.011.0764762
GGACCTG17750.010.8652456
GTCCTAA13250.010.8275091
GTCCTAC27050.010.8180981
CACCTTT6950.010.80795214
TGTCCAC8600.010.70734510
ACCTTTT7200.010.69679415